Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	923,801	A→G	V284A (GTC→GCC)	cydC ←	cysteine/glutathione ABC transporter ATP‑binding protein/permease CydC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	923,801	0	A	G	100.0%	129.0 / NA	34	V284A (GTC→GCC)	cydC	cysteine/glutathione ABC transporter ATP‑binding protein/permease CydC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/15);  total (19/15)

GAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGACAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAGCATCAGGATCACCGCTAAC  >  NZ_CP009273/923723‑923884                                                                               |                                                                                    gagGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCaa                                                                         >  2:531370/1‑91 (MQ=255) gagGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCaa                                                                         >  2:760837/1‑91 (MQ=255)     cAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCTAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTaa                                                                     <  1:331110/91‑1 (MQ=255)          aCTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACgcgc                                                                <  2:613243/91‑1 (MQ=255)          aCTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACgcgc                                                                <  1:465093/91‑1 (MQ=255)             tGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCgg                                                             >  2:756672/1‑91 (MQ=255)                  cAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGa                                                        >  1:259404/1‑91 (MQ=255)                     aTGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCa                                                     >  1:268452/1‑91 (MQ=255)                      tGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCAt                                                    <  1:452466/91‑1 (MQ=255)                      tGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCAt                                                    <  1:215098/91‑1 (MQ=255)                              tGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCa                                            >  1:377998/1‑91 (MQ=255)                              tGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCa                                            <  1:577843/91‑1 (MQ=255)                               gCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCaa                                           >  2:766199/1‑91 (MQ=255)                                 aCCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAAcg                                         >  2:515894/1‑91 (MQ=255)                                            gTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGc                              <  2:608606/91‑1 (MQ=255)                                              gCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCa                                                                          <  1:559841/45‑1 (MQ=255)                                              gCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCa                                                                          >  2:559841/1‑45 (MQ=255)                                              gCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCa                            >  1:781858/1‑91 (MQ=255)                                                cAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCAt                           <  2:856413/90‑1 (MQ=255)                                                cAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCAt                           >  1:856413/1‑90 (MQ=255)                                                 aGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATcc                         >  2:693581/1‑91 (MQ=255)                                                  gTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCca                        >  1:808219/1‑91 (MQ=255)                                                  gTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCca                        >  1:735333/1‑91 (MQ=255)                                                   tGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCcac                       <  2:268452/91‑1 (MQ=255)                                                   tGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCcac                       <  1:49648/91‑1 (MQ=255)                                                     cTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAg                     <  2:701258/91‑1 (MQ=255)                                                     cTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAg                     <  2:775171/91‑1 (MQ=255)                                                             gcgGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAGCATCAGGa             <  2:456128/91‑1 (MQ=255)                                                               ggCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAGCATCAGGATc           >  2:138270/1‑91 (MQ=255)                                                               ggCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAGCATCAGGATc           >  2:741263/1‑91 (MQ=255)                                                               ggCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAGCATCAGGATc           >  1:349606/1‑91 (MQ=255)                                                                        gcAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGc                              <  2:262610/63‑1 (MQ=255)                                                                        gcAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGc                              >  1:262610/1‑63 (MQ=255)                                                                        gcAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAGCATCAGGATCACCGCTAAc  >  2:708036/1‑91 (MQ=255)                                                                               |                                                                                    GAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGACAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAGCATCAGGATCACCGCTAAC  >  NZ_CP009273/923723‑923884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑