Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	737,899	A→G	N38N (AAT→AAC)	dtpD ←	dipeptide permease DtpD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	737,899	0	A	G	100.0%	110.1 / NA	30	N38N (AAT→AAC)	dtpD	dipeptide permease DtpD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/20);  total (10/20)

GCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACA  >  NZ_CP009273/737815‑737974                                                                                     |                                                                            gCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGGTTGGTg                                                                       <  1:447961/91‑1 (MQ=255) gCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGGTTGGTg                                                                       <  2:145461/91‑1 (MQ=255) gCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGGTTGGTg                                                                       <  1:740438/91‑1 (MQ=255)       gAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGGTTGGTGAGATag                                                                 <  2:160243/91‑1 (MQ=255)        aGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGGTTGGTGAGATaga                                                                <  1:17688/91‑1 (MQ=255)                   gACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAgg                                                     >  1:81751/1‑91 (MQ=255)                              gCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCATGcc                                          >  2:458740/1‑91 (MQ=255)                              gCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCATGcc                                          >  2:492906/1‑91 (MQ=255)                                  aCAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTaa                                      <  2:642314/91‑1 (MQ=255)                                   cAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTaaa                                     <  1:360631/91‑1 (MQ=255)                                                  aaCTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTccc                      <  1:148187/91‑1 (MQ=255)                                                   aCTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCa                     >  2:703749/1‑91 (MQ=255)                                                      cGTAGGCGTGAGTATCGTTGTATTTTAGTTGGTTGGTGAGATaga                                                                <  2:133463/45‑1 (MQ=255)                                                      cGTAGGCGTGAGTATCGTTGTATTTTAGTTGGTTGGTGAGATaga                                                                >  1:133463/1‑45 (MQ=255)                                                        tAGGCGTGAGTATCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGAttt                >  1:362539/1‑91 (MQ=255)                                                          ggCGTGAGTATCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGc              <  2:240482/91‑1 (MQ=255)                                                             gTGAGTATCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAgc           >  1:858779/1‑91 (MQ=255)                                                             gTGAGTATCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAgc           >  2:422346/1‑91 (MQ=255)                                                              tGAGTATCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAgcg          >  1:238342/1‑91 (MQ=255)                                                                  tATCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAAc      <  2:748618/91‑1 (MQ=255)                                                                  tATCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAAc      <  2:238342/91‑1 (MQ=255)                                                                    tCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAAc      <  2:295965/89‑1 (MQ=255)                                                                    tCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAAc      >  1:295965/1‑89 (MQ=255)                                                                    tCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGa    <  2:858779/91‑1 (MQ=255)                                                                    tCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGa    <  2:362539/91‑1 (MQ=255)                                                                    tCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGa    <  1:482635/91‑1 (MQ=255)                                                                      gTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACa  <  2:549818/91‑1 (MQ=255)                                                                      gTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACa  <  2:308530/91‑1 (MQ=255)                                                                      gTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACa  <  2:154086/91‑1 (MQ=255)                                                                      gTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACa  <  1:615305/91‑1 (MQ=255)                                                                                     |                                                                            GCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACA  >  NZ_CP009273/737815‑737974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑