Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,449,980	A→G	D187G (GAT→GGT)	paaC →	phenylacetate‑CoA oxygenase subunit PaaC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,449,980	0	A	G	100.0%	140.4 / NA	38	D187G (GAT→GGT)	paaC	phenylacetate‑CoA oxygenase subunit PaaC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/13);  total (25/13)

CATCAACAAGTTGTGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGT  >  NZ_CP009273/1449894‑1450066                                                                                       |                                                                                       cATCAACAAGTTGTGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGGTCCa                                                                                    >  1:174188/1‑91 (MQ=255)     aacaaGTTGTGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGGTCCACGCa                                                                                <  1:595786/91‑1 (MQ=255)             gtgGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGGTCCACGCACTTTAcgc                                                                        >  2:528350/1‑91 (MQ=255)                          gccgAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAg                                                           >  1:535472/1‑91 (MQ=255)                          gccgAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAg                                                           >  1:403583/1‑91 (MQ=255)                                  gTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGtt                                                   >  2:869259/1‑91 (MQ=255)                                         gCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCg                                            <  2:450538/91‑1 (MQ=255)                                           cgacgaGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCggg                                          <  2:455427/91‑1 (MQ=255)                                            gacgaGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGGTCCACGCACTTTACGCGCAGCGTGGAAAGCCGAAGTTTTTGCCGGGa                                         >  2:135656/1‑91 (MQ=255)                                                 gaTTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAAc                                    <  1:190047/91‑1 (MQ=255)                                                          tGCACTGAGTGAAGAGGGTATTGCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCcaca                           >  1:759903/1‑91 (MQ=255)                                                           gCACTGAGTGAAGAGGGTATTGCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACAt                          >  2:869221/1‑91 (MQ=255)                                                                gAGTGAAGAGGGTATTGCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAAc                     >  2:482523/1‑91 (MQ=255)                                                                gAGTGAAGAGGGTATTGCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAAc                     >  1:702208/1‑91 (MQ=255)                                                                gAGTGAAGAGGGTATTGCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAAc                     >  1:431632/1‑91 (MQ=255)                                                                gAGTGAAGAGGGTATTGCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAAc                     <  1:330036/91‑1 (MQ=255)                                                                gAGTGAAGAGGGTATTGCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAAc                     >  1:262631/1‑91 (MQ=255)                                                                gAGTGAAGAGGGTATTGCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAAc                     >  1:229774/1‑91 (MQ=255)                                                                gAGTGAAGAGGGTATTGCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAAc                     >  2:88826/1‑91 (MQ=255)                                                                  gTGAAGAGGGTATTGCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGt                   <  1:12298/91‑1 (MQ=255)                                                                    gAAGAGGGTATTGCGGTTGGTCCCCGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTAc                 <  1:385332/91‑1 (MQ=255)                                                                          ggTATTGCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCg                                            <  2:113782/58‑1 (MQ=255)                                                                          ggTATTGCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCg                                            >  1:113782/1‑58 (MQ=255)                                                                          ggTATTGCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAag           >  2:360688/1‑91 (MQ=255)                                                                          ggTATTGCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAag           >  1:432819/1‑91 (MQ=255)                                                                          ggTATTGCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAag           >  1:327718/1‑91 (MQ=255)                                                                          ggTATTGCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAag           >  1:133555/1‑91 (MQ=255)                                                                            tATTGCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAagag         >  1:58878/1‑91 (MQ=255)                                                                            tATTGCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAagag         >  2:525777/1‑91 (MQ=255)                                                                             aTTGCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGc        <  2:9543/91‑1 (MQ=255)                                                                              ttGCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGt                   >  2:639702/1‑79 (MQ=255)                                                                              ttGCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGt                   <  1:639702/79‑1 (MQ=255)                                                                              ttGCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCa       <  2:432819/91‑1 (MQ=255)                                                                               tGCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAg      >  1:323652/1‑91 (MQ=255)                                                                                gCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAgg     <  2:328872/91‑1 (MQ=255)                                                                                gCGGTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAgg     <  2:535472/91‑1 (MQ=255)                                                                                   gTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGt  >  2:400340/1‑91 (MQ=255)                                                                                   gTTGGTCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGt  >  1:397122/1‑91 (MQ=255)                                                                                       |                                                                                       CATCAACAAGTTGTGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGT  >  NZ_CP009273/1449894‑1450066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑