Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	974,173	T→C	S798P (TCC→CCC)	mukB →	chromosome partition protein MukB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	974,173	0	T	C	100.0%	125.5 / NA	33	S798P (TCC→CCC)	mukB	chromosome partition protein MukB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/20);  total (13/20)

TATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTTCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGTTCAGCCGCTTTATCGGC  >  NZ_CP009273/974086‑974250                                                                                        |                                                                              tATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCg                                                                            >  1:435688/1‑91 (MQ=255)    tCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAAc                                                                         >  2:747051/1‑91 (MQ=255)      aCGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGc                                                                       <  2:455684/91‑1 (MQ=255)       cGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCt                                                                      >  2:455858/1‑91 (MQ=255)       cGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCt                                                                      >  2:259248/1‑91 (MQ=255)       cGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCt                                                                      >  1:684221/1‑91 (MQ=255)           tCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGc                                                                  <  2:711350/91‑1 (MQ=255)              cGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCAc                                                               <  2:85985/91‑1 (MQ=255)              cGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCAc                                                               <  1:459667/91‑1 (MQ=255)                gAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGc                                                             <  2:703489/91‑1 (MQ=255)                gAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGc                                                             <  1:761750/91‑1 (MQ=255)                gAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGc                                                             <  2:296914/91‑1 (MQ=255)                gAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGc                                                             <  2:684221/91‑1 (MQ=255)                  aGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGctc                                                           <  2:243490/91‑1 (MQ=255)                  aGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGctc                                                           <  1:35702/91‑1 (MQ=255)                     gCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTcc                                                        <  2:687663/91‑1 (MQ=255)                     gCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTcc                                                        <  1:377735/91‑1 (MQ=255)                       cGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCtt                                                      <  1:118329/91‑1 (MQ=255)                              tGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTa                                               <  1:424222/91‑1 (MQ=255)                                gTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACa                                             >  1:571635/1‑91 (MQ=255)                                   gTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGctc                                                           <  2:273842/74‑1 (MQ=255)                                   gTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGctc                                                           >  1:273842/1‑74 (MQ=255)                                     gctgcGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGaaaa                                        >  2:350209/1‑91 (MQ=255)                                         cgcgTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCa                                    <  2:343257/91‑1 (MQ=255)                                                       aTTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAgg                      <  2:571635/91‑1 (MQ=255)                                                               ccTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGTTCAGc              >  2:459408/1‑91 (MQ=255)                                                                cTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGTTCAGcc             <  2:601524/91‑1 (MQ=255)                                                                 tCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGTTCAGCCg            <  2:535467/91‑1 (MQ=255)                                                                 tCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGTTCAGCCg            >  1:639013/1‑91 (MQ=255)                                                                        cGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCTGGCGTTCAGCCGCTTTATc     >  1:297060/1‑91 (MQ=255)                                                                        cGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAAAGTCTGCATCAGGCGTTCAGCCGCTTTATc     >  2:783573/1‑91 (MQ=255)                                                                           gCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGTTCAGCCGCTTTATCGGc  <  1:310170/91‑1 (MQ=255)                                                                           gCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCGGCATCACGAGTTCAGCCGCTTTAtgtgc  >  2:201905/1‑87 (MQ=255)                                                                                        |                                                                              TATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTTCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGTTCAGCCGCTTTATCGGC  >  NZ_CP009273/974086‑974250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑