Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,124,635	T→C	R447R (CGT→CGC)	murJ →	murein biosynthesis integral membrane protein MurJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,124,635	0	T	C	100.0%	116.1 / NA	32	R447R (CGT→CGC)	murJ	murein biosynthesis integral membrane protein MurJ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/25);  total (7/25)

CGTGTCTGAATGCTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCATTGGGTACCATGCCC  >  NZ_CP009273/1124547‑1124719                                                                                         |                                                                                     cgTGTCTGAATGCTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCt                                                                                    <  1:379454/91‑1 (MQ=255)  gtgtCTGAATGCTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCtg                                                                                   >  2:454395/1‑91 (MQ=255)          aTGCTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGc                                                                           <  1:54445/91‑1 (MQ=255)          aTGCTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGc                                                                           <  1:569378/91‑1 (MQ=255)               tCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTAc                                                                      <  2:539915/91‑1 (MQ=255)                    gctTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTg                                                                 <  2:749571/91‑1 (MQ=255)                              cAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCg                                                       >  1:349628/1‑91 (MQ=255)                              cAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCg                                                       <  2:492135/91‑1 (MQ=255)                              cAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCg                                                       <  2:349628/91‑1 (MQ=255)                               aGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGt                                                      <  2:284961/91‑1 (MQ=255)                                          cAGAAAATCTTTACCCCGCACCTCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGTCGGTACTGGTGATGTCTGGCCGGCTTTTAggga                                           <  2:440953/91‑3 (MQ=255)                                          cAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTa                                           <  1:620017/91‑1 (MQ=255)                                          cAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTa                                           <  2:390159/91‑1 (MQ=255)                                          cAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTa                                           <  2:232810/91‑1 (MQ=255)                                          cAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTa                                           <  2:229581/91‑1 (MQ=255)                                          cAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTa                                           <  1:147659/91‑1 (MQ=255)                                             aaaaTCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGGTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGt                                        <  1:376760/91‑1 (MQ=255)                                                       ccccGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCAt                              <  1:297738/91‑1 (MQ=255)                                                       ccccGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCAt                              <  2:838502/91‑1 (MQ=255)                                                          cGCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGcc                           >  2:533408/1‑91 (MQ=255)                                                           gCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCg                          >  2:758614/1‑91 (MQ=255)                                                           gCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCg                          >  1:427192/1‑91 (MQ=255)                                                           gCAACCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCg                          >  2:559615/1‑91 (MQ=255)                                                              aCCCGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAg                       <  1:454395/91‑1 (MQ=255)                                                               cccGGCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGt                      <  2:607585/91‑1 (MQ=255)                                                                  ggCTGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGt                   <  1:519410/91‑1 (MQ=255)                                                                     tGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCAt                <  1:533408/91‑1 (MQ=255)                                                                     tGGATGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCAt                <  1:448502/91‑1 (MQ=255)                                                                         tGGCGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTGGGTATGTTACATATCATGCCGGAGTGGTCATTggg            <  1:473621/91‑1 (MQ=255)                                                                            cGTTTCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCATTGGGTAc         <  1:777807/91‑1 (MQ=255)                                                                                tCTGTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCATTGGGTACCATg     >  2:622982/1‑91 (MQ=255)                                                                                   gTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCATTGGGTACCATGccc  <  1:468324/91‑1 (MQ=255)                                                                                   gTTGCGCCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCATTGGGTACCATGccc  <  1:293918/91‑1 (MQ=255)                                                                                         |                                                                                     CGTGTCTGAATGCTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCATTGGGTACCATGCCC  >  NZ_CP009273/1124547‑1124719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑