Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	152,657	A→G	L11S (TTA→TCA)	yadV ←	fimbrial chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	152,657	0	A	G	100.0%	141.0 / NA	37	L11S (TTA→TCA)	yadV	fimbrial chaperone
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/25);  total (12/25)

TGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAGGGAATAATATAAAATAGAAAT  >  NZ_CP009273/152570‑152740                                                                                        |                                                                                    tGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATGAAg                                                                                  <  2:257249/91‑1 (MQ=255)  gATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATGAAGc                                                                                 <  1:174045/91‑1 (MQ=255)  gATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATGAAGc                                                                                 <  2:744382/91‑1 (MQ=255)      gCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATGAAGCtgtt                                                                             <  2:538649/91‑1 (MQ=255)        ttttATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATGAAGCtgttgt                                                                           <  1:468905/91‑1 (MQ=255)                 ttACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATGAAGc                                                                                 <  2:813542/76‑1 (MQ=255)                 ttACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATGAAGc                                                                                 >  1:813542/1‑76 (MQ=255)                 ttACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATGAAGCTGTTGTATGTTTGgt                                                                  >  2:91652/1‑91 (MQ=255)                 ttACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATGAAGCTGTTGTATGTTTGgt                                                                  <  2:775294/91‑1 (MQ=255)                   aCGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATGAAg                                                                                  <  1:37860/73‑1 (MQ=255)                   aCGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATGAAg                                                                                  >  2:37860/1‑73 (MQ=255)                   aCGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATGAAGCTGTTGTATGTTTGgtgt                                                                >  2:448036/1‑91 (MQ=255)                      cgAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATGAAGCTGTTGTATGTTTGGTGTTaa                                                             >  1:161237/1‑91 (MQ=255)                      cgAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATGAAGCTGTTGTATGTTTGGTGTTaa                                                             >  1:475276/1‑91 (MQ=255)                       gAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATGAAGc                                                                                 <  1:547276/70‑1 (MQ=255)                       gAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATGAAGc                                                                                 >  2:547276/1‑70 (MQ=255)                       gAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATGAAGCTGTTGTATGTTTGGTGTTaaa                                                            <  2:155216/91‑1 (MQ=255)                             ccGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATGAAGCTGTTGTATGTTTGGTGTTAAAAAACAt                                                      <  2:396708/91‑1 (MQ=255)                                            ccGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATGAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATa                                       >  1:711697/1‑91 (MQ=255)                                                aaTAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATGAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGt                                   <  1:829586/91‑1 (MQ=255)                                                aaTAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATGAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGt                                   <  2:699028/91‑1 (MQ=255)                                                  tAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATGAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGtta                                 <  2:475276/91‑1 (MQ=255)                                                      cGATGAACTAAAAGCCATACAGGTTACGAAGCATGAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGttaatta                             >  1:124974/1‑91 (MQ=255)                                                            aCTAAAAGCCATACAGGTTACGAAGCATGAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAg                       <  2:516189/91‑1 (MQ=255)                                                            aCTAAAAGCCATACAGGTTACGAAGCATGAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAg                       <  1:848910/91‑1 (MQ=255)                                                            aCTAAAAGCCATACAGGTTACGAAGCATGAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAg                       <  2:711697/91‑1 (MQ=255)                                                                 aaGCCATACAGGTTACGAAGCATGAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAGGGaat                  <  2:723279/91‑1 (MQ=255)                                                                 aaGCCATACAGGTTACGAAGCATGAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAGGGaat                  <  1:544010/91‑1 (MQ=255)                                                                      aTACAGGTTACGAAGCATGAAGCTGTTGTATGTTTGGTGTTAAAAAACAt                                                      <  2:621743/50‑1 (MQ=255)                                                                      aTACAGGTTACGAAGCATGAAGCTGTTGTATGTTTGGTGTTAAAAAACAt                                                      >  1:621743/1‑50 (MQ=255)                                                                      aTACAGGTTACGAAGCATGAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAGAGAATAatat             >  1:271973/1‑91 (MQ=255)                                                                               aCGAAGCATGAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAGGGAATAATATAAAATAGaa    <  2:341843/91‑1 (MQ=255)                                                                               aCGAAGCATGAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAGGGAATAATATAAAATAGaa    <  1:91652/91‑1 (MQ=255)                                                                               aCGAAGCATGAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAGGGAATAATATAAAATAGaa    <  2:849555/91‑1 (MQ=255)                                                                                 gAAGCATGAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAGGGAATAATATAAAATAGAAat  <  1:448036/91‑1 (MQ=255)                                                                                    gCATGAAGCTGTTGTATGTTTGGTGTTAAAAAACAt                                                      <  2:683929/36‑1 (MQ=255)                                                                                    gCATGAAGCTGTTGTATGTTTGGTGTTAAAAAACAt                                                      >  1:683929/1‑36 (MQ=255)                                                                                        |                                                                                    TGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAGGGAATAATATAAAATAGAAAT  >  NZ_CP009273/152570‑152740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑