Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	900,176	T→G	V43G (GTT→GGT)	ybjQ →	heavy metal‑binding domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	900,176	0	T	G	100.0%	70.4 / NA	20	V43G (GTT→GGT)	ybjQ	heavy metal‑binding domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (9/11);  total (9/11)

TGAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAGGCTCCCAGG  >  NZ_CP009273/900093‑900253                                                                                    |                                                                              tGAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGa                                                                        <  2:225730/91‑1 (MQ=255)  gAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGAc                                                                       >  1:223797/1‑91 (MQ=255)  gAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGAc                                                                       >  1:236529/1‑91 (MQ=255)            gtgtGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGc                                                             >  1:695148/1‑91 (MQ=255)              gtggtgACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGt                                                           >  1:673915/1‑91 (MQ=255)                ggtgACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTAt                                                         <  1:714143/91‑1 (MQ=255)                          gAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAAc                                               <  1:729651/91‑1 (MQ=255)                          gAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAAc                                               <  2:199040/91‑1 (MQ=255)                               gATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGt                                          <  2:558574/91‑1 (MQ=255)                               gATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGt                                          <  2:685010/91‑1 (MQ=255)                                 ttttAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTaa                                        <  1:548552/91‑1 (MQ=255)                                       gTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACg                                  <  2:515150/91‑1 (MQ=255)                                          ccAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGga                               >  1:442153/1‑91 (MQ=255)                                          ccAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGga                               >  2:626143/1‑91 (MQ=255)                                              tatTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATc                           <  1:333934/91‑1 (MQ=255)                                              tatTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATc                           <  1:330876/91‑1 (MQ=255)                                                     cGTGATTTCTTTGCCGGTATCCGCGATATCGGTGGCGGACGTTCCGCTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTg                    >  1:844704/1‑91 (MQ=255)                                                                       aTCCGCGATATCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAGGCTCCCAgg  >  2:295357/1‑91 (MQ=255)                                                                               tatCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTaa                                        >  1:177589/1‑45 (MQ=255)                                                                               tatCGGTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTaa                                        <  2:177589/45‑1 (MQ=255)                                                                                    |                                                                              TGAATATTGCGGTGTGGTGACCGGCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAATTAGGCTCCCAGG  >  NZ_CP009273/900093‑900253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑