Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,578,845	A→G	D633D (GAT→GAC)	ydeP ←	acid resistance putative oxidoreductase YdeP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,578,845	0	A	G	100.0%	213.1 / NA	56	D633D (GAT→GAC)	ydeP	acid resistance putative oxidoreductase YdeP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/18);  total (38/18)

GATCATCCATACCATAAATCGTCGTGTTGTACTGATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGATCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAGCGCCTTTCAGCAGCTGCATTTATCA  >  NZ_CP009273/1578757‑1578927                                                                                         |                                                                                   gATCATCCATACCATAAATCGTCGTGTTGTACTGATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGtc                                                                                  >  2:468798/1‑91 (MQ=255) gATCATCCATACCATAAATCGTCGTGTTGTACTGATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGtc                                                                                  <  2:852978/91‑1 (MQ=255) gATCATCCATACCATAAATCGTCGTGTTGTACTGATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGtc                                                                                  >  2:630175/1‑91 (MQ=255)  atcatcCATACCATAAATCGTCGTGTTGTACTGATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGtct                                                                                 <  1:503769/91‑1 (MQ=255)      tcCATACCATAAATCGTCGTGTTGTACTGATCGTGGCAGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTa                                                                             >  1:802585/1‑91 (MQ=255)        cataccataAATCGTCGTGTTGTACTGATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAAc                                                                           <  2:565091/91‑1 (MQ=255)        cataccataAATCGTCGTGTTGTACTGATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAAc                                                                           <  2:310523/91‑1 (MQ=255)              ataAATCGTCGTGTTGTACTGATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCt                                                                     >  1:129109/1‑91 (MQ=255)              ataAATCGTCGTGTTGTACTGATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCt                                                                     >  2:3162/1‑91 (MQ=255)              ataAATCGTCGTGTTGTACTGATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCt                                                                     >  2:249747/1‑91 (MQ=255)              ataAATCGTCGTGTTGTACTGATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCt                                                                     >  2:698064/1‑91 (MQ=255)              ataAATCGTCGTGTTGTACTGATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCt                                                                     >  1:524667/1‑91 (MQ=255)                aaaTCGTCGTGTTGTACTGATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCttt                                                                   >  2:477055/1‑91 (MQ=255)                aaaTCGTCGTGTTGTACTGATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCttt                                                                   >  1:798477/1‑91 (MQ=255)                    cgtcgtGTTGTACTGATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTg                                                               >  2:310425/1‑91 (MQ=255)                    cgtcgtGTTGTACTGATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTg                                                               <  2:129109/91‑1 (MQ=255)                      tcgtGTTGTACTGATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGt                                                             >  1:378156/1‑91 (MQ=255)                        gtgtTGTACTGATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTaa                                                           >  2:592458/1‑91 (MQ=255)                         tgttgtACTGATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAAt                                                          <  2:374985/91‑1 (MQ=255)                             gtACTGATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGaaa                                                      >  1:527645/1‑91 (MQ=255)                              tACTGATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAAt                                                     <  1:310425/91‑1 (MQ=255)                               aCTGATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAAtt                                                    <  2:679209/91‑1 (MQ=255)                               aCTGATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAAtt                                                    >  1:176763/1‑91 (MQ=255)                                  gATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGtctt                                                                                >  1:74497/1‑60 (MQ=255)                                  gATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGtctt                                                                                <  2:74497/60‑1 (MQ=255)                                  gATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCGTTTGCTGGTAATGAAAttgac                                                 >  2:703730/1‑88 (MQ=255)                                  gATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCt                                                                <  1:383181/76‑1 (MQ=255)                                  gATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCt                                                                >  2:383181/1‑76 (MQ=255)                                  gATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAAt                                                     >  2:754417/1‑87 (MQ=255)                                  gATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAAt                                                     <  1:754417/87‑1 (MQ=255)                                  gATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGc                                                 >  2:159406/1‑91 (MQ=255)                                  gATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGc                                                 >  2:23259/1‑91 (MQ=255)                                  gATCGTGGCTGCGCACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACATCCCTTTGCGGGTAATGAAATTAGc                                                 >  2:530527/1‑91 (MQ=255)                                   aTCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAAGAGCCCTTTGCTGGTAATGAAATTAGcc                                                >  1:527969/1‑91 (MQ=255)                                    tCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCt                                               >  2:267169/1‑91 (MQ=255)                                       tGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTAc                                            >  2:868766/1‑91 (MQ=255)                                       tGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTAc                                            >  2:376226/1‑91 (MQ=255)                                       tGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTAc                                            >  2:178304/1‑91 (MQ=255)                                       tGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTAc                                            >  2:430739/1‑91 (MQ=255)                                       cggCTGCGTACTGTCGCCATGACCAGCTTTCTGTTAAACGCTGCAGAGGGGCCTTCTCACAGCCCTTTGCTGGTAATGAAATTAGCCTTAc                                            >  2:86972/2‑91 (MQ=255)                                            gCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGAc                                       <  2:524667/91‑1 (MQ=255)                                            gCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGAc                                       >  1:364594/1‑91 (MQ=255)                                            gCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGAc                                       >  2:371776/1‑91 (MQ=255)                                            gCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGAc                                       <  1:468798/91‑1 (MQ=255)                                             cGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACg                                      >  1:284679/1‑91 (MQ=255)                                              gTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACgg                                     >  2:767802/1‑91 (MQ=255)                                                 cTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCtt                                                                    >  1:133882/1‑57 (MQ=255)                                                 cTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCtt                                                                    <  2:133882/57‑1 (MQ=255)                                                         aTGACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAgc                          <  2:401333/91‑1 (MQ=255)                                                           gACCAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGATGGCGTCATCCAgcgc                        >  2:231958/1‑91 (MQ=255)                                                             ccAGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAGCGCCt                      >  2:375772/1‑91 (MQ=255)                                                               aGCTTACTGTTAAACGCTGAAGAGGGGTCTTCTACCAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAGCGCCttt                    >  1:801126/1‑91 (MQ=255)                                                                 cTTACTGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAGCGCCTTTca                  <  1:698064/91‑1 (MQ=255)                                                                      tGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAGCGCCTTTCagcagc             <  2:622624/91‑1 (MQ=255)                                                                      tGTTAAACGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAGCGCCTTTCagcagc             <  1:630175/91‑1 (MQ=255)                                                                          aaaCGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTCCCTGACGGCGTCATCCAGCGCCTTTCCGCAGCTGCa         >  2:16183/1‑91 (MQ=255)                                                                          aaaCGCTGAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAGCGCCTTTCAGCAGCTGCa         >  2:527810/1‑91 (MQ=255)                                                                                 gAAGAGGGGTCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAGCGCCTTTCAGCAGCTGCATTTATCa  >  2:578373/1‑91 (MQ=255)                                                                                         |                                                                                   GATCATCCATACCATAAATCGTCGTGTTGTACTGATCGTGGCTGCGTACTGTCGCCATGACCAGCTTACTGTTAAACGCTGAAGAGGGATCTTCTAACAGCCCTTTGCTGGTAATGAAATTAGCCTTACCTGACGGCGTCATCCAGCGCCTTTCAGCAGCTGCATTTATCA  >  NZ_CP009273/1578757‑1578927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑