Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,613,590	A→C	T72P (ACC→CCC)	marR →	multiple antibiotic resistance transcriptional regulator MarR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,613,590	0	A	C	100.0%	92.6 / NA	25	T72P (ACC→CCC)	marR	multiple antibiotic resistance transcriptional regulator MarR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (14/11);  total (14/11)

GTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTT  >  NZ_CP009273/1613502‑1613676                                                                                         |                                                                                       gTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGccc                                                                                      <  1:85356/91‑1 (MQ=255)   ttAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGCCCCg                                                                                    <  2:450547/91‑1 (MQ=255)   ttAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGCCCCg                                                                                    <  2:410161/91‑1 (MQ=255)           ctctgctctATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGCCCCGTATGCTgg                                                                            >  2:454920/1‑91 (MQ=255)           ctctgctctATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGCCCCGTATGCTgg                                                                            >  2:281912/1‑91 (MQ=255)                 tctATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGCCCCGTATGCTGGATCGcc                                                                      <  1:281912/91‑1 (MQ=255)                 tctATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGCCCCGTATGCTGGATCGcc                                                                      <  2:651710/91‑1 (MQ=255)                 tctATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGCCCCGTATGCTGGATCGcc                                                                      <  2:640964/91‑1 (MQ=255)                      ccGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGCCCCGTATGCTGGATCGCCTGGTc                                                                 <  1:389512/91‑1 (MQ=255)                       cGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGCCCCGTATGCTGGATCGcc                                                                      >  2:795660/1‑85 (MQ=255)                       cGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGCCCCGTATGCTGGATCGcc                                                                      <  1:795660/85‑1 (MQ=255)                         cTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGCCCCGTATGCTGGATCGCCTGGTCTGt                                                              >  1:351166/1‑91 (MQ=255)                              cggcgTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGCCCCGTATGCTGGATCGCCTGGTCTGTAAAgg                                                         >  2:447258/1‑91 (MQ=255)                               ggcgTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGCCCCGTATGCTGGATCGCCTGGTCTGTAAAGGc                                                        <  1:285676/91‑1 (MQ=255)                                gcgTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGCCCCGTATGCTGGATCGCCTGGTCTGTAAAGGCt                                                       <  1:465030/91‑1 (MQ=255)                                              ggTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGCCCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTg                                         >  2:378578/1‑91 (MQ=255)                                              ggTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGCCCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTg                                         >  1:251572/1‑91 (MQ=255)                                                               tATTGTCGGTCGACCTGGGAGCACTGCCCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACaa                        >  1:778118/1‑91 (MQ=255)                                                                   gtcggtcgACCTGGGAGCACTGCCCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAgcgc                    <  1:422622/91‑1 (MQ=255)                                                                   gtcggtcgACCTGGGAGCACTGCCCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAgcgc                    >  2:463724/1‑91 (MQ=255)                                                                   gtcggtcgACCTGGGAGCACTGCCCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAgcgc                    >  2:643128/1‑91 (MQ=255)                                                                   gtcggtcgACCTGGGAGCACTGCCCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAgcgc                    >  1:639690/1‑91 (MQ=255)                                                                   gtcggtcgACCTGGGAGCACTGCCCCGTATGCTGCATCGCCTCGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGACCCCGAATGACAAgcgc                    <  2:356518/91‑1 (MQ=255)                                                                         cgACCTGGGAGCACTGCCCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTa              <  1:463724/91‑1 (MQ=255)                                                                                     aCTGCCCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACtt  >  1:523502/1‑91 (MQ=255)                                                                                     aCTGCCCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACtt  >  2:174674/1‑91 (MQ=255)                                                                                     aCTGCCCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACtt  >  1:733337/1‑91 (MQ=255)                                                                                         |                                                                                       GTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTT  >  NZ_CP009273/1613502‑1613676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑