Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	168,561	A→G	T205A (ACG→GCG)	fhuB →	Fe(3+)‑hydroxamate ABC transporter permease FhuB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	168,561	0	A	G	100.0%	65.8 / NA	18	T205A (ACG→GCG)	fhuB	Fe(3+)‑hydroxamate ABC transporter permease FhuB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/11);  total (7/11)

TTCTGTGGAGCACTGGAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGG  >  NZ_CP009273/168475‑168630                                                                                       |                                                                      ttCTGTGGAGCACTGGAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGGCGtt                                                                   <  1:247186/91‑1 (MQ=255) ttCTGTGGAGCACTGGAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGGCGtt                                                                   <  2:91655/91‑1 (MQ=255)                  aCGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGGCGTTGCTGCTACTTCGTCCGt                                                  <  1:699052/91‑1 (MQ=255)                   cGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTCATGGCCGCAGCTGCTGGGCGGTGTGATGCTGGCGTTGCTGCTACTTCGTCCGtt                                                 <  2:39622/91‑1 (MQ=255)                    gCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGGCGTTGCTGCTACTTCGTCCGTTa                                                <  1:633151/91‑1 (MQ=255)                       gACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGGCGTTGCTGCTACTTCGTCCGTTAAcc                                             >  2:428357/1‑91 (MQ=255)                                 gACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGGCGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGc                                   >  2:671764/1‑91 (MQ=255)                                   cTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGGCGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCtt                                 <  1:212774/91‑1 (MQ=255)                                          ggcgTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGGCGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGatgatg                          >  1:601285/1‑91 (MQ=255)                                           gcgTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGGCGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGAtgg                         >  2:622119/1‑91 (MQ=255)                                                     tttATGGCCGCAGCTGCTGGGCGGTGTGATGCTGGCGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGgcgcgc               <  1:360274/91‑1 (MQ=255)                                                     tttATGGCCGCAGCTGCTGGGCGGTGTGATGCTGGCGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGgcgcgc               <  2:494921/91‑1 (MQ=255)                                                     tttATGGCCGCAGCTGCTGGGCGGTGTGATGCTGGCGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGgcgcgc               <  2:525065/91‑1 (MQ=255)                                                       tATGGCCGCAGCTGCTGGGCGGTGTGATGCTGGCGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCaa             >  2:171830/1‑91 (MQ=255)                                                         tGGCCGCAGCTGCTGGGCGGTGTGATGCTGGCGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAAtc           <  2:265789/91‑1 (MQ=255)                                                          ggCCGCAGCTGCTGGGCGGTGTGATGCTGGCGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAAtct          >  2:122591/1‑91 (MQ=255)                                                            ccGCAGCTGCTGGGCGGTGTGATGCTGGCGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCg        >  2:184515/1‑91 (MQ=255)                                                                  ctgctgGGCGGTGTGATGCTGGCGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTgg  <  2:15739/91‑1 (MQ=255)                                                                                       |                                                                      TTCTGTGGAGCACTGGAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGG  >  NZ_CP009273/168475‑168630

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑