Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	857,539	T→C	E175G (GAA→GGA)	ybiW ←	glycyl radical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	857,539	0	T	C	100.0%	78.0 / NA	22	E175G (GAA→GGA)	ybiW	glycyl radical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/4);  total (18/4)

GCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTTCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTGCCTTCCGCTTTAA  >  NZ_CP009273/857453‑857605                                                                                       |                                                                   gCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCcag                                                                >  1:462256/1‑91 (MQ=255)  cTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCACCAAGCCCTTTTCCcagc                                                               <  1:721874/91‑1 (MQ=255)      ccGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCcagcagca                                                           >  2:112113/1‑91 (MQ=255)                cTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAAtt                                                 >  1:134464/1‑91 (MQ=255)                cTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAAtt                                                 >  2:631915/1‑91 (MQ=255)                cTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAAtt                                                 >  1:430529/1‑91 (MQ=255)                  tCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGTGGGAAATTCa                                               >  1:196424/1‑91 (MQ=255)                  tCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCa                                               >  1:278659/1‑91 (MQ=255)                         ccGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAg                                        >  2:7246/1‑91 (MQ=255)                           gTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGt                                      >  1:522615/1‑91 (MQ=255)                           gTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGt                                      >  2:533499/1‑91 (MQ=255)                                  tGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATc                               >  2:810191/1‑91 (MQ=255)                                   gATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCg                              >  2:786134/1‑91 (MQ=255)                                     tGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGcc                            >  1:457090/1‑91 (MQ=255)                                           agcgaCGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAggc                      >  1:822704/1‑90 (MQ=255)                                           agcgaCGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGt                      <  1:858189/91‑1 (MQ=255)                                                           ttCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTGCCTTCCGCt      <  1:536548/91‑1 (MQ=255)                                                            tCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTgcgc                                  >  1:167388/1‑62 (MQ=255)                                                            tCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTgcgc                                  <  2:167388/62‑1 (MQ=255)                                                            tCCTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTGCCTTCCGCtt     >  2:194121/1‑91 (MQ=255)                                                              cTCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTGCCTTCCGCTTTa   >  2:722016/1‑91 (MQ=255)                                                               tCGCGCAGACCATCAAGCCCTTTTCCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTGCCTTCCGCTTTaa  >  2:84123/1‑91 (MQ=255)                                                                                       |                                                                   GCTCACCGTGTAAATCTTCCAGCACCGTCAGGTTGATGCGCGAGCGACGTTCCGCTACTTCCTCGCGCAGACCATCAAGCCCTTTTTCCAGCAGCAGCGGGAAATTCACCGCCAGGTGCGCATCGCCGGAGGTCATATTGCCTTCCGCTTTAA  >  NZ_CP009273/857453‑857605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑