| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,152,873 | A→T | H214L (CAT→CTT) | ycfH → | metal‑dependent hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,152,873 | 0 | A | T | 97.4% | 143.6 / NA | 38 | H214L (CAT→CTT) | ycfH | metal‑dependent hydrolase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base T (22/15); minor base G (0/1); total (22/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGGAGCAACTGCGCGATGCTGCGCGTTATGTCCCCCTGGATCGGTTACTGGTGGAAACTGACTCACCTTACCTTGCGCCGGTACCGCATCGAGGAAAAGAGAATCAACCTGCGATGGTTCGTGACGTTGCAGAATACATGGCTGTGTTGAAAGGTGTTGCCGTTG > NZ_CP009273/1152786‑1152950 | cGGAGCAACTGCGCGATGCTGCGCGTTATGTCCCCCTGGATCGGTTACTGGTGGAAACTGACTCACCTTACCTGGCGCCGGTACCGCGTCg < 2:2657/91‑1 (MQ=255) ggAGCAACTGCGCGATGCTGCGCGTTATGTCCCCCTGGATCGGTTACTGGTGGAAACTGACTCACCTTACCTTGCGCCGGTACCGCTTCGa > 1:47701/1‑91 (MQ=255) gAGCAACTGCGCGATGCTGCGCGTTATGTCCCCCTGGATCGGTTACTGGTGGAAACTGACTCACCTTACCTTGCGCCGGTACCGCTTCGAg > 2:10761/1‑91 (MQ=255) gCAACTGCGCGATGCTGCGCGTTATGTCCCCCTGGATCGGTTACTGGTGGAAACTGACTCACCTTACCTTGCGCCGGTACCGCTTCGAGGa < 1:474470/91‑1 (MQ=255) tGCGCGATGCTGCGCGTTATGTCCCCCTGGATCGGTTACTGGTGGAAACTGACTCACCTTACCTTGCGCCGGTACCGCTTCGAGGAAAaga > 1:396893/1‑91 (MQ=255) gcgATGCTGCGCGTTATGTCCCCCTGGATCGGTTACTGGTGGAAACTGACTCACCTTACCTTGCGCCGGTACCGCTTCGAGGAAAAGAGaa > 1:391483/1‑91 (MQ=255) cgTTATGTCCCCCTGGATCGGTTACTGGTGGAAACTGACTCACCTTACCTTGCGCCGGTACCGCTTCGAGGAAAAGAGAATCAACCTGCGa < 2:769025/91‑1 (MQ=255) cgTTATGTCCCCCTGGATCGGTTACTGGTGGAAACTGACTCACCTTACCTTGCGCCGGTACCGCTTCGAGGAAAAGAGAATCAACCTGCGa < 1:661255/91‑1 (MQ=255) aTGTCCCCCTGGATCGGTTACTGGTGGAAACTGACTCACCTTACCTTGCGCCGGTACCGCTTCGAGGAAAAGAGAATCAACCTGCGATGGt > 2:227150/1‑91 (MQ=255) gTCCCCCTGGATCGGTTACTGGTGGAAACTGACTCACCTTACCTTGCGCCGGTACCGCTTCGAGGAAAAGAGAATCAACCTGCGATGGTTc > 2:257697/1‑91 (MQ=255) cccccTGGATCGGTTACTGGTGGAAACTGACTCACCTTACCTTGCGCCGGTACCGCTTCGAGGAAAAGAGAATCAACCTGCGATGGTTCGt < 1:233362/91‑1 (MQ=255) ccccTGGATCGGTTACTGGTGGAAACTGACTCACCTTACCTTGCGCCGGTACCGCTTCGAGGAAAAGAGAATCAACCTGCGATGGTTCGTg > 1:483411/1‑91 (MQ=255) ccccTGGATCGGTTACTGGTGGAAACTGACTCACCTTACCTTGCGCCGGTACCGCTTCGAGGAAAAGAGAATCAACCTGCGATGGTTCGTg > 1:687192/1‑91 (MQ=255) cccTGGATCGGTTACTGGTGGAAACTGACTCACCTTACCTTGCGCCGGTACCGCTTCGAGGAAAAGAGAATCAACCTGCGATGGTTCGTGa > 2:551865/1‑91 (MQ=255) cccTGGATCGGTTACTGGTGGAAACTGACTCACCTTACCTTGCGCCGGTACCGCTTCGAGGAAAAGAGAATCAACCTGCGATGGTTCGTGa > 1:354129/1‑91 (MQ=255) tGGATCGGTTACTGGTGGAAACTGACTCACCTTACCTTGCGCCGGTACCGCTTCGAGGAAAAGAGAATCAACCTGCGATGGTTCGTGACGt < 2:396893/91‑1 (MQ=255) tGGATCGGTTACTGGTGGAAACTGACTCACCTTACCTTGCGCCGGTACCGCTTCGAGGAAAAGAGAATCAACCTGCGATGGTTCGTGACGt < 1:307148/91‑1 (MQ=255) aTCGGTTACTGGTGGAAACTGACTCACCTTACCTTGCGCCGGTACCGCTTCGAGGAAAAGAGAATCAACCTGCGATGGTTCGTGACGTTGc < 1:454358/91‑1 (MQ=255) aTCGGTTACTGGTGGAAACTGACTCACCTTACCTTGCGCCGGTACCGCTTCGAGGAAAAGAGAATCAACCTGCGATGGTTCGTGACGTTGc < 1:269176/91‑1 (MQ=255) ggTTACTGGTGGAAACTGACTCACCTTACCTTGCGCCGGTACCGCTTCGAGGAAAAGAGAATCAACCTGCGATGGTTCGTGACGTTGCAGa > 1:595915/1‑91 (MQ=255) ggTTACTGGTGGAAACTGACTCACCTTACCTTGCGCCGGTACCGCTTCGAGGAAAAGAGAATCAACCTGCGATGGTTCGTGACGTTGCAGa > 2:151719/1‑91 (MQ=255) tggtggAAACTGACTCACCTTACCTTGCGCCGGTACCGCTTCGAGGAAAAGAGAATCAACCTGCGATGGTTCGTGACGTTGCAGAATACAt < 1:511038/91‑1 (MQ=255) tggAAACTGACTCACCTTACCTTGCGCCGGTACCGCTTCGAGGAAAAGAGAATCAACCTGCGATGGTTCGTGACGTTGCAGAATACATGGc < 1:366362/91‑1 (MQ=255) tggAAACTGACTCACCTTACCTTGCGCCGGTACCGCTTCGAGGAAAAGAGAATCAACCTGCGATGGTTCGTGACGTTGCAGAATACATGGc < 1:85075/91‑1 (MQ=255) tggAAACTGACTCACCTTACCTTGCGCCGGTACCGCTTCGAGGAAAAGAGAATCAACCTGCGATGGTTCGTGACGTTGCAGAATACATGGc > 1:329195/1‑91 (MQ=255) ggAAACTGACTCACCTTACCTTGCGCCGGTACCGCTTCGAGGAAAAGAGAATCAACCTGCGATGGTTCGTGACGTTGCAGAATACATGGCt > 2:253348/1‑91 (MQ=255) gacaCTGACTCACCTTACCTTGCGCCGGTACCGCTTCGAGGAAAAGAGAATCAACCTGCGATGGTTCGTGACGTTGCAGAATACATGGCtg < 2:338211/88‑1 (MQ=255) gAAACTGACTCACCTTACCTTGCGCCGGTACCGCTTCGAGGAAAAGAGAATCAACCTGCGATGGTTCGTGACGTTGCAGAATACATGGCtg > 2:817449/1‑91 (MQ=255) gAAACTGACTCACCTTACCTTGCGCCGGTACCGCTTCGAGGAAAAGAGAATCAACCTGCGATGGTTCGTGACGTTGCAGAATACATGGCtg < 1:225310/91‑1 (MQ=255) aaCTGACTCACCTTACCTTGCGCCGGTACCGCTTCGAGGAAAAGAGAATCAACCTGCGATGGTTCGTGACGTTGCAGAATACATGGCtgtg > 2:287305/1‑91 (MQ=255) aCTCACCTTACCTTGCGCCGGTACCGCTTCGAGGAAAAGAGAATCAACCTGCGATGGTTCGTGACGTTGCAGAATACATGGCTGTGTTGaa < 1:77784/91‑1 (MQ=255) accttaccttGCGCCGGTACCGCTTCGAGGAAAAGAGAATCAACCTGCGATGGTTCGTGACGTTGCAGAATACATGGCTGTGTTGAAAGgt > 1:773171/1‑91 (MQ=255) ttaccttGCGCCGGTACCGCTTCGAGGAAAAGAGAATCAACCTGCGATGGTTCGTGACGTTGCAGAATACATGGCTGTGTTGAAAGGTGtt > 2:89551/1‑91 (MQ=255) gcgcCGGTACCGCTTCGAGGAAAAGAGAATCAACCTGCGATGGTTCGTGACGTTGCAGAATACATGGCTGTGTTGAAAGGTGTTGCCGTTg > 1:476403/1‑91 (MQ=255) gcgcCGGTACCGCTTCGAGGAAAAGAGAATCAACCTGCGATGGTTCGTGACGTTGCAGAATACATGGCTGTGTTGAAAGGTGTTGCCGTTg > 1:37373/1‑91 (MQ=255) gcgcCGGTACCGCTTCGAGGAAAAGAGAATCAACCTGCGATGGTTCGTGACGTTGCAGAATACATGGCTGTGTTGAAAGGTGTTGCCGTTg > 1:608532/1‑91 (MQ=255) ggTACCGCTTCGAGGAAAAGAGAATCAACCTGCGATGGTTCGTGACGTTGCAg < 2:463637/53‑1 (MQ=255) ggTACCGCTTCGAGGAAAAGAGAATCAACCTGCGATGGTTCGTGACGTTGCAg > 1:463637/1‑53 (MQ=255) | CGGAGCAACTGCGCGATGCTGCGCGTTATGTCCCCCTGGATCGGTTACTGGTGGAAACTGACTCACCTTACCTTGCGCCGGTACCGCATCGAGGAAAAGAGAATCAACCTGCGATGGTTCGTGACGTTGCAGAATACATGGCTGTGTTGAAAGGTGTTGCCGTTG > NZ_CP009273/1152786‑1152950 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |