Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,182,954	T→A	D248V (GAT→GTT)	roxA ←	[50S ribosomal protein L16]‑arginine 3‑hydroxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,182,954	0	T	A	100.0%	123.4 / NA	33	D248V (GAT→GTT)	roxA	[50S ribosomal protein L16]‑arginine 3‑hydroxylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (17/16);  total (17/16)

TGACGTGACTGGGATATAAACTCGCCAAACCATTGCTTAAAGTGTTCCGGCTGGTTGATCAATTCGAGCATCATCTCACGCAGTTTATCCATCTCTTGCGGCAGAACATCCGCAGGATGAGCGCGAGGTGGAACATCCGGATCGCTGTAGTAGTTGCCGCCCAGTTCACGTTGC  >  NZ_CP009273/1182867‑1183040                                                                                        |                                                                                       tGACGTGACTGGGATATAAACTCGCCAAACCATTGCTTAAAGTGTTCCGGCTGGTTGATCAATTCGAGCATCATCTCACGCAGTTTAACCa                                                                                     >  2:592791/1‑91 (MQ=255) tGACGTGACTGGGATATAAACTCGCCAAACCATTGCTTAAAGTGTTCCGGCTGGTTGATCAATTCGAGCATCATCTCACGCAGTTTAACCa                                                                                     >  2:368315/1‑91 (MQ=255) tGACGTGACTGGGATATAAACTCGCCAAACCATTGCTTAAAGTGCTCCGGCTGGTTGATCAATTCGAGCATCATCTCACGCAGTTTAACCa                                                                                     <  1:196961/91‑1 (MQ=255)      tGACTGGGATATAAACTCGCCAAACCATTGCTTAAAGTGTTCCGGCTGGTTGATCAATTCGAGCATCATCTCACGCAGTTTAACCATctct                                                                                >  1:257929/1‑91 (MQ=255)      tGACTGGGATATAAACTCGCCAAACCATTGCTTAAAGTGTTCCGGCTGGTTGATCAATTCGAGCATCATCTCACGCAGTTTAACCATctct                                                                                >  2:158970/1‑91 (MQ=255)       gACTGGGATATAAACTCGCCAAACCATTGCTTAAAGTGTTCCGGCTGGTTGATCAATTCGAGCATCATCTCACGCAGTTTAACCATCTCtt                                                                               >  2:116303/1‑91 (MQ=255)             gATATAAACTCGCCAAACCATTGCTTAAAGTGTTCCGGCTGGTTGATCAATTCGAGCATCATCTCACGCAGTTTAACCATCTCTTGCGGCa                                                                         <  2:463381/91‑1 (MQ=255)                   aaCTCGCCAAACCATTGCTTAAAGTGTTCCGGCTGGTTGATCAATTCGAGCATCATCTCACGCAGTTTAACCATCTCTTGCGGCAGAACAt                                                                   <  1:676328/91‑1 (MQ=255)                      tCGCCAAACCATTGCTTAAAGTGTTCCGGCTGGTTGATCAATTCGAGCATCATCTCACGCAGTTTAACCATCTCTTGCGGCAGAACATCCg                                                                >  1:269131/1‑91 (MQ=255)                            aaCCATTGCTTAAAGTGTTCCGGCTGGTTGATCAATTCGAGCATCATCTCACGCAGTTTAACCATCTCTTGCGGCAGAACATCCGCAGGAt                                                          >  1:729922/1‑91 (MQ=255)                                 ttGCTTAAAGTGTTCCGGCTGGTTGATCAATTCGAGCATCATCTCACGCAGTTTAACCATCTCTTGCGGCAGAACATCCGCAGGATGAgcg                                                     >  2:357807/1‑91 (MQ=255)                                  tGCTTAAAGTGTTCCGGCTGGTTGATCAATTCGAGCATCATCTCACGCAGTTTAACCATCTCTTGCGGCAGAACATCCGCAGGATGAgcgc                                                    <  2:273899/91‑1 (MQ=255)                                        aaGTGTTCCGGCTGGTTGATCAATTCGAGCATCATCTCACGCAGTTTAACCATCTCTTGCGGCAGAACATCCGCAGGATGAGCGCGAGGTg                                              >  1:369565/1‑91 (MQ=255)                                          gtgtTCCGGCTGGTTGATCAATTCGAGCATCATCTCACGCAGTTTAACCATCTCTTGCGGCAGAACATCCGCAGGATGAGCGCGAGGTGGa                                            <  1:258244/91‑1 (MQ=255)                                          gtgtTCCGGCTGGTTGATCAATTCGAGCATCATCTCACGCAGTTTAACCATCTCTTGCGGCAGAACATCCGCAGGATGAGCGCGAGGTGGa                                            <  2:523552/91‑1 (MQ=255)                                          gtgtTCCGGCTGGTTGATCAATTCGAGCATCATCTCACGCAGTTTAACCATCTCTTGCGGCAGAACATCCGCAGGATGAGCGCGAGGTGGa                                            <  2:269131/91‑1 (MQ=255)                                                cGGCTGGTTGATCAATTCGAGCATCATCTCACGCAGTTTAACCATCTCTTGCGGCAGAACATCCGCAGGATGAGCGCGAGGTGGAACATcc                                      <  2:802793/91‑1 (MQ=255)                                                 ggCTGGTTGATCAATTCGAGCATCATCTCACGCAGTTTAACCATCTCTTGCGGCAGAACATCCGCAGGATGAGCGCGAGGTGGAACATCCg                                     >  1:690870/1‑91 (MQ=255)                                                  gCTGGTTGATCAATTCGAGCATCATCTCACGCAGTTTAACCATCTCTTGCGGCAGAACATCCGCAGGATGAGCGCGAGGTGGAACATCCgg                                    >  2:295711/1‑91 (MQ=255)                                                     ggTTGATCAATTCGAGCATCATCTCACGCAGTTTAACCATCTCTTGCGGCAGAACATCCGCAGGATGAGCGCGAGGTGGAACATCCGGATc                                 <  2:503865/91‑1 (MQ=255)                                                     ggTTGATCAATTCGAGCATCATCTCACGCAGTTTAACCATCTCTTGCGGCAGAACATCCGCAGGATGAGCGCGAGGTGGAACATCCGGATc                                 <  2:67551/91‑1 (MQ=255)                                                           tCAATTCGAGCATCATCTCACGCAGTTTAACCATCTCTTGCGGCAGAACATCCGCAGGATGAGCGCGAGGTGGAACATCCGGATCGCTgta                           >  1:55797/1‑91 (MQ=255)                                                              aTTCGAGCATCATCTCACGCAGTTTAACCATCTCTTGCGGCAGAACATCCGCAGGATGAGCGCGAGGTGGAACATCCGGATCGCTgtagta                        <  1:467446/91‑1 (MQ=255)                                                               ttCGAGCATCATCTCACGCAGTTTAACCATCTCTTGCGGCAGAACATCCGCAGGATGAGCGCGAGGTGGAACATCCGGATCGCTGtagtag                       >  2:475135/1‑91 (MQ=255)                                                                   aGCATCATCTCACGCAGTTTAACCATCTCTTGCGGCAGAACATCCGCAGGATGAGCGCGAGGTGGAACATCCGGATCGCTGTAGTAGTTgc                   <  1:295711/91‑1 (MQ=255)                                                                   aGCATCATCTCACGCAGTTTAACCATCTCTTGCGGCAGAACATCCGCAGGATGAGCGCGAGGTGGAACATCCGGATCGCTGTAGTAGTTgc                   >  1:710806/1‑91 (MQ=255)                                                                   aGCATCATCTCACGCAGTTTAACCATCTCTTGCGGCAGAACATCCGCAGGATGAGCGCGAGGTGGAACATCCGGATCGCTGTAGTAGTTgc                   <  1:457934/91‑1 (MQ=255)                                                                        catcTCACGCAGTTTAACCATCTCTTGCGGCAGAACATCCGCAGGATGAGCGCGAGGTGGAACATCCGGATCGCTGTAGTAGTTGCCGccc              >  1:606493/1‑91 (MQ=255)                                                                         atcTCACGCAGTTTAACCATCTCTTGCGGCAGAACATCCGCAGGATGAGCGCGAGGTGGAACATCCGGATCGCTGTAGTAGTTGCCGCCCa             >  1:49088/1‑91 (MQ=255)                                                                               cGCAGTTTAACCATCTCTTGCGGCAGAACATCCGCAGGATGAGCGCGAGGTGGAACATCCGGATCGCTGTAGTAGTTGCCGCCCAGTTCAc       <  1:97249/91‑1 (MQ=255)                                                                                   gTTTAACCATCTCTTGCGGCAGAACATCCGCAGGATGAGCGCGAGGTGGAACATCCGGATCGCTGTAGTAGTTGCCGCCCAGTTCACGTTg   >  2:139810/1‑91 (MQ=255)                                                                                    tttAACCATCTCTTGCGGCAGAACATCCGCAGGATGAGCGCGAGGTGGAACATCCGGATCGCTGTAGTAGTTGCCGCCCAGTTCACGTTgc  <  2:391956/91‑1 (MQ=255)                                                                                    tttAACCATCTCTTGCGGCAGAACATCCGCAGGATGAGCGCGAGGTGGAACATCCGGATCGCTGTAGTAGTTGCCGCCCAGTTCACGTTgc  <  2:462907/91‑1 (MQ=255)                                                                                        |                                                                                       TGACGTGACTGGGATATAAACTCGCCAAACCATTGCTTAAAGTGTTCCGGCTGGTTGATCAATTCGAGCATCATCTCACGCAGTTTATCCATCTCTTGCGGCAGAACATCCGCAGGATGAGCGCGAGGTGGAACATCCGGATCGCTGTAGTAGTTGCCGCCCAGTTCACGTTGC  >  NZ_CP009273/1182867‑1183040

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑