Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,223,250	T→C	V27V (GTT→GTC)	BW25113_RS06140 →	YcgL domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,223,250	0	T	C	100.0%	68.5 / NA	19	V27V (GTT→GTC)	BW25113_RS06140	YcgL domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/12);  total (7/12)

GTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCA  >  NZ_CP009273/1223177‑1223329                                                                          |                                                                                gtgtgATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTCCCTGAGGAACTGATGaa                                                                <  2:561440/91‑1 (MQ=255)  tgtgATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTCCCTGAGGAACTGATGaaa                                                               <  2:625328/91‑1 (MQ=255)  tgcgATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGAAGATTTTTCGCGTGTCCCTGAGGAACTGATGaaa                                                               >  2:378575/4‑91 (MQ=255)            cGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTCCCTGAGGAACTGATGAAAGGTTTTGGTc                                                     <  1:496928/91‑1 (MQ=255)            cGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTCCCTGAGGAACTGATGAAAGGTTTTGGTc                                                     <  1:681195/91‑1 (MQ=255)            cGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTCCCTGAGGAACTGATGAAAGGTTTTGGTc                                                     <  2:395238/91‑1 (MQ=255)                     aaGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTCCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGt                                            <  1:484602/91‑1 (MQ=255)                           gACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTCCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCga                                      >  2:632408/1‑91 (MQ=255)                                          tatGTCGAAAAAAAAGACGATTTTTCGCGTGTCCCTGAGGAACTGATGAAAGGTTTTGGTc                                                     <  1:134396/61‑1 (MQ=255)                                          tatGTCGAAAAAAAAGACGATTTTTCGCGTGTCCCTGAGGAACTGATGAAAGGTTTTGGTc                                                     >  2:134396/1‑61 (MQ=255)                                            tGTCGAAAAAAAAGACGATTTTTCGCGTGTCCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGAt                     >  1:537515/1‑91 (MQ=255)                                            tGTCGAAAAAAAAGACGATTTTTCGCGTGTCCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGAt                     <  2:144060/91‑1 (MQ=255)                                               cGAAAAAAAAGACGATTTTTCGCGTGTCCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATggg                  >  2:839273/1‑91 (MQ=255)                                               cGAAAAAAAAGACGATTTTTCGCGTGTCCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATggg                  >  2:778238/1‑91 (MQ=255)                                               cGAAAAAAAAGACGATTTTTCGCGTGTCCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATggg                  >  2:454191/1‑91 (MQ=255)                                               cGAAAAAAAAGACGATTTTTCGCGTGTCCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATggg                  >  2:182891/1‑91 (MQ=255)                                                    aaaaaGACGATTTTTCGCGTGTCCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGgaa             <  2:282478/91‑1 (MQ=255)                                                         gACGATTTTTCGCGTGTCCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAAc        <  2:367229/91‑1 (MQ=255)                                                         gACGATTTTTCGCGTGTCCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAAc        <  2:653043/91‑1 (MQ=255)                                                               ttttCGCGTGTCCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCa  <  1:572786/91‑1 (MQ=255)                                                                          |                                                                                GTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCA  >  NZ_CP009273/1223177‑1223329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑