BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	987,566	A→T	I497F (ATC→TTC)	pepN →	aminopeptidase N

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	987,566	0	A	T	100.0%	81.4 / NA	24	I497F (ATC→TTC)	pepN	aminopeptidase N
Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (11/13); total (11/13)

ACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGCGGAACAGACCTTTGTCTTTGATAATGTCTA  >  NZ_CP009273/987482‑987651

aCGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTACGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGTTCCCGt                                                                                 >  1:668936/1‑91 (MQ=255)

           gCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGTTCCCGTTGCAGAAAGGc                                                                      >  2:511/1‑91 (MQ=255)

               gATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGTTCCCGTTGCAGAAAGGCGGTc                                                                  >  2:444855/1‑91 (MQ=255)

                  cAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGTTCCCGTTGCAGAAAGGCGGTCATc                                                               <  2:83893/91‑1 (MQ=255)

                  cAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGTTCCCGTTGCAGAAAGGCGGTCATc                                                               <  1:563633/91‑1 (MQ=255)

                        gAAAAACAGCCGCTGCATGTTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGTTCCCGTTGCAGAAAGGCGGTCATCCGGTGa                                                         >  1:545890/1‑91 (MQ=255)

                              cAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGTTCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCg                                                   <  1:399262/91‑1 (MQ=255)

                                      gCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGTTCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAAc                                           <  2:37639/91‑1 (MQ=255)

                                                 ttGCCATCGAACTGTATGATAACGAAGGCAAAGTGTTCCCGTTGTAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGc                                <  1:545232/91‑1 (MQ=255)

                                                 ttGCCATCGAACTGTATGATAACGAAGGCAAAGTGTTCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGc                                <  2:133934/91‑1 (MQ=255)

                                                 ttGCCAGCGAACTGTATGATAACGAAGGCAAAGTGTTCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGc                                <  2:143409/91‑1 (MQ=255)

                                                     cATCGAACTGTATGATAACGAAGGCAAAGCGTTCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGACCGTCACTCAGGCGGaa                            >  2:651516/1‑91 (MQ=255)

                                                             tGTATGATAACGAAGGCAAAGTGTTCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGCGGAACAGACCtt                    <  1:274214/91‑1 (MQ=255)

                                                             tGTATGATAACGAAGGCAAAGTGTTCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGCGGAACAGACCtt                    <  1:821709/91‑1 (MQ=255)

                                                                 tGATAACGAAGGCAAAGTGTTCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGCGGAACAGACCTTTGTc                >  1:450422/1‑91 (MQ=255)

                                                                    tAACGAAGGCAAAGTGTTCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTACGTGCTGACAGTCACTCAGGCGGAACAGACCTTTGTCttt             >  2:650534/1‑91 (MQ=255)

                                                                        gAAGGCAAAGTGTTCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGCGGAACAGACCTTTGTCTTTGATa         >  2:172326/1‑91 (MQ=255)

                                                                        gAAGGCAAAGTGTTCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGCGGAACAGACCTTTGTCTTTGATa         >  2:127293/1‑91 (MQ=255)

                                                                         aaGGCAAAGTGTTCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGCGGAACAGACCTTTGTCTTTGATaa        <  2:483823/91‑1 (MQ=255)

                                                                          aGGCAAAGTGTTCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTc                                        >  2:32065/1‑58 (MQ=255)

                                                                          aGGCAAAGTGTTCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTc                                        <  1:32065/58‑1 (MQ=255)

                                                                             cAAAGTGTTCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGCGGAACAGACCTTTGTCTTTGATAATGTc    <  2:852410/91‑1 (MQ=255)

                                                                              aaaGTGTTCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGCGGAACAGACCTTTGTCTTTGATAATGTCt   <  2:31600/91‑1 (MQ=255)

                                                                               aaGTGTTCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGCGGAACAGACCTTTGTCTTTGATAATGTCTa  >  1:270226/1‑91 (MQ=255)

ACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGCGGAACAGACCTTTGTCTTTGATAATGTCTA  >  NZ_CP009273/987482‑987651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`