Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,492,661	A→G	M517V (ATG→GTG)	opgD →	glucan biosynthesis protein OpgD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,492,661	0	A	G	100.0%	181.3 / NA	48	M517V (ATG→GTG)	opgD	glucan biosynthesis protein OpgD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/26);  total (22/26)

TATTGAACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTA  >  NZ_CP009273/1492582‑1492743                                                                                |                                                                                   tatTGAACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGt                                                                         <  2:342807/91‑1 (MQ=255) tatTGAACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGt                                                                         >  1:96687/1‑91 (MQ=255)     gAACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTc                                                                     >  2:384204/1‑91 (MQ=255)     gAACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTc                                                                     >  2:509963/1‑91 (MQ=255)      aaCCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCa                                                                    >  1:5720/1‑91 (MQ=255)        cccATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAgg                                                                  >  1:331372/1‑91 (MQ=255)         ccATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAggg                                                                 <  2:368370/91‑1 (MQ=255)         ccATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAggg                                                                 <  2:665213/91‑1 (MQ=255)         ccATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAggg                                                                 <  2:77097/91‑1 (MQ=255)          cATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAgggg                                                                >  1:235340/1‑91 (MQ=255)          cATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAgggg                                                                >  1:867498/1‑91 (MQ=255)           aTCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAggggg                                                               <  2:331372/91‑1 (MQ=255)            tCGATGGTTATCGTATTCAGTTTGACTGGGATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAgggggc                                                              >  2:293415/1‑90 (MQ=255)             cGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGAc                                                             <  2:235340/91‑1 (MQ=255)             cGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGAc                                                             <  2:867498/91‑1 (MQ=255)             cGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGAc                                                             <  2:512024/91‑1 (MQ=255)                 ggTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTa                                                         <  2:619021/91‑1 (MQ=255)                  gTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTAt                                                        <  2:861545/91‑1 (MQ=255)                    tATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCa                                                      >  1:121982/1‑91 (MQ=255)                    tATCGTATTCAGTTCGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCa                                                      >  1:746514/1‑91 (MQ=255)                     aTCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAg                                                     >  1:373876/1‑91 (MQ=255)                      tCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGt                                                    <  2:668657/91‑1 (MQ=255)                        gTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGa                                                  <  2:58852/91‑1 (MQ=255)                        gTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGa                                                  <  2:447170/91‑1 (MQ=255)                          aTTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGaaa                                                >  2:295862/1‑91 (MQ=255)                           ttCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAAc                                               <  1:219844/91‑1 (MQ=255)                           atCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAAc                                               <  2:569073/90‑1 (MQ=255)                            tCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTTGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACa                                              >  1:586153/1‑91 (MQ=255)                            tCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTg                                                   >  1:510021/1‑86 (MQ=255)                            tCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTg                                                   <  2:510021/86‑1 (MQ=255)                                    aCTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTa                                      >  2:550390/1‑91 (MQ=255)                                       ggTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCa                                   <  2:43477/91‑1 (MQ=255)                                                  tCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGc                        >  1:676752/1‑91 (MQ=255)                                                  tCGGACTCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGc                        >  2:10789/1‑91 (MQ=255)                                                        tCCACTGATCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAgcgc                  <  2:121982/91‑1 (MQ=255)                                                               aTCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATaa           <  2:96687/91‑1 (MQ=255)                                                               aTCCGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATaa           <  1:445338/91‑1 (MQ=255)                                                                 ccGGTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAAc         <  1:175517/91‑1 (MQ=255)                                                                   ggTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTa                                                         >  2:738209/1‑41 (MQ=255)                                                                   ggTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTa                                                         <  1:738209/41‑1 (MQ=255)                                                                   ggTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGt       >  1:792333/1‑91 (MQ=255)                                                                   ggTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGt       >  1:755534/1‑91 (MQ=255)                                                                   ggTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGt       >  1:735162/1‑91 (MQ=255)                                                                    gTCGATATGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTc      <  2:637314/91‑1 (MQ=255)                                                                        atatGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTa  <  2:539317/91‑1 (MQ=255)                                                                        atatGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTa  <  1:755211/91‑1 (MQ=255)                                                                        atatGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTa  <  2:676752/91‑1 (MQ=255)                                                                        atatGCGGGTGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTa  >  2:758541/1‑91 (MQ=255)                                                                                |                                                                                   TATTGAACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTA  >  NZ_CP009273/1492582‑1492743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑