Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,161,212	T→C	intergenic (+71/‑329)	ycfP → / → ndh	alpha/beta hydrolase YcfP/NADH‑quinone dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,161,212	0	T	C	100.0%	130.6 / NA	36	intergenic (+71/‑329)	ycfP/ndh	alpha/beta hydrolase YcfP/NADH‑quinone dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/25);  total (11/25)

AAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCACTC  >  NZ_CP009273/1161128‑1161296                                                                                     |                                                                                     aaccccTGGGGAAATTGCCCTCGTCGCATCAGGTACCCTTGCCGGTCCCTGTTGCGCTCCGAATTCTGTGGGTCGAATAAGGCCCCCAcgc                                                                                <  2:590706/88‑1 (MQ=255)      cTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCAt                                                                           >  1:155546/1‑91 (MQ=255)                tGCCCTCGTCGCATCAGGTAACCTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCg                                                                 <  2:417945/91‑1 (MQ=255)                 gCCCTCGTCGCATCAGGTAACCTTGCCGGTCCCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGa                                                                <  1:825811/91‑1 (MQ=255)                        tcgCATCAGGTAACCTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCaa                                                         <  1:25659/91‑1 (MQ=255)                                  tAACCTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATgc                                               >  2:45960/1‑91 (MQ=255)                                  tAACCTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATgc                                               <  1:377228/91‑1 (MQ=255)                                  tAACCTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATgc                                               <  2:361750/91‑1 (MQ=255)                                  tAACCTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATgc                                               <  1:788375/91‑1 (MQ=255)                                    aCCTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATgcgc                                             <  1:1608/91‑1 (MQ=255)                                       ttGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGcttc                                          <  1:724178/91‑1 (MQ=255)                                         gCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGCCCAcgc                                                                                >  2:674686/1‑51 (MQ=255)                                         gCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGCCCAcgc                                                                                <  1:674686/51‑1 (MQ=255)                                         gCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGcttctt                                        >  2:765616/1‑91 (MQ=255)                                         gCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGcttctt                                        >  2:839286/1‑91 (MQ=255)                                                  tGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCt                               >  2:641049/1‑91 (MQ=255)                                                       cgcTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGa                          >  1:267612/1‑91 (MQ=255)                                                        gcTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGaa                         >  2:20858/1‑91 (MQ=255)                                                         cTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAAc                        <  2:105049/91‑1 (MQ=255)                                                         cTCCGAATTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAAc                        >  2:44426/1‑91 (MQ=255)                                                              aaTTCTGTGGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCt                   <  2:493832/91‑1 (MQ=255)                                                                    gtgGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATac             <  2:127543/91‑1 (MQ=255)                                                                     tgGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATaca            <  2:98488/91‑1 (MQ=255)                                                                     tgGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATaca            <  2:697860/91‑1 (MQ=255)                                                                     tgGGTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATaca            <  1:344551/91‑1 (MQ=255)                                                                        gTCGGATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACAccc         <  2:581429/91‑1 (MQ=255)                                                                           ggATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTc      <  2:360366/91‑1 (MQ=255)                                                                           ggATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTc      <  2:590248/91‑1 (MQ=255)                                                                           ggATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTc      <  1:855966/91‑1 (MQ=255)                                                                           ggATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTc      <  1:466131/91‑1 (MQ=255)                                                                            gATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACg                       >  2:253483/1‑73 (MQ=255)                                                                            gATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACg                       <  1:253483/73‑1 (MQ=255)                                                                            gATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTc      <  2:441890/90‑1 (MQ=255)                                                                            gATAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTc      >  1:441890/1‑90 (MQ=255)                                                                             aTAAGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCCCAc    <  1:812902/91‑1 (MQ=255)                                                                               aaGGCGCCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCActc  <  2:522654/91‑1 (MQ=255)                                                                                     |                                                                                     AAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCACTC  >  NZ_CP009273/1161128‑1161296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑