Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,578,188	A→G	I10T (ATC→ACC)	safA ←	two‑component system connector SafA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,578,188	0	A	G	100.0%	61.8 / NA	17	I10T (ATC→ACC)	safA	two‑component system connector SafA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/11);  total (6/11)

CAACGTAAGTGTCAGTAATAAGACAACCACAATTGCAGACATGATTTCTTTATAGTTGTCTCTTTGCGTGATTTTGTTTTTCACTGTGGTCGCATGCATTTCAAATATGTTTATTTAGCGGATAACGTTAAAAATCGTTAATCAGTATGCTT  >  NZ_CP009273/1578118‑1578269                                                                       |                                                                                  cAACGTAAGTGTCAGTAATAAGACAACCACAATTGCAGACATGATTTCTTTATAGTTGTCTCTTTGCGTGGTTTTGTTTTTCACTGTGGTc                                                               <  2:30284/91‑1 (MQ=255)       aaGTGTCAGTAATAAGACAACCACAATTGCAGACATGATTTCTTTATAGTTGTCTCTTTGCGTGGTTTTGTTTTTCACTGTGGTCgcatgc                                                         <  1:264074/91‑1 (MQ=255)              aGTAATAAGACAACCACAATTGCAGACATGATTTCTTTATAGTTGTCTCTTTGCGTGGTTTTGTTTTTCACTGTGGTCGCATGCATTTCaa                                                  <  1:621085/91‑1 (MQ=255)                               aaTTGCAGACATGATTTCTTTATAGTTGTCTCTTTGCGTGGTTTTGTTTTTc                                                                        >  2:284859/1‑52 (MQ=255)                               aaTTGCAGACATGATTTCTTTATAGTTGTCTCTTTGCGTGGTTTTGTTTTTc                                                                        <  1:284859/52‑1 (MQ=255)                                   gCAGACATGATTTCTTTATAGTTGTCTCTTTGCGTGGTTTTGTTTTTCACTGTGGTCGCATGCATTTCAAATATGTTTATTTAGCGGATaa                             <  1:812525/91‑1 (MQ=255)                                   gCAGACATGATTTCTTTATAGTTGTCTCTTTGCGTGGTTTTGTTTTTCACTGTGGTCGCATGCATTTCAAATATGTTTATTTAGCGGATaa                             <  2:224390/91‑1 (MQ=255)                                        cATGATTTCTTTATAGTTGTCTCTTTGCGTGGTTTTGTTTTTCACTGTGGTCGCATGCATTTCAAATATGTTTATTTAGCGGATAACGTTa                        >  1:121241/1‑91 (MQ=255)                                             tttCTTTATAGTTGTCTCTTTGCGTGGTTTTGTTTTTCACTGTGGTCGCATGCATTTCAAATATGTTTATTTAGCGGATAACGTTAAAAAt                   >  1:344880/1‑91 (MQ=255)                                                  ttATAGTTGTCTCTTTGCGTGGTTTTGTTTTTCACTGTGGTCGCATGCATTTCAAATATGTTTATTTAGCGGATAACGTTAAAAATCGTTa              >  1:522352/1‑91 (MQ=255)                                                  ttATAGTTGTCTCTTTGCGTGGTTTTGTTTTTCACTGTGGTCGCATGCATTTCAAATATGTTTATTTAGCGGATAACGTTAAAAATCGTTa              >  1:718519/1‑91 (MQ=255)                                                  ttATAGTTGTCTCTTTGCGTGGTTTTGTTTTTCACTGTGGTCGCATGCATTTCAAATATGTTTATTTAGCGGATAACGTTAAAAATCGTTa              <  2:121241/91‑1 (MQ=255)                                                         tGTCTCTTTGCGTGGTTTTGTTTTTCACTGTGGTCGCATGCATTTCAAATATGTTTATTTAGCGGATAACGTTAAAAATCGTTAATCAGTa       >  1:506086/1‑91 (MQ=255)                                                         tGTCTCTTTGCGTGGTTTTGTTTTTCACTGTGGTCGCATGCATTTCAAATATGTTTATTTAGCGGATAACGTTAAAAATCGTTAATCAGTa       <  2:344880/91‑1 (MQ=255)                                                            ctctTTGCGTGGTTTTGTTTTTCACTGTGGTCGCATGCATTTCAAATATGTTTATTTAGCGGATAACGTTAAAAATCGTTAATCAGTATGc    <  1:540936/91‑1 (MQ=255)                                                              ctTTGCGTGGTTTTGTTTTTCACTGTGGTCGCATGCATTTCAAATATGTTTATTTAGCGGATAACGTTAAAAATCGTTAATCAGTATGCtt  <  1:584127/91‑1 (MQ=255)                                                              ctTTGCGTGGTTTTGTTTTTCACTGTGGTCGCATGCATTTCAAATATGTTTATTTAGCGGATAACGTTAAAAATCGTTAATCAGTATGCtt  <  2:522352/91‑1 (MQ=255)                                                                       |                                                                                  CAACGTAAGTGTCAGTAATAAGACAACCACAATTGCAGACATGATTTCTTTATAGTTGTCTCTTTGCGTGATTTTGTTTTTCACTGTGGTCGCATGCATTTCAAATATGTTTATTTAGCGGATAACGTTAAAAATCGTTAATCAGTATGCTT  >  NZ_CP009273/1578118‑1578269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑