Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,336,067	G→A	intergenic (+83/‑111)	lapB → / → pyrF	lipopolysaccharide assembly protein LapB/orotidine‑5'‑phosphate decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,336,067	0	G	A	100.0%	107.8 / NA	30	intergenic (+83/‑111)	lapB/pyrF	lipopolysaccharide assembly protein LapB/orotidine‑5'‑phosphate decarboxylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/11);  total (19/11)

TTTAAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATTGTGCTCCGGCAACGACGGCGCAGAAAAAGCCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCACGGGTAGAATGCTCGCCGTTTACCTGTTTCGCGCCACTTCCGG  >  NZ_CP009273/1335987‑1336152                                                                                 |                                                                                      tttAAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATTGTGCTCCGGCAACGACGGCGCAGAAAAAACCTGCCAggg                                                                             >  2:489574/1‑91 (MQ=255)                  tttAGTTACAACATACTAATTATTAATGTTCCATTGTGCTCCGGCAACGACGGCGCAGAAAAAACCTGCCAGGGGAGAAATCGCAACTGtt                                                            <  1:17648/91‑1 (MQ=255)                               tACTAATTATTAATGTTCCATTGTGCTCCGGCAACGACGGCGCAGAAAAAACCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTc                                               >  2:726051/1‑91 (MQ=255)                               tACTAATTATTAATGTTCCATTGTGCTCCGGCAACGACGGCGCAGAAAAAACCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTc                                               >  2:617269/1‑91 (MQ=255)                               tACTAATTATTAATGTTCCATTGTGCTCCGGCAACGACGGCGCAGAAAAAACCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTc                                               >  1:694213/1‑91 (MQ=255)                                aCTAATTATTAATGTTCCATTGTGCTCCGGCAACGACGGCGCAGAAAAAACCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTcc                                              <  2:712995/91‑1 (MQ=255)                                  tAATTATTAATGTTCCATTGTGCTCCGGCAACGACGGCGCAGAAAAAACCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCAc                                            <  2:215883/91‑1 (MQ=255)                                   aaTTATTAATGTTCCATTGTGCTCCGGCAACGACGGCGCAGAAAAAACCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCACg                                           >  2:860168/1‑91 (MQ=255)                                        ttaATGTTCCATTGTGCTCCGGCAACGACGGCGCAGAAAAAACCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCACGGGTAg                                      >  1:191722/1‑91 (MQ=255)                                         taATGTTCCATTGTGCTCCGGCAACGACGGCGCAGAAAAAACCTGCCAGGGGAGaa                                                                        >  1:232541/1‑56 (MQ=255)                                         taATGTTCCATTGTGCTCCGGCAACGACGGCGCAGAAAAAACCTGCCAGGGGAGaa                                                                        <  2:232541/56‑1 (MQ=255)                                             gTTCCATTGTGCTCCGGCAACGACGGCGCAGAAAAAACCTGCCAGGGGAGCAATCGCAACTGTTAATTTTTTATTTCCACGGGTAGAGTGc                                 >  2:733767/1‑91 (MQ=255)                                               tCCATTGTGCTCCGGCAACGACGGCGCAGAAAAAACCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCACGGGTAGAATGCTc                               >  1:766536/1‑91 (MQ=255)                                               tCCATTGTGCTCCGGCAACGACGGCGCAGAAAAAACCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCACGGGTAGAATGCTc                               <  1:718005/91‑1 (MQ=255)                                               tCCATTGTGCTCCGGCAACGACGGCGCAGAAAAAACCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCACGGGTAGAATGCTc                               >  2:670174/1‑91 (MQ=255)                                               tCCATTGTGCTCCGGCAACGACGGCGCAGAAAAAACCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCACGGGTAGAATGCTc                               >  2:642373/1‑91 (MQ=255)                                               tCCATTGTGCTCCGGCAACGACGGCGCAGAAAAAACCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCACGGGTAGAATGCTc                               >  2:474228/1‑91 (MQ=255)                                                 cATTGTGCTCCGGCAACGACGGCGCAGAAAAAACCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCACGGGTAGAATGCTCGc                             >  2:322849/1‑91 (MQ=255)                                                    tgtgCTCCGGCAACGACGGCGCAGAAAAAACCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCACGGGTAGAATGCTCGCCGt                          <  2:118360/91‑1 (MQ=255)                                                     gtgCTCCGGCAACGACGGCGCAGAAAAAACCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCACGGGTAGAATGCTCGCCGtt                         >  2:376677/1‑91 (MQ=255)                                                       gCTCCGGCAACGACGGCGCAGAAAAAACCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCAc                                            <  1:21307/70‑1 (MQ=255)                                                       gCTCCGGCAACGACGGCGCAGAAAAAACCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCAc                                            >  2:21307/1‑70 (MQ=255)                                                       gCTCCGGCAACGACGGCGCAGAAAAAACCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCAc                                            <  2:663662/70‑1 (MQ=255)                                                       gCTCCGGCAACGACGGCGCAGAAAAAACCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCAc                                            >  1:663662/1‑70 (MQ=255)                                                       gCTCCGGCAACGACGGCGCAGAAAAAACCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCACGGGTAGAATGCTCGCCGTTTa                       >  1:709613/1‑91 (MQ=255)                                                         tCCGGCAACGACGGCGCAGAAAAAACCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCACGGGTAGAATGCTCGCCGTTTAcc                     <  1:693687/91‑1 (MQ=255)                                                          ccGGCAACGACGGCGCAGAAAAAACCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCACGGGTAGAATGCTCGCCGTTTACCt                    >  2:295883/1‑91 (MQ=255)                                                               aaCGACGGCGCAGAAAAAACCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCACGGGTAGAATGCTCGCCGTTTACCTGTTTc               <  1:489574/91‑1 (MQ=255)                                                               aaCGACGGCGCAGAAAAAACCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCACGGGTAGAATGCTCGCCGTTTACCTGTTTc               <  1:860168/91‑1 (MQ=255)                                                                  gacgGGGCAGAAAAAACGTGCCAGGGGAGAAATCGCAACGGTTAATTTTTTATTTCCGCGGGTAGAATGCGTGCCGTTTCCCTGTGTcgcg            <  2:156152/91‑1 (MQ=255)                                                                      gcgcAGAAAAAACCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCACGGGTAGAATGCTCGCCGTTTACCTGTTTCGCGCCAc        >  2:429259/1‑91 (MQ=255)                                                                            aaaaaaCCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCACGGGTAGAATGCTCGCCGTTTACCTGTTTCGCGCCACTTCCgg  <  2:34603/91‑1 (MQ=255)                                                                            aaaaaaCCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCACGGGTAGAATGCTCGCCGTTTACCTGTTTCGCGCCACTTCCgg  <  1:670174/91‑1 (MQ=255)                                                                                 |                                                                                      TTTAAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATTGTGCTCCGGCAACGACGGCGCAGAAAAAGCCTGCCAGGGGAGAAATCGCAACTGTTAATTTTTTATTTCCACGGGTAGAATGCTCGCCGTTTACCTGTTTCGCGCCACTTCCGG  >  NZ_CP009273/1335987‑1336152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑