Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,575,730	T→C	D351G (GAT→GGT)	ydeN ←	sulfatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,575,730	0	T	C	100.0%	121.2 / NA	32	D351G (GAT→GGT)	ydeN	sulfatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/16);  total (16/16)

TAAACATTGGGGTGTGAGTACCGCCAGGATAGGTCTGACTCTTATAGCCTTTTTGCGCCCCGTTCAGCGGCAGAGGACCATCGATAACCGCACCATTATCGGAGGTAAAGAGAATAATTGTATTGTCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTT  >  NZ_CP009273/1575650‑1575815                                                                                 |                                                                                      tAAACATTGGGGTGTGAGTACCGCCAGGATAGGTCTGACTCTTATAGCCTTTTTGCGCCCCGTTCAGCGGCAGAGGACCACCGATAACCGc                                                                             <  2:802870/91‑1 (MQ=255) tAAACATTGGGGTGTGAGTACCGCCAGGATAGGTCTGACTCTTATAGCCTTTTTGCGCCCCGTTCAGCGGCAGAGGACCACCGATAACCGc                                                                             <  1:390339/91‑1 (MQ=255)  aaaCATTGGGGTGTGAGTACCGCCAGGATAGGTCTGACTCTTATAGCCTTTTTGCGCCCCGTTCAGCGGCAGAGGACCACCGATAACCGCa                                                                            <  2:612567/91‑1 (MQ=255)   aaCATTGGGGTGTGAGTACCGCCAGGATAGGTCTGACTCTTATAGCCTTTTTGCGCCCCGTTCAGCGGCAGAGGACCACCGATAACCGCAc                                                                           <  2:232492/91‑1 (MQ=255)        tGGGGTGTGAGTACCGCCAGGATAGGTCTGACTCTTATAGCCTTTTTGCGCCCCGTTCAGCGGCAGAGGACCACCGATAACCGCACCATTa                                                                      >  1:474545/1‑91 (MQ=255)         ggggTGTGAGTACCGCCAGGATAGGTCTGACTCTTATAGCCTTTTTGCGCCCCGTTCAGCGGCAGAGGACCACCGATAACCGCACCATTAt                                                                     >  2:780111/1‑91 (MQ=255)         ggggTGTGAGTACCGCCAGGATAGGTCTGACTCTTATAGCCTTTTTGCGCCCCGTTCAGCGGCAGAGGACCACCGATAACCGCACCATTAt                                                                     >  2:745745/1‑91 (MQ=255)              gtgAGTACCGCCAGGATAGGTCTGACTCTTATAGCCTTTTTGCGCCCCGTTCAGCGGCAGAGGACCACCGATAACCGCACCATTATCGGAg                                                                >  2:822218/1‑91 (MQ=255)              gtgAGTACCGCCAGGATAGGTCTGACTCTTATAGCCTTTTTGCGCCCCGTTCAGCGGCAGAGGACCACCGATAACCGCACCATTATCGGAg                                                                >  2:564202/1‑91 (MQ=255)              gtgAGTACCGCCAGGATAGGTCTGACTCTTATAGCCTTTTTGCGCCCCGTTCAGCGGCAGAGGACCACCGATAACCGCACCATTATCGGAg                                                                >  2:359521/1‑91 (MQ=255)                  gTACCGCCAGGATAGGTCTGACTCTTATAGCCTTTTTGCGCCCCGTTCAGCGGCAGAGGACCACCGATAACCGCACCATTATCGGAGGTaa                                                            <  1:339460/91‑1 (MQ=255)                    aCCGCCAGGATAGGTCTGACTCTTATAGCCTTTTTGCGCCCCGTTCAGCGGCAGAGGACCACCGATAACCGCACCATTATCGGAGGTAAag                                                          <  1:138865/91‑1 (MQ=255)                    aCCGCCAGGATAGGTCTGACTCTTATAGCCTTTTTGCGCCCCGTTCAGCGGCAGAGGACCACCGATAACCGCACCATTATCGGAGGTAAag                                                          <  1:138832/91‑1 (MQ=255)                          aGGATAGGTCTGACTCTTATAGCCTTTTTGCGCCCCGTTCAGCGGCAGAGGACCACCGATAAGCGCACCATTAGCGGAGGTAAAGAGaata                                                    >  2:45766/1‑91 (MQ=255)                          aGGATAGGTCTGACTCTTATAGCCTTTTTGCGCCCCGTTCAGCGGCAGAGGACCACCGATAACCGCACCATTATCGGAGGTAAAGAGaata                                                    >  1:795023/1‑91 (MQ=255)                          aGGATAGGTCTGACTCTTATAGCCTTTTTGCGCCCCGTTCAGCGGCAGAGGACCACCGATAACCGCACCATTATCGGAGGTAAAGAGaata                                                    >  2:857689/1‑91 (MQ=255)                               aGGTCTGACTCTTATAGCCTTTTTGCGCCCCGTTCAGCGGCAGAGGACCACCGATAACCGCACCATTATCGGAGGTAAAGAGAATAattgt                                               <  1:780111/91‑1 (MQ=255)                               aGGTCTGACTCTTATAGCCTTTTTGCGCCCCGTTCAGCGGCAGAGGACCACCGATAACCGCACCATTATCGGAGGTAAAGAGAATAattgt                                               <  2:869747/91‑1 (MQ=255)                               aGGTCTGACTCTTATAGCCTTTTTGCGCCCCGTTCAGCGGCAGAGGACCACCGATAACCGCACCATTATCGGAGGTAAAGAGAATAattgt                                               <  1:471684/91‑1 (MQ=255)                               aGGTCTGACTCTTATAGCCTTTTTGCGCCCCGTTCAGCGGCAGAGGACCACCGATAACCGCACCATTATCGGAGGTAAAGAGAATAattgt                                               <  2:255233/91‑1 (MQ=255)                               aGGTCTGACTCTTATAGCCTTTTTGCGCCCCGTTCAGCGGCAGAGGACCACCGATAACCGCACCATTATCGGAGGTAAAGAGAATAattgt                                               >  2:254365/1‑91 (MQ=255)                               aGGTCTGACTCTTATAGCCTTTTTGCGCCCCGTTCAGCGGCAGAGGACCACCGATAACCGCACCATTATCGGAGGTAAAGAGAATAattgt                                               <  1:745745/91‑1 (MQ=255)                                 gTCTGACTCTTATAGCCTTTTTGCGCCCCGTTCAGCGGCAGAGGACCACCGATAACCGCACCATTATCGGAGGTAAAGAGAATAattgtat                                             <  1:650094/91‑1 (MQ=255)                                           tataGCCTTTTTGCGCCCCGTTCAGCGGCAGAGGACCACCGATAACCGCACCATTATCGGAGGTAAAGAGAATAattgt                                               >  2:271166/1‑79 (MQ=255)                                           tataGCCTTTTTGCGCCCCGTTCAGCGGCAGAGGACCACCGATAACCGCACCATTATCGGAGGTAAAGAGAATAattgt                                               <  1:271166/79‑1 (MQ=255)                                           tataGCCTTTTTGCGCCCCGTTCAGCGGCAGAGGACCACCGATAACCGCACCATTATCGGAGGTAAAGAGAATAATTGTATTGTCATACTg                                   >  1:656408/1‑91 (MQ=255)                                                           cccGTTCAGCGGCAGAGGACCACCGATAACCGCACCATTATCGGAGGTAAAGAGAATAATTGTATTGTCATACTGTCCGTTTTTCTTCAgt                   <  2:748340/91‑1 (MQ=255)                                                            ccGTTCAGCGGCAGAGGACCACCGATAACCGCACCATTATCGGAGGTAAAGAGAATAATTGTATTGTCATACTGTCCGTTTTTCTTCAgtt                  >  2:675490/1‑91 (MQ=255)                                                              gTTCAGCGGCAGAGGACCACCGATAACCGCACCATTATCGGAGGTAAAGAGAATAATTGTATTGTCATACTGTCCGTTTTTCTTCAgttgt                >  1:822111/1‑91 (MQ=255)                                                                      gCAGAGGACCACCGATAACCGCACCATTATCGGAGGTAAAGAGAATAATTGTATTGTCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAAt        >  1:576335/1‑91 (MQ=255)                                                                      gCAGAGGACCACCGATAACCGCACCATTATCGGAGGTAAAGAGAATAATTGTATTGTCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAAt        >  1:404588/1‑91 (MQ=255)                                                                        agagGACCACCGATAACCGCACCATTATCGGAGGTAAAGAGAATAATTGTATTGTCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGc      >  2:805696/1‑91 (MQ=255)                                                                            gACCACCGATAACCGCACCATTATCGGAGGTAAAGAGAATAATTGTATTGTCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGttt  <  1:564202/91‑1 (MQ=255)                                                                                 |                                                                                      TAAACATTGGGGTGTGAGTACCGCCAGGATAGGTCTGACTCTTATAGCCTTTTTGCGCCCCGTTCAGCGGCAGAGGACCATCGATAACCGCACCATTATCGGAGGTAAAGAGAATAATTGTATTGTCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTT  >  NZ_CP009273/1575650‑1575815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑