Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	861,316	T→C	I169V (ATT→GTT)	moeA ←	molybdopterin molybdotransferase MoeA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	861,316	0	T	C	100.0%	67.1 / NA	19	I169V (ATT→GTT)	moeA	molybdopterin molybdotransferase MoeA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (5/14);  total (5/14)

TCGCCCAGCGGCTGACCGGGCAACTGGAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAATCCCCAGTGAAGCAATTACTGGCAGCTCTGCGGTAGTCAGGCGAGTTCCCGCCGGGAAAACAACCGCACCTGCAGAGATA  >  NZ_CP009273/861228‑861395                                                                                         |                                                                                tCGCCCAGCGGCTGACCGGGCAACTGGAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAAccc                                                                               >  2:80354/1‑91 (MQ=255)   gCCCAGCGGCTGACCGGGCAACTGGAGTTCATCACCGGTAGACAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAACcccc                                                                             <  1:814609/91‑1 (MQ=255)   gCCCAGCGGCTGACCGGGCAACTGGAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAACcccc                                                                             <  1:584515/91‑1 (MQ=255)   gCCCAGCGGCTGACCGGGCAACTGGAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAACcccc                                                                             <  2:187243/91‑1 (MQ=255)         cGGCTGACCGGGCAACTGGAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAACCCCCAGTGaa                                                                       <  2:226112/91‑1 (MQ=255)             tGACCGGGCAACTGGAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAACCCCCAGTGAAGCaa                                                                   >  2:118548/1‑91 (MQ=255)                     cAACTGGAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAACCCCCAGTGAAGCAATTACTGGc                                                           <  2:592104/91‑1 (MQ=255)                       aCTGGAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAACCCCCAGTGAAGCAATTACTGGCAg                                                         >  2:223773/1‑91 (MQ=255)                        cTGGAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAACCCCCAGTGAAGCAATTACTGGCAGc                                                        <  1:223773/91‑1 (MQ=255)                        cTGGAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAACCCCCAGTGAAGCAATTACTGGCAGc                                                        <  1:358798/91‑1 (MQ=255)                                 atcaCCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAACCCCCAGTGAAGCAATTACTGGCAGCTCTGCGGTa                                               >  2:43875/1‑91 (MQ=255)                                       ggTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAACCCCCAGTGAAGCAATTACTGGCAGCTCTGCGGTAGTCAgg                                         >  2:325149/1‑91 (MQ=255)                                                               tttACGAATCACCGGAACTTCGGCAACCCCCAGTGAAGCAATTACTGGCAGCTCTGCGGTAGTCAGGCGAGTTCCCGCCGGGAAAACAAcc                 <  1:766912/91‑1 (MQ=255)                                                               tttACGAATCACCGGAACTTCGGCAACCCCCAGTGAAGCAATTACTGGCAGCTCTGCGGTAGTCAGGCGAGTTCCCGCCGGGAAAACAAcc                 <  1:325149/91‑1 (MQ=255)                                                                       tCACCGGAACTTCGGCAACCCCCAGTGAAGCAATTACTGGCAGCTCTGCGGTAGTCAGGCGAGTTCCCGCCGGGAAAACAACCGCACCTGc         <  1:429009/91‑1 (MQ=255)                                                                        cACCGGAACTTCGGCAACCCCCAGTGAAGCAATTACTGGCAGCTCTGCGGTAGTCAGGCGAGTTCCCGCCGGGAAAACAACCGCACCTGCa        >  1:157533/1‑91 (MQ=255)                                                                           cGGAACTTCGGCAACCCCCAGTGAAGCAATTACTGGCAGCTCTGCGGTAGTCAGGCGAGTTCCCGCCGGGAAAACAACCGCACCTGCagag     <  1:468722/91‑1 (MQ=255)                                                                           cGGAACTTCGGCAACCCCCAGTGAAGCAATTACTGGCAGCTCTGCGGTAGTCAGGCGAGTTCCCGCCGGGAAAACAACCGCACCTGCagag     <  1:230459/91‑1 (MQ=255)                                                                             gAACTTCGGCAACCCCCAGTGAAGCAATTACTGGCAGCTCTGCGGTAGTCAGGCGAGTTCCCGCCGGGAAAACAACCGCACCTGCAGAGat   <  1:475759/91‑1 (MQ=255)                                                                              aaCTTCGGCAACCCCCAGTGAAGCAATTACTGGCAGCTCTGCGGTAGTCAGGCGAGTTCCCGCCGGGAAAACAACCGCACCTGCAGAGata  <  1:112383/91‑1 (MQ=255)                                                                                         |                                                                                TCGCCCAGCGGCTGACCGGGCAACTGGAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAATCCCCAGTGAAGCAATTACTGGCAGCTCTGCGGTAGTCAGGCGAGTTCCCGCCGGGAAAACAACCGCACCTGCAGAGATA  >  NZ_CP009273/861228‑861395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑