Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	858,772	T→C	I66V (ATT→GTT)	ybiY ←	glycyl‑radical enzyme activating protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	858,772	0	T	C	100.0%	116.2 / NA	31	I66V (ATT→GTT)	ybiY	glycyl‑radical enzyme activating protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/19);  total (12/19)

GTGTCGGACAGCAGTCGGTTAACGCCGTCAGATGCTCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAGCGCGCGCTCAATCACTTCCGGCGCGGCCTTAGCGCACAGCTCGCAGCCTTCCAGACACAGTCGTGCGTCATACAGCAGATCCTGCGTGCGGGCGC  >  NZ_CP009273/858685‑858855                                                                                        |                                                                                    gtgtCGGACAGCAGTCGGTTAACGCCGTCAGATGCTCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAGCGCGCGCTCAACCAc                                                                                  >  1:196604/1‑91 (MQ=255) gtgtCGGACAGCAGTCGGTTAACGCCGTCAGATGCTCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAGCGCGCGCTCAACCAc                                                                                  >  2:716535/1‑91 (MQ=255) gtgtCGGACAGCAGTCGGTTAACGCCGTCAGATGCTCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAGCGCGCGCTCAACCAc                                                                                  >  2:198065/1‑91 (MQ=255) gtggCGGACAGCGGTCGGTTAACGCCGTCAGATGCTCCGGGGTTAACTCTTCCCGATGAATAAGCAAACCATTCAGCGCGCGCTCAACCAc                                                                                  >  1:813518/1‑91 (MQ=255)         cagcagTCGGTTAACGCCGTCAGATGCTCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAGCGCGCGCTCAACCACTTCCGgcg                                                                          <  2:442593/91‑1 (MQ=255)         cagcagTCGGTTAACGCCGTCAGATGCTCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAGCGCGCGCTCAACCACTTCCGgcg                                                                          <  2:229886/91‑1 (MQ=255)          agcagTCGGTTAACGCCGTCAGATGCTCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAGCGCGCGCTCAACCACTTCCGgcgc                                                                         <  2:512232/91‑1 (MQ=255)          agcagTCGGTTAACGCCGTCAGATGCTCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAGCGCGCGCTCAACCACTTCCGgcgc                                                                         <  2:374816/91‑1 (MQ=255)          agcagTCGGTTAACGCCGTCAGATGCTCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAGCGCGCGCTCAACCACTTCCGgcgc                                                                         <  1:96752/91‑1 (MQ=255)           gcagTCGGTTAACGCCGTCAGATGCTCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAGCGCGCGCTCAACCACTTCCGGcgcg                                                                        >  1:213454/1‑91 (MQ=255)                   ttAACGCCGTCAGATGCTCCGGGGTTAACTTTTCCCCATGAATAAGCAAACCATTCAGCGCGCGCTCAACCACTTCCGGCGCGGCCTTAgc                                                                >  2:561235/1‑91 (MQ=255)                                aTGCTCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAGCGCGCGCTCAACCACTTCCGGCGCGGCCTTAGCGCACAGCTCGc                                                     <  2:706677/89‑1 (MQ=255)                                aTGCTCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAGCGCGCGCTCAACCACTTCCGGCGCGGCCTTAGCGCACAGCTCGc                                                     >  1:706677/1‑89 (MQ=255)                                aTGCTCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAGCGCGCGCTCAACCACTTCCGGCGCGGCCTTAGCGCACAGCTCGCAg                                                   <  2:182491/91‑1 (MQ=255)                                aTGCTCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAGCGCGCGCTCAACCACTTCCGGCGCGGCCTTAGCGCACAGCTCGCAg                                                   <  2:229728/91‑1 (MQ=255)                                aTGCTCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAGCGCGCGCTCAACCACTTCCGGCGCGGCCTTAGCGCACAGCTCGCAg                                                   <  1:314476/91‑1 (MQ=255)                                aTGCTCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAGCGCGCGCTCAACCACTTCCGGCGCGGCCTTAGCGCACAGCTCGCAg                                                   <  1:716535/91‑1 (MQ=255)                                     ccGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAGCGCGCGCTCAACCACTTCCGGCGCGGCCTTAGCGCACAGCTCGCAGCCTTc                                              >  2:169939/1‑91 (MQ=255)                                                 tttCCCGATGAATAAGCAAACCATTCAGCGCGCGCTCAACCACTTCCGGCGCGGCCTTAGCGCACAGCTCGCAGCCTTCCAGACACAGTCg                                  <  2:176564/91‑1 (MQ=255)                                                 tttCCCGATGAATAAGCAAACCATTCAGCGCGCGCTCAACCACTTCCGGCGCGGCCTTAGCGCACAGCTCGCAGCCTTCCAGACACAGTCg                                  <  2:815767/91‑1 (MQ=255)                                                    cccGATGAATAAGCAAACCATTCGGCGCGCGCTCAACCACTTCCGGCGCGGCCTTAGCGCACAGCTCGCAGCCTTCCAGACACAGTCGTGc                               <  2:342238/91‑1 (MQ=255)                                                    cccGATGAATAAGCAAACCATTCAGCGCGCGCTCAACCACTTCCGGCGCGGCCTTAGCGCACAGCTCGCAGCCTTCCAGACACAGTCGTGc                               <  1:10781/91‑1 (MQ=255)                                                    cccGATGAATAAGCAAACCATTCAGCGCGCGCTCAACCACTTCCGGCGCGGCCTTAGCGCACAGCTCGCAGCCTTCCAGACACAGTCGTGc                               <  1:33124/91‑1 (MQ=255)                                                                    aCCATTCAGCGCGCGCTCAACCACTTCCGGCGCGGCCTTAGCGCACAGCTCGCAGCCTTCCAGACACAGTCGTGCGTCATACAGCAGATcc               <  2:202796/91‑1 (MQ=255)                                                                      cATTCAGCGCGCGCTCAACCACTTCCGGCGCGGCCTTAGCGCACAGCTCGCAGCCTTCCAGACACAGTCGTGCGTCATACAGCAGATCCtg             >  2:661758/1‑91 (MQ=255)                                                                            gcgcgcgcTCAACCACTTCCGGCGCGGCCTTAGCGCACAGCTCGCAg                                                   >  1:787340/1‑47 (MQ=255)                                                                            gcgcgcgcTCAACCACTTCCGGCGCGGCCTTAGCGCACAGCTCGCAg                                                   <  2:787340/47‑1 (MQ=255)                                                                              gcgcgcTCAACCACTTCCGGCGCGGCCTTAGCGCACAGCTCGCAGCCTTCCAGACACAGTCGTGc                               >  1:562213/1‑65 (MQ=255)                                                                              gcgcgcTCAACCACTTCCGGCGCGGCCTTAGCGCACAGCTCGCAGCCTTCCAGACACAGTCGTGc                               <  2:562213/65‑1 (MQ=255)                                                                               cgcgcTCAACCACTTCCGGCGCGGCCTTAGCGCACAGCTCGCAGCCTTCCAGACACAGTCGTGCGTCATa                         <  2:426211/70‑1 (MQ=255)                                                                               cgcgcTCAACCACTTCCGGCGCGGCCTTAGCGCACAGCTCGCAGCCTTCCAGACACAGTCGTGCGTCATa                         >  1:426211/1‑70 (MQ=255)                                                                                 cgcTCAACCACTTCCGGCGCGGCCTTAGCGCACAGCTCGCAGCCTTCCAGACACAGTCGTGCGTCATACAGCAGATCCTGCGTGCGGgcgc  <  1:537557/91‑1 (MQ=255)                                                                                 cgcTCAACCACTTCCGGCGCGGCCTTAGCGCACAGCTCGCAGCCTTCCAGACACAGTCGTGCGTCATACAGCAGATCCTGCGTGCGGgcgc  >  1:322618/1‑91 (MQ=255)                                                                                        |                                                                                    GTGTCGGACAGCAGTCGGTTAACGCCGTCAGATGCTCCGGGGTTAACTTTTCCCGATGAATAAGCAAACCATTCAGCGCGCGCTCAATCACTTCCGGCGCGGCCTTAGCGCACAGCTCGCAGCCTTCCAGACACAGTCGTGCGTCATACAGCAGATCCTGCGTGCGGGCGC  >  NZ_CP009273/858685‑858855

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑