Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	715,111	A→G	I269T (ATC→ACC)	speF ←	ornithine decarboxylase SpeF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	715,111	0	A	G	100.0%	64.3 / NA	18	I269T (ATC→ACC)	speF	ornithine decarboxylase SpeF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/9);  total (9/9)

ATAAATCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGATCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGT  >  NZ_CP009273/715023‑715182                                                                                         |                                                                        ataAATCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTc                                                                       <  1:870116/91‑1 (MQ=255)        gTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCAc                                                                >  1:395030/1‑91 (MQ=255)         tACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACg                                                               <  1:124469/91‑1 (MQ=255)                   ggTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACtt                                                     <  1:846510/91‑1 (MQ=255)                   ggTGCCTAACTGAACCACAGGGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACtt                                                     <  2:165806/91‑1 (MQ=255)                     tGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTttc                                                   <  1:170666/91‑1 (MQ=255)                     tGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTttc                                                   >  2:222671/1‑91 (MQ=255)                             tGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAAc                                           <  1:830440/91‑1 (MQ=255)                                  cacaGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTgc                                      <  1:701886/91‑1 (MQ=255)                                        gAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCa                                >  1:207251/1‑91 (MQ=255)                                        gAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCa                                >  1:211659/1‑91 (MQ=255)                                        gAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCa                                >  1:61763/1‑91 (MQ=255)                                              gAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCa                          <  1:766457/91‑1 (MQ=255)                                                     cGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATaa                   >  2:542029/1‑91 (MQ=255)                                                       aGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAg                 >  1:132474/1‑91 (MQ=255)                                                        gCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGc                >  1:51502/1‑91 (MQ=255)                                                           tctTTTGCCCGCTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCAt             <  2:567797/91‑1 (MQ=255)                                                                      cTGCGGTGCGACTTCCGCGGTCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGt  >  2:730464/1‑91 (MQ=255)                                                                                         |                                                                        ATAAATCGTACCGTCGTAGGTGCCTAACTGAATCACAGCGAGGCGGAAAGGACGAGCCTCTTTTGCCCGCTGCGGTGCGACTTCCGCGATCAGCTCACGCAGGTAACTTTCTTCAAAACAGTGCGCATCAATGCCACCGATAAAGCCATACGGGTTACGT  >  NZ_CP009273/715023‑715182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑