Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	781,531	T→C	I148T (ATC→ACC)	aroG →	3‑deoxy‑7‑phosphoheptulonate synthase AroG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	781,531	0	T	C	100.0%	114.5 / NA	33	I148T (ATC→ACC)	aroG	3‑deoxy‑7‑phosphoheptulonate synthase AroG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/14);  total (19/14)

TCAACGACGGTCTGCGTATAGCCCGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGATCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCACGTACCACCGAATCGCAGGTGCACCGCGAACTG  >  NZ_CP009273/781444‑781613                                                                                        |                                                                                   tCAACGACGGTCTGCGTATAGCCCGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCAc                                                                                 <  1:845665/91‑1 (MQ=255)   aaCGACGGTCTGCGTATAGCCCGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCAccc                                                                               >  1:861692/1‑91 (MQ=255)      gacgGTCTGCGTATAGCCCGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCcac                                                                            <  2:366782/91‑1 (MQ=255)        cgGTCTGCGTATAGCCCGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACaa                                                                          <  1:657713/91‑1 (MQ=255)              gCGTATAGCCCGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATAtctc                                                                    >  1:300529/1‑91 (MQ=255)                gTATAGCCCGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGc                                                                  <  1:538641/91‑1 (MQ=255)                   taGCCCGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGa                                                               <  2:861692/91‑1 (MQ=255)                        cGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCtga                                                          >  1:94270/1‑91 (MQ=255)                        cGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGAGAGATTCCCGCTATGACCACCCCACAATATCTAGCTGACCtga                                                          >  2:217370/1‑91 (MQ=255)                         gTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCtgat                                                         >  1:133345/1‑91 (MQ=255)                         gTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCtgat                                                         >  2:36820/1‑91 (MQ=255)                         gTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCtgat                                                         >  1:343776/1‑91 (MQ=255)                               tgctgcTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTg                                                   <  1:770677/91‑1 (MQ=255)                                gctgctTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCCCAATATCTCGCTGACCTGATGAGCTgg                                                  >  2:30321/1‑91 (MQ=255)                                         tatTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAAtt                                         <  1:710733/91‑1 (MQ=255)                                               cGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCa                                   <  1:36820/91‑1 (MQ=255)                                                  cAGCGGTCTGCCCGCGGCAGGGGGGTTTCTCGATATGACGACCCCCCAATATCCCGCTGCCCTGACGGCCTGGGGCGCAAATTGCGCACGt                                >  2:380409/1‑91 (MQ=255)                                                  cAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGGGCAATTGGCGCACGt                                >  2:164534/1‑91 (MQ=255)                                                  cAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCACGt                                >  2:298371/1‑91 (MQ=255)                                                  cAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCACGt                                <  1:132398/91‑1 (MQ=255)                                                  cAGCGGTCTGCCAGCGGCAGGGGGGTTTCTCGATATGACCACCCCACAGTATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCCCGt                                >  1:665069/1‑91 (MQ=255)                                                    gCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCACGAac                              >  1:862702/1‑91 (MQ=255)                                                    gCGGTCTGCCAGCGGCAGGGGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCACGTac                              >  1:766384/1‑91 (MQ=255)                                                      ggTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGc                                                     <  1:831713/66‑1 (MQ=255)                                                      ggTCTGCCAGCGGCAGGGGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGc                                                     >  2:831713/1‑66 (MQ=255)                                                       gTCTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCACGTaccac                           >  1:471932/1‑91 (MQ=255)                                                         cTGCCAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCACGTACCACCg                         >  1:716361/1‑91 (MQ=255)                                                         cTGCCAGCGGCAGGGGAGGTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGGCCGTACCACCg                         >  2:193361/1‑91 (MQ=255)                                                             cAGCGGCAGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCACGTACCACCGAATc                     <  2:187946/91‑1 (MQ=255)                                                                    aGGTGAGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCACGTACCACCGAATCGCAGGTg              >  2:391446/1‑91 (MQ=255)                                                                         aGTTTCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCACGTACCACCGAATCGCAGGTGCACcg         >  1:570745/1‑91 (MQ=255)                                                                           tttCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCACGTACCACCGAATCGCAGGTGCACcgcg       <  1:673157/91‑1 (MQ=255)                                                                            ttCTCGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCACGTACCACCGAATCGCAGGTGCACCGCGa      >  2:427490/1‑91 (MQ=255)                                                                              ctcGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCACGTACCACCGAATCGCAGGTGCACCGCGAAc    <  2:495154/91‑1 (MQ=255)                                                                                cGATATGACCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCACGTACCACCGAATCGCAGGTGCACCGCGAACTg  <  2:266606/91‑1 (MQ=255)                                                                                        |                                                                                   TCAACGACGGTCTGCGTATAGCCCGTAAATTGCTGCTTGATATTAACGACAGCGGTCTGCCAGCGGCAGGTGAGTTTCTCGATATGATCACCCCACAATATCTCGCTGACCTGATGAGCTGGGGCGCAATTGGCGCACGTACCACCGAATCGCAGGTGCACCGCGAACTG  >  NZ_CP009273/781444‑781613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑