Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	747,264	T→C	D124G (GAT→GGT)	ybgQ ←	fimbrial biogenesis outer membrane usher protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	747,264	0	T	C	100.0%	111.9 / NA	30	D124G (GAT→GGT)	ybgQ	fimbrial biogenesis outer membrane usher protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/11);  total (19/11)

GGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTT  >  NZ_CP009273/747191‑747347                                                                          |                                                                                    gggAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGACCGAAATTGAAGAGCAtt                                                                    >  2:711301/1‑91 (MQ=255)  ggAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGACCGAAATTGAAGAGCAttt                                                                   >  1:658575/1‑91 (MQ=255)   gAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGACCGAAATTGAAGAGCATTTc                                                                  >  2:684710/1‑91 (MQ=255)   gAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGACCGAAATTGAAGAGCATTTc                                                                  >  1:493022/1‑91 (MQ=255)   gAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGACCGAAATTGAAGAGCATTTc                                                                  >  2:772631/1‑91 (MQ=255)   gAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGGTGCTGATTGGCTTGTCCGAAATTTAAGAGCTTTTc                                                                  >  2:563364/1‑91 (MQ=255)           ttttCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGACCGAAATTGAAGAGCATTTCAGGTCggg                                                          <  1:472403/91‑1 (MQ=255)              tCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGACCGAAATTGAACAGCATTTCAGGTCGGGAAc                                                       <  2:524366/91‑1 (MQ=255)               cTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGACCGAAATTGAAGAGCATTTCAGGTCGGGAACt                                                      >  1:867779/1‑91 (MQ=255)                    tGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGACCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAAt                                                 >  2:466994/1‑91 (MQ=255)                           ccagccagGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGACCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATaca                                          <  2:734618/91‑1 (MQ=255)                            cagccagGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGACCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATacac                                         <  2:38999/91‑1 (MQ=255)                             agccagGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGACCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACAcc                                        >  1:64909/1‑91 (MQ=255)                             agccagGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGACCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACAcc                                        <  1:706657/91‑1 (MQ=255)                                            aTACTAATATTCAGTTGCTGATTGGCTTGACCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCa                         >  2:694438/1‑91 (MQ=255)                                                      tCAGTTGCTGATTGGCTTGACCGAAATTGAAGAGCATTTCAGGTCGGGAACTGaaa                                                  <  1:691684/56‑1 (MQ=255)                                                      tCAGTTGCTGATTGGCTTGACCGAAATTGAAGAGCATTTCAGGTCGGGAACTGaaa                                                  >  2:691684/1‑56 (MQ=255)                                                      tCAGTTGCTGATTGGCTTGACCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATc                                                >  1:328852/1‑58 (MQ=255)                                                      tCAGTTGCTGATTGGCTTGACCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATc                                                <  2:328852/58‑1 (MQ=255)                                                      tCAGTTGCTGATTGGCTTGACCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACg               >  2:692504/1‑91 (MQ=255)                                                      tCAGTTGCTGATTGGCTTGACCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACg               <  1:466994/91‑1 (MQ=255)                                                      tCAGTTGCTGATTGGCTTGACCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACg               >  2:109674/1‑91 (MQ=255)                                                        aGTTGCTGATTGGCTTGACCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGa             <  1:711301/91‑1 (MQ=255)                                                         gTTGCTGATTGGCTTGACCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGat            >  2:279223/1‑91 (MQ=255)                                                         gTTGCTGATTGGCTTGACCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGat            >  2:633700/1‑91 (MQ=255)                                                         gTTGCTGATTGGCTTGACCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGat            >  1:277792/1‑91 (MQ=255)                                                           tGCTGATTGGCTTGACCGAAATTGAAGAGCATTTCAGGTCGGGAACTg                                                     <  1:734331/48‑1 (MQ=255)                                                           tGCTGATTGGCTTGACCGAAATTGAAGAGCATTTCAGGTCGGGAACTg                                                     >  2:734331/1‑48 (MQ=255)                                                            gCTGATTGGCTTGACCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATc         >  2:656764/1‑91 (MQ=255)                                                             cTGATTGGCTTGACCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCt        <  1:724257/91‑1 (MQ=255)                                                                   ggCTTGACCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGtttt  >  1:238377/1‑91 (MQ=255)                                                                          |                                                                                    GGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTT  >  NZ_CP009273/747191‑747347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑