Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	204,632	T→C	V1007A (GTC→GCC)	dnaE →	DNA polymerase III subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	204,632	0	T	C	100.0%	80.6 / NA	23	V1007A (GTC→GCC)	dnaE	DNA polymerase III subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/9);  total (14/9)

GGCGTAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCT  >  NZ_CP009273/204547‑204716                                                                                      |                                                                                     ggCGTAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGccacca                                                                                 >  1:652382/1‑91 (MQ=255)     tAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAgcg                                                                             >  1:31227/1‑91 (MQ=255)           tGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCaa                                                                       <  1:204218/91‑1 (MQ=255)                  cATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATc                                                                >  1:275002/1‑91 (MQ=255)                  cATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATc                                                                >  2:434530/1‑91 (MQ=255)                  cATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATc                                                                <  1:602730/91‑1 (MQ=255)                  cATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATc                                                                <  1:768247/91‑1 (MQ=255)                          cGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTg                                                        >  2:481792/1‑91 (MQ=255)                               gAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGc                                                   >  1:495877/1‑91 (MQ=255)                                    tGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGAt                                              >  2:842742/1‑91 (MQ=255)                                         aaGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCg                                         >  2:137290/1‑91 (MQ=255)                                             catcaCGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTcc                                     >  1:834630/1‑91 (MQ=255)                                             catcaCGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTcc                                     >  1:775112/1‑91 (MQ=255)                                              atcaCGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCg                                    >  1:672347/1‑91 (MQ=255)                                                        cGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGa                          <  2:556003/91‑1 (MQ=255)                                                             cTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGa                     <  1:597801/91‑1 (MQ=255)                                                             cTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGa                     <  2:5240/91‑1 (MQ=255)                                                             cTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGa                     <  2:652382/91‑1 (MQ=255)                                                                gttgttGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGAtgt                  <  1:516574/91‑1 (MQ=255)                                                                   attgCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGAtgttgt               >  2:293900/2‑91 (MQ=255)                                                                     tgCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGGATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGGAGTGATGTTGttt             >  2:21276/1‑91 (MQ=255)                                                                      gCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTa            >  1:231998/1‑91 (MQ=255)                                                                           gcgGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGAc       >  2:117068/1‑91 (MQ=255)                                                                                ttATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCt  <  2:672347/91‑1 (MQ=255)                                                                                      |                                                                                     GGCGTAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCT  >  NZ_CP009273/204547‑204716

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑