Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	736,034	A→T	I358F (ATC→TTC)	phrB →	deoxyribodipyrimidine photo‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	736,034	0	A	T	100.0%	116.2 / NA	30	I358F (ATC→TTC)	phrB	deoxyribodipyrimidine photo‑lyase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (18/12);  total (18/12)

CCGCTATGCGTCAGCTTAACAGCACTGGCTGGATGCATAACAGGCTACGGATGATTACAGCCAGTTTTCTGGTGAAAGATTTATTGATCGACTGGCGCGAAGGCGAGCGATATTTCATGTCGCAGCTGATTGATGGTGATTTGGCAGCCAATAACGGTGGCTGGCAGTGGG  >  NZ_CP009273/735948‑736118                                                                                       |                                                                                     ccGCTATGCGTCAGCTTAACAGCACTGGCTGGATGCATAACAGGCTACGGATGATTACAGCCAGTTTTCTGGTGAAAGATTTATTGTTCGa                                                                                  >  1:695976/1‑91 (MQ=255)    cTATGCGTCAGCTTAACAGCACTGGCTGGATGCATAACAGGCTACGGATGATTACAGCCAGTTTTCTGGTGAAAGATTTATTGTTCGACTg                                                                               >  2:61945/1‑91 (MQ=255)    cTATGCGGCAGCTTAACAGCACTGGCTGGATGCATAACAGGCTACGGATGATTACAGCCAGTTTTCTGGTGAAAGATTTATTGTTCGACTg                                                                               >  1:809304/1‑91 (MQ=255)               cTTAACAGCACTGGCTGGATGCATAACAGGCTACGGATGATTACAGCCAGTTTTCTGGTGAAAGATTTATTGTTCGACTGGCGCGAAGgcg                                                                    <  1:369128/91‑1 (MQ=255)                ttAACAGCACTGGCTGGATGCATAACAGGCTACGGATGATTACAGCCAGTTTTCTGGTGAAAGATTTATTGTTCGACTGGCGCGAAGgcga                                                                   <  2:10361/91‑1 (MQ=255)                  aaCAGCACTGGCTGGATGCATAACAGGCTACGGATGATTACAGCCAGTTTTCTGGTGAAAGATTTATTGTTCGACTGGCGCGAAGgcgagc                                                                 <  2:567256/91‑1 (MQ=255)                  aaCAGCACTGGCTGGATGCATAACAGGCTACGGATGATTACAGCCAGTTTTCTGGTGAAAGATTTATTGTTCGACTGGCGCGAAGgcgagc                                                                 <  2:17548/91‑1 (MQ=255)                      gCACTGGCTGGATGCATAACAGGCTACGGATGATTACAGCCAGTTTTCTGGTGAAAGATTTATTGTTCGACTGGCGCGAAGGCGAGCGata                                                             >  1:487971/1‑91 (MQ=255)                      gCACTGGCTGGATGCATAACAGGCTACGGATGATTACAGCCAGTTTTCTGGTGAAAGATTTATTGTTCGACTGGCGCGAAGGCGAGCGata                                                             >  2:210551/1‑91 (MQ=255)                                gATGCATAACAGGCTACGGATGATTACAGCCAGTTTTCTGGTGAAAGATTTATTGTTCGACTGGCGCGAAGgcg                                                                    >  2:825821/1‑74 (MQ=255)                                gATGCATAACAGGCTACGGATGATTACAGCCAGTTTTCTGGTGAAAGATTTATTGTTCGACTGGCGCGAAGgcg                                                                    <  1:825821/74‑1 (MQ=255)                                 aTGCATAACAGGCTACGGATGATTACAGCCAGTTTTCTGGTGAAAGATTTATTGTTCGACTGGCGCGAAGGCGAGCGATATTTCATGTCGc                                                  <  2:400377/91‑1 (MQ=255)                                  tGCATAACAGGCTACGGATGATTACAGCCAGTTTTCTGGTGAAAGATTTATTGTTCGACTGGCGCGAAGGCGAGCGATATTTCATGTCGCa                                                 <  2:442868/91‑1 (MQ=255)                                    cATAACAGGCTACGGATGATTACAGCCAGTTTTCTGGTGAAAGATTTATTGTTCGACTGGCGCGAAGGCGAGCGATATTTCATGTCGCAGc                                               <  1:726441/91‑1 (MQ=255)                                    cATAACAGGCTACGGATGATTACAGCCAGTTTTCTGGTGAAAGATTTATTGTTCGACTGGCGCGAAGGCGAGCGATATTTCATGTCGCAGc                                               <  2:176061/91‑1 (MQ=255)                                     aTAACAGGCTACGGATGATTACAGCCAGTTTTCTGGTGAAAGATTTATTGTTCGACTGGCGCGAAGGCGAGCGATATTTCATGTCGCAGCt                                              >  2:319148/1‑91 (MQ=255)                                     aTAACAGGCTACGGATGATTACAGCCAGTTTTCTGGTGAAAGATTTATTGTTCGACTGGCGCGAAGGCGAGCGATATTTCATGTCGCAGCt                                              >  2:864845/1‑91 (MQ=255)                                           ggCTACGGATGATTACAGCCAGTTTTCTGGTGAAAGATTTATTGTTCGACTGGCGCGAAGGCGAGCGATATTTCATGTCGCAGCtgattga                                        >  2:118170/1‑91 (MQ=255)                                             cTACGGATGATTACAGCCAGTTTTCTGGTGAAAGATTTATTGTTCGACTGGCGCGAAGGCGAGCGATATTTCATGTCGCAGCTGATTGATg                                      >  2:309735/1‑91 (MQ=255)                                             cTACGGATGATTACAGCCAGTTTTCTGGTGAAAGATTTATTGTTCGACTGGCGCGAAGGCGAGCGATATTTCATGTCGCAGCTGATTGATg                                      >  1:535671/1‑91 (MQ=255)                                              tACGGATGATTACAGCCAGTTTTCTGGTGAAAGATTTACTGTTCGACTGGCGCGAAGGCGAGCGATATTTCATGTCGCAGCTGATTGATgg                                     >  1:329897/1‑91 (MQ=255)                                               aCGGATGATTACAGCCAGTTTTCTGGTGAAAGATTTATTGTTCGACTGGCGCGAAGGCGAGCGATATTTCATGTCGCAGCTGATTGATGGt                                    >  1:447554/1‑91 (MQ=255)                                               aCGGATGATTACAGCCAGTTTTCTGGTGAAAGATTTATTGTTCGACTGGCGCGAAGGCGAGCGATATTTCATGTCGCAGCTGATTGATGGt                                    >  1:349493/1‑91 (MQ=255)                                                cGGATGATTACAGCCAGTTTTCTGGTGAAAGATTTATTGTTCGACTGGCGCGAAGGCGAGCGATATTTCATGTCGCAGCTGATTGATGGTg                                   >  1:482756/1‑91 (MQ=255)                                                       ttACAGTCAGTTTTCTGGTGAAAGATTTATTGTTCGACTGGCGCGAAGGCGAGCGATATTTCATGTCGCAGCTGATTGATGGTGATTTGGc                            <  1:748413/91‑1 (MQ=255)                                                         aCAGCCAGTTTTCTGGTGAAAGATTTATTGTTCGACTGGCGCGAAGGCGAGCGATATTTCATGTCGCAGCTGATTGATGGTGATTTGGCAg                          <  1:745229/91‑1 (MQ=255)                                                                          gAAAGATTTATTGTTCGACTGGCGCGAAGGCGAGCGATATTTCATGTCGCAGCTGATTGATGGTGATTTGGCAGCCAATAACGGtggctgg         >  1:823999/1‑91 (MQ=255)                                                                                 ttATTGTTCGACTGGCGCGAAGGCGAGCGATATTTCATGTCGCAGCTGATTGATGGt                                    <  1:599640/57‑1 (MQ=255)                                                                                 ttATTGTTCGACTGGCGCGAAGGCGAGCGATATTTCATGTCGCAGCTGATTGATGGt                                    >  2:599640/1‑57 (MQ=255)                                                                                 ttATTGTTCGACTGGCGCGAAGGCGAGCGATATTTCATGTCGCAGCTGATTGATGGTGATTTGGCAGCCAATAACGGTGGCTGGCAGTggg  >  1:501415/1‑91 (MQ=255)                                                                                       |                                                                                     CCGCTATGCGTCAGCTTAACAGCACTGGCTGGATGCATAACAGGCTACGGATGATTACAGCCAGTTTTCTGGTGAAAGATTTATTGATCGACTGGCGCGAAGGCGAGCGATATTTCATGTCGCAGCTGATTGATGGTGATTTGGCAGCCAATAACGGTGGCTGGCAGTGGG  >  NZ_CP009273/735948‑736118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑