Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,455,278	T→G	R43R (CGT→CGG)	paaJ →	bifunctional 3‑oxoadipyl‑CoA/3‑oxo‑5,6‑dehydrosuberyl‑CoA thiolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,455,278	0	T	G	100.0%	103.9 / NA	29	R43R (CGT→CGG)	paaJ	bifunctional 3‑oxoadipyl‑CoA/3‑oxo‑5,6‑dehydrosuberyl‑CoA thiolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (13/16);  total (13/16)

TTGGTCGCTACGGCGGGGCATTATCAAGTGTTCGGGCTGATGATCTGGCTGCTATCCCTTTGCGGGAACTGCTGGTGCGAAACCCGCGTCTCGATGCGGAGTGTATCGATGATGTGATCCTCGGCTGTGCTAATCAGGCGGGAGAAGATAACCGTAACGTAGCCCGGAT  >  NZ_CP009273/1455190‑1455358                                                                                         |                                                                                 ttGGTCGCTACGGCGGGGCATTATCAAGTGTTCGGGCTGATGATCTGGCTGCTATCCCTTTGCGGGAACTGCTGGTGCGAAACCCGCGgct                                                                                <  1:521225/91‑1 (MQ=255)       gCTACGGCGGGGCATTATCAAGTGTTCGGGCTGATGATCTGGCTGCTATCCCTTTGCGGGAACTGCTGGTGCGAAACCCGCGGCTCGATGc                                                                          <  1:24083/91‑1 (MQ=255)         tACGGCGGGGCATTATCAAGTGTTCGGGCTGATGATCTGGCTGCTATCCCTTTGCGGGAACTGCTGGTGCGAAACCCGCGGCTCGATGCgg                                                                        <  2:811390/91‑1 (MQ=255)         tACGGCGGGGCATTATCAAGTGTTCGGGCTGATGATCTGGCTGCTATCCCTTTGCGGGAACTGCTGGTGCGAAACCCGCGGCTCGATGCgg                                                                        <  2:665676/91‑1 (MQ=255)         tACGGCGGGGCATTATCAAGTGTTCGGGCTGATGATCTGGCTGCTATCCCTTTGCGGGAACTGCTGGTGCGAAACCCGCGGCTCGATGCgg                                                                        <  2:428976/91‑1 (MQ=255)           cggcggGGCATTATCAAGTGTTCGGGCTGATGATCTGGCTGCTATCCCTTTGCGGGAACTGCTGGTGCGAAACCCGCGGCTCGATGCGGAg                                                                      <  1:289223/91‑1 (MQ=255)            ggcggGGCATTATCAAGTGTTCGGGCTGATGATCTGGCTGCTATCCCTTTGCGGGAACTGCTGGTGCGAAACCCGCGGCTCGATGCGGAgt                                                                     <  2:33830/91‑1 (MQ=255)             gcggGGCATTATCAAGTGTTCGGGCTGATGATCTGGCTGCTATCCCTTTGCGGGAACTGCTGGTGCGAAACCCGCGGCTCGGTGCGGAgtg                                                                    >  2:819274/1‑91 (MQ=255)                gggCATTATCAAGTGTTCGGGCTGATGATCTGGCTGCTATCCCTTTGCGGGAACTGCTGGTGCGAAACCCGCGGCTCGATGCGGAGTGTAt                                                                 >  1:279132/1‑91 (MQ=255)                gggCATTATCAAGTGTTCGGGCTGATGATCTGGCTGCTATCCCTTTGCGGGAACTGCTGGTGCGAAACCCGCGGCTCGATGCGGAGCGTAt                                                                 <  1:828910/91‑1 (MQ=255)                 ggCATTATCAAGTGTTCGGGCTGATGATCTGGCTGCTATCCCTTTGCGGGAACTGCTGGTGCGAAACCCGCGGCTCGATGCGGAGTGTATc                                                                <  1:677967/91‑1 (MQ=255)                         cAAGTGTTCGGGCTGATGATCTGGCTGCTATCCCTTTGCGGGAACTGCTGGTGCGAAACCCGCGGCTCGATGCGGAGTGTATCGATGAtgt                                                        <  2:545253/91‑1 (MQ=255)                            gtgtTCGGGCTGATGATCTGGCTGCTATCCCTTTGCGGGAACTGCTGGTGCGAAACCCGCGGCTCGATGCGGAGTGTATCGATGATGTGAt                                                     >  1:650390/1‑91 (MQ=255)                              gtTCGGGCTGATGATCTGGCTGCTATCCCTTTGCGGGAACTGCTGGTGCGAAACCCGCGGCTCGATGCGGAGTGTATCGATGATGTGATcc                                                   >  1:519140/1‑91 (MQ=255)                              gtTCGGGCTGATGATCTGGCTGCTATCCCTTTGCGGGAACTGCTGGTGCGAAACCCGCGGCTCGATGCGGAGTGTATCGATGATGTGATcc                                                   >  1:105754/1‑91 (MQ=255)                              gtTCGGGCTGATGATCTGGCTGCTATCCCTTTGCGGGAACTGCTGGTGCGAAACCCGCGGCTCGATGCGGAGTGTATCGATGATGTGATcc                                                   >  1:797033/1‑91 (MQ=255)                              gtTCGGGCTGATGATCTGGCTGCTATCCCTTTGCGGGAACTGCTGGTGCGAAACCCGCGGCTCGATGCGGAGTGTATCGATGATGTGATcc                                                   >  1:815370/1‑91 (MQ=255)                                 cGGGCTGATGATCTGGCTGCTATCCCTTTGCGGGAACTGCTGGTGCGAAACCCGCGGCTCGATGCGGAGTGTATCGATGATGTGATCCTCg                                                >  2:767622/1‑91 (MQ=255)                                   ggCTGATGATCTGGCTGCTATCCCTTTGCGGGAACTGCTGGTGCGAAACCCGCGGCTCGATGCGGAGTGTATCGATGATGTGATCCTCGGc                                              <  2:358386/91‑1 (MQ=255)                                   ggCTGATGATCTGGCTGCTATCCCTTTGCGGGAACTGCTGGTGCGAAACCCGCGGCTCGATGCGGAGTGTATCGATGATGTGATCCTCGGc                                              <  2:650390/91‑1 (MQ=255)                                                    ctATCCCTTTGCGGGAACTGCTGGTGCGAAACCCGCGGCTCGATGCGGAGTGTATCGATGATGTGATCCTCGGCTGTGCTAATCAGGCggg                             <  1:456830/91‑1 (MQ=255)                                                    ctATCCCTTTGCGGGAACTGCTGGTGCGAAACCCGCGGCTCGATGCGGAGTGTATCGATGATGTGATCCTCGGCTGTGCTAATCAGGCggg                             <  2:815370/91‑1 (MQ=255)                                                    ctATCCCTTTGCGGGAACTGCTGGTGCGAAACCCGCGGCTCGATGCGGAGTGTATCGATGATGTGATCCTCGGCTGTGCTAATCAGGCggg                             <  2:23294/91‑1 (MQ=255)                                                      aTCCCTTTGCGGGAACTGCTGGTGCGAAACCCGCGGCTCGATGCGGAGTGTATCGATGATGTGATCCTCGGCTGTGCTAATCAGGCGGgag                           >  1:278691/1‑91 (MQ=255)                                                         ccTTTGCGGGAACTGCTGGTGCGAAACCCGCGGCTCGATGCGGAGTGTATCGATGATGTGATCCTCGGCTGTGCTAATCAGGCGGGagaag                        >  1:325078/1‑91 (MQ=255)                                                           tttGCGGGAACTGCTGGTGCGAAACCCGCGGCTCGATGCGGAGTGTATCGATGATGTGATCCTCGGCTGTGCTAATCAGGCGGGCGAGGAt                      >  2:817198/1‑91 (MQ=255)                                                                 ggAACTGCTGGTGCGAAACCCGCGGCTCGATGCGGAGTGTATCGATGATGTGATCCTCGGCTGTGCTAATCAGGCGGGAGAAGATAACCGt                <  2:507918/91‑1 (MQ=255)                                                                       gctgGTGCGAAACCCGCGGCTCGATGCGGAGTGTATCGATGATGTGATCCTCGGCTGTGCTAATCAGGCGGGAGAAGATAACCGTAACGTa          >  2:407656/1‑91 (MQ=255)                                                                              cGAAACCCGCGGCTCGATGCGGAGTGTATCGATGATGTGATCCTCGGCTGTGCTAATCAGGCGGGAGAAGATAACCGTAACGTAGCCCGGa   <  1:268866/91‑1 (MQ=255)                                                                               gAAACCCGCGGCTCGATGCGGAGTGTATCGATGATGTGATCCTCGGCTGTGCTAATCAGGCGGGAGAAGATAACCGTAACGTAGCCCGGAt  >  1:662293/1‑91 (MQ=255)                                                                                         |                                                                                 TTGGTCGCTACGGCGGGGCATTATCAAGTGTTCGGGCTGATGATCTGGCTGCTATCCCTTTGCGGGAACTGCTGGTGCGAAACCCGCGTCTCGATGCGGAGTGTATCGATGATGTGATCCTCGGCTGTGCTAATCAGGCGGGAGAAGATAACCGTAACGTAGCCCGGAT  >  NZ_CP009273/1455190‑1455358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑