Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,220,495	T→A	D108V (GAT→GTT)	minD ←	septum site‑determining protein MinD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,220,495	0	T	A	100.0%	91.6 / NA	24	D108V (GAT→GTT)	minD	septum site‑determining protein MinD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (11/13);  total (11/13)

GCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTT  >  NZ_CP009273/1220411‑1220575                                                                                     |                                                                                 gCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCaa                                                                            >  2:228950/1‑91 (MQ=255)      aTAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACt                                                                       >  2:647427/1‑91 (MQ=255)         gagTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTg                                                                    >  2:387919/1‑91 (MQ=255)           gTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGc                                                                  >  1:217832/1‑91 (MQ=255)           gTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGc                                                                  >  1:858075/1‑91 (MQ=255)            tGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCg                                                                 <  1:286245/91‑1 (MQ=255)             gCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCGa                                                                >  1:153480/1‑91 (MQ=255)               cATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCGAcc                                                              <  1:403703/91‑1 (MQ=255)                 ttAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCGAcccc                                                            <  1:847712/91‑1 (MQ=255)                     cGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCa                                                        <  1:202723/91‑1 (MQ=255)                      gCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCAc                                                       <  1:284353/91‑1 (MQ=255)                        aCCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCACgg                                                     >  2:319807/1‑91 (MQ=255)                          cGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCACGGGt                                                   <  2:871070/91‑1 (MQ=255)                           ggTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCACGGGTg                                                  >  1:299812/1‑91 (MQ=255)                              ttCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCACGGGTGAgg                                               <  1:132243/91‑1 (MQ=255)                                cAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGc                                             <  1:597311/91‑1 (MQ=255)                                cAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCACAGGTGAGGGc                                             <  2:473672/91‑1 (MQ=255)                                  aTCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCAt                                           <  2:455071/91‑1 (MQ=255)                                           ggggAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATcgc                                  <  1:425392/91‑1 (MQ=255)                                                  cacaAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTg                           >  1:210908/1‑91 (MQ=255)                                                           tAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCgg                  <  1:319807/91‑1 (MQ=255)                                                                        cATCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAgaga     >  1:857101/1‑91 (MQ=255)                                                                         aTCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGAt    >  1:96420/1‑91 (MQ=255)                                                                           cGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGAttt  <  2:210908/91‑1 (MQ=255)                                                                                     |                                                                                 GCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTT  >  NZ_CP009273/1220411‑1220575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑