Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,580,214	T→C	D177G (GAC→GGC)	ydeP ←	acid resistance putative oxidoreductase YdeP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,580,214	0	T	C	100.0%	118.7 / NA	32	D177G (GAC→GGC)	ydeP	acid resistance putative oxidoreductase YdeP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/16);  total (16/16)

AAAGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGAAG  >  NZ_CP009273/1580126‑1580292                                                                                         |                                                                               aaaGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCg                                                                              <  1:563708/91‑1 (MQ=255)      cTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGaaa                                                                         <  1:220693/91‑1 (MQ=255)       ttCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAg                                                                        >  2:781717/1‑91 (MQ=255)       ttCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAg                                                                        >  1:578814/1‑91 (MQ=255)              aaCACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGc                                                                 <  2:800405/91‑1 (MQ=255)              aaCACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGc                                                                 <  2:436029/91‑1 (MQ=255)              aaCACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGc                                                                 <  2:415581/91‑1 (MQ=255)                           tACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACg                                                    <  1:467432/91‑1 (MQ=255)                            aCCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACgg                                                   >  1:423140/1‑91 (MQ=255)                              cTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACGGGc                                                 >  2:81954/1‑91 (MQ=255)                              cTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACAGGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACGGGc                                                 >  1:815429/1‑91 (MQ=255)                                acacCGATACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACGGGCaa                                               >  2:342898/1‑91 (MQ=255)                                    cGATACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAg                                           >  2:745302/1‑91 (MQ=255)                                    cGATACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAg                                           >  2:585401/1‑91 (MQ=255)                                     gATACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGc                                          >  1:150899/1‑91 (MQ=255)                                      aTACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAACt                                         >  2:833684/1‑91 (MQ=255)                                         cTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGAt                                      <  1:353795/91‑1 (MQ=255)                                           cGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATaa                                    >  1:752671/1‑91 (MQ=255)                                           cGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATaa                                    >  1:416873/1‑91 (MQ=255)                                              tGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATaaaga                                 <  1:576408/91‑1 (MQ=255)                                               gCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATaaagaa                                >  1:367108/1‑91 (MQ=255)                                                    aCCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCa                           <  2:466627/91‑1 (MQ=255)                                                     cccACGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAg                          <  2:723631/91‑1 (MQ=255)                                                         cGCTTGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTc                      <  1:639990/91‑1 (MQ=255)                                                             tGTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTg                  >  1:535019/1‑91 (MQ=255)                                                              gTCGGTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTgg                 >  2:570685/1‑91 (MQ=255)                                                                  gTTCATGGCACATGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGt             >  1:834885/1‑91 (MQ=255)                                                                           acaTGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGa    <  1:781717/91‑1 (MQ=255)                                                                           acaTGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGa    <  2:436879/91‑1 (MQ=255)                                                                           acaTGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGa    <  2:480780/91‑1 (MQ=255)                                                                           acaTGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGa    <  1:570685/91‑1 (MQ=255)                                                                             aTGTTGGAGCAGCCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGAAg  <  2:648278/91‑1 (MQ=255)                                                                                         |                                                                               AAAGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGAAG  >  NZ_CP009273/1580126‑1580292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑