Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,260,284	A→G	D372G (GAC→GGC)	hemA →	glutamyl‑tRNA reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,260,284	0	A	G	100.0%	22.6 / NA	8	D372G (GAC→GGC)	hemA	glutamyl‑tRNA reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (4/4);  total (4/4)

CATTCGCGAGTATCGCAGCCAGGCAGAGCAAGTTCGCGATG‑AGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGC  >  NZ_CP009273/1260201‑1260368                                                                                     |                                                                                     cATCCGCGAGTATCGCAGCCAGGCAGAGCAAGTTCGCGATG‑AGTAACCCGCCAAAGCGTTAGCGGCCCTTGAGCGGGGCGGCGGCGCGaaa                                                                               <  2:154441/91‑1 (MQ=255)      gcgAGTATCGCAGCCAGGCAGAGCAAGTTCGCGATG‑AGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGGCGCGCAAGCCAt                                                                          <  1:625238/91‑1 (MQ=255)             tCGCAGCCAGGCAGAGCAAGTTCGCGATG‑AGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGGCGCGCAAGCCATTATGCAg                                                                   >  1:206043/1‑91 (MQ=255)             tCGCAGCCAGGCAGAGCAAGTTCGCGATG‑AGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGGCGCGCAAGCCATTATGCAg                                                                   >  1:352998/1‑91 (MQ=255)             ccGCAGCCAGGCAAAGCAGGTTCG‑GATGTAGTTAACCGCCAAGGGGTTGGCGCCGTTTGAGGGGGGGGCGGGCGCGAAAGCCATTATGCAg                                                                   <  2:181555/90‑1 (MQ=255)                       gCAGAGCAAGTTCGAGATG‑AGTAACCCGCAAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGGCGCGCAAGCCATTATGCAGGATCTGGCAt                                                         <  1:691563/91‑1 (MQ=255)                                  tCGCGATG‑AGTTAACCGCAAAAGCGTTAGCGGCCCTTGAGCGGGGCGGCGGCGCGAAAGCCATTATGCAGGATCTGGCATGGAAACTGACt                                              <  2:797362/91‑1 (MQ=255)                                    gagATG‑AGTTAACCGCAAAAGCTTTAGCGGCCCTTGAGCGGGGGGGCGGCGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTaa                                            <  1:774598/89‑1 (MQ=255)                                     ggatg‑atTAACCCGCCAAAGCGTTGGCGGCCCTTGAGCGGGGGGGCGGCGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAAc                                           <  1:5746/90‑1 (MQ=255)                                     ggATG‑AGTAACCCGCAAAGGCGTTAGCGCCCCTTGAGCGGGGCGGGGGCGCAAAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAAc                                           <  2:715134/90‑1 (MQ=255)                                                    ccAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGGCGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATgc                             >  2:273691/1‑91 (MQ=255)                                                             tAGCGGCCCTTGAGCAGGGCGGCGGCGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGaa                    >  2:551319/1‑91 (MQ=255)                                                                cGGCCCTTGAGCAGGGCGGCGGCGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATc                 <  2:352998/91‑1 (MQ=255)                                                                               gcggcggcgCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGgccgc  >  1:36482/1‑91 (MQ=255)                                                                                     |                                                                                     CATTCGCGAGTATCGCAGCCAGGCAGAGCAAGTTCGCGATG‑AGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGC  >  NZ_CP009273/1260201‑1260368

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑