Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	834,220	A→G	D148D (GAT→GAC)	ybiX ←	PKHD‑type hydroxylase YbiX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	834,220	0	A	G	100.0%	112.4 / NA	31	D148D (GAT→GAC)	ybiX	PKHD‑type hydroxylase YbiX
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/14);  total (17/14)

ACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAA  >  NZ_CP009273/834135‑834307                                                                                      |                                                                                        aCTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGcc                                                                                    <  2:187750/91‑1 (MQ=255)   tGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGCTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTg                                                                                  >  1:257886/1‑91 (MQ=255)     gATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGcc                                                                                >  1:119645/1‑91 (MQ=255)     gATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGcc                                                                                >  1:214722/1‑91 (MQ=255)           caTAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGt                                                                          >  1:507170/1‑91 (MQ=255)           caTAAATGATGCCACTCGTACGCCGCGGGTTAAGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTCACGGGAGt                                                                          >  1:97647/1‑91 (MQ=255)                   atgCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCg                                                                  >  2:86713/1‑91 (MQ=255)                    tgCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGa                                                                 >  1:581341/1‑91 (MQ=255)                    tgCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGa                                                                 >  1:104762/1‑91 (MQ=255)                    tgCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGa                                                                 >  1:116499/1‑91 (MQ=255)                       cACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGAtgt                                                              <  1:53065/91‑1 (MQ=255)                        aCTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGAtgtt                                                             >  1:622973/1‑91 (MQ=255)                             tACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCg                                                        >  1:228532/1‑91 (MQ=255)                                   gcgGGTTACGGGTGTCACGCAATGCAGGCTGCTGTAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGGCCGAAGgtg                                                  <  2:49188/91‑1 (MQ=255)                                   gcgGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGgtg                                                  >  1:423210/1‑91 (MQ=255)                                                         tGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTcgccgc                            <  2:257886/91‑1 (MQ=255)                                                               ctgctgGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCcgtcgt                      >  1:320701/1‑91 (MQ=255)                                                               ctgctgGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCcgtcgt                      <  1:490672/91‑1 (MQ=255)                                                                 gctgGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAg                    <  1:267784/91‑1 (MQ=255)                                                                 gctgGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAg                    <  1:546867/91‑1 (MQ=255)                                                                 gctgGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAg                    <  1:718750/91‑1 (MQ=255)                                                                 gctgGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAg                    <  2:440981/91‑1 (MQ=255)                                                                  ctgGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGc                   >  1:338443/1‑91 (MQ=255)                                                                         gATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTgg            >  1:202837/1‑91 (MQ=255)                                                                         gATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTgg            >  1:370110/1‑91 (MQ=255)                                                                            acaacaCGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATc         <  2:19818/91‑1 (MQ=255)                                                                            acaacaCGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATc         <  2:284169/91‑1 (MQ=255)                                                                            acaacaCGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATc         <  2:423210/91‑1 (MQ=255)                                                                            acaacaCGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATc         <  1:668995/91‑1 (MQ=255)                                                                            acaacaCGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTCCGCCGCCGTCGTAGCTTTGTGGATc         <  2:214722/91‑1 (MQ=255)                                                                            acaacaCGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGGTCGCCGCCGTCGTAGCTTTGTGGATc         <  2:663562/91‑1 (MQ=255)                                                                               acacGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCt      >  1:166534/1‑91 (MQ=255)                                                                                  cGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTaa   >  2:588146/1‑91 (MQ=255)                                                                                   gaggTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTaaa  >  2:194270/1‑91 (MQ=255)                                                                                   gaggTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTaaa  >  2:659513/1‑91 (MQ=255)                                                                                      |                                                                                        ACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAA  >  NZ_CP009273/834135‑834307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑