| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,235,908 | T→G | A540A (GCA→GCC) | cvrA ← | potassium/proton antiporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,235,908 | 0 | T | G | 95.0% | 61.8 / ‑6.4 | 20 | A540A (GCA→GCC) | cvrA | potassium/proton antiporter |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base G (11/8); minor base C (1/0); total (12/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGG > NZ_CP009273/1235824‑1235983 | gCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGGGCcgcg > 2:134315/1‑91 (MQ=255) cTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGGGCCGCGCCTAACAActgctg > 2:337353/1‑91 (MQ=255) ttctttctCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGACAATTTCa > 1:240308/1‑91 (MQ=255) ctttctCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGACAATTTCAcc > 2:191286/1‑91 (MQ=255) ttctCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGACAATTTCACCCa > 2:342213/1‑91 (MQ=255) tcGGCCACCGTCCAGATCATCCCGGCAACCTCTACCTGGTCACCGACAACCGGCGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGcc > 1:647371/1‑90 (MQ=255) cACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTtgc < 2:834854/91‑1 (MQ=255) aGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATc < 1:402847/91‑1 (MQ=255) tcatcCCGGCAAACTCTACCTGGTCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACg < 1:512142/91‑1 (MQ=255) tcCCGGCAAACTCTACCTGGTCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGata > 1:600010/1‑91 (MQ=255) tcCCGGCAAACTCTACCTGGTCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGata > 2:685332/1‑91 (MQ=255) tcCCGGCAAACTCTACCTGGTCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGata > 2:568048/1‑91 (MQ=255) ccGGCAAACTCTACCTGGTCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATAtt > 1:640/1‑91 (MQ=255) gCAAACTCTACCTGGTCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATtctc < 2:712428/91‑1 (MQ=255) aCCTGGTCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCGGTCtt > 2:866898/1‑91 (MQ=255) ccTGGTCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCCGCTTATCACGATATTCTCGCCCGTCTTc < 2:607575/91‑1 (MQ=255) ggTCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAg < 2:556325/91‑1 (MQ=255) ggTCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAg < 1:136184/91‑1 (MQ=255) ggTCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAg < 1:134315/91‑1 (MQ=255) gTCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAgg > 1:773175/1‑91 (MQ=255) | GCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGG > NZ_CP009273/1235824‑1235983 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |