Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,622,039	A→G	E89G (GAG→GGG)	ydfG →	bifunctional NADP‑dependent 3‑hydroxy acid dehydrogenase/3‑hydroxypropionate dehydrogenase YdfG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,622,039	0	A	G	100.0%	136.8 / NA	37	E89G (GAG→GGG)	ydfG	bifunctional NADP‑dependent 3‑hydroxy acid dehydrogenase/3‑hydroxypropionate dehydrogenase YdfG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/19);  total (18/19)

TGCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGAGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACAAAGGCCTGGTATATATGACGCGCGCCGTCT  >  NZ_CP009273/1621964‑1622125                                                                            |                                                                                       tGCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGc                                                                         >  1:761891/1‑91 (MQ=255) tGCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGc                                                                         >  1:477830/1‑91 (MQ=255)    tGGCATCGCTTCCTGCCGAGTGGTGCAATATTGATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAg                                                                      <  2:282704/91‑1 (MQ=255)         tCGCTTCCTGCCGAGTGGTGCAATATTGATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTg                                                                 <  2:477830/91‑1 (MQ=255)                    cGAGTGGTGCAATATTGATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGaa                                                      <  2:626849/91‑1 (MQ=255)                     gAGTGGTGCAATATTGATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGaaa                                                     >  2:11371/1‑91 (MQ=255)                                 atTGATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATa                                         <  1:784840/91‑1 (MQ=255)                                    gATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACca                                      >  1:1557/1‑91 (MQ=255)                                    gATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACca                                      >  1:44807/1‑91 (MQ=255)                                    gATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACca                                      >  1:180762/1‑91 (MQ=255)                                         ccTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTgc                                                                                  <  1:157190/42‑1 (MQ=255)                                         ccTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTgc                                                                                  >  2:157190/1‑42 (MQ=255)                                         ccTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACcaacaac                                 >  1:493914/1‑91 (MQ=255)                                         ccTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACcaacaac                                 >  2:235902/1‑91 (MQ=255)                                         ccTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACcaacaac                                 >  1:864081/1‑91 (MQ=255)                                           tGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTg                                                                 <  1:111091/57‑1 (MQ=255)                                           tGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTg                                                                 >  2:111091/1‑57 (MQ=255)                                           tGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACcaacaacaa                               >  2:447500/1‑91 (MQ=255)                                           tGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACcaacaacaa                               >  2:514283/1‑91 (MQ=255)                                           tGGTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACcaacaacaa                               <  1:372594/91‑1 (MQ=255)                                            ggTAAATAATGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACaaa                              <  2:422257/91‑1 (MQ=255)                                                aataatGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACAAAGGcc                          <  1:311193/91‑1 (MQ=255)                                                aataatGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACAAAGGcc                          <  2:864081/91‑1 (MQ=255)                                                aataatGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACAAAGGcc                          <  1:514283/91‑1 (MQ=255)                                                aataatGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACAAAGGcc                          <  2:324243/91‑1 (MQ=255)                                                aataatGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACAAAGGcc                          <  2:378540/91‑1 (MQ=255)                                                   aatGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACAAAGGCCTgg                       >  1:441174/1‑91 (MQ=255)                                                    atGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACAAAGGCCTGGt                      <  2:262299/91‑1 (MQ=255)                                                     tGCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACAAAGGCCTGGta                     >  2:624118/1‑91 (MQ=255)                                                      gCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACAAAGGcc                          >  2:94368/1‑85 (MQ=255)                                                      gCCGGCCTGGCGTTGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACAAAGGcc                          <  1:94368/85‑1 (MQ=255)                                                                   tGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACAAAGGCCTGGTATATATGACgcgcgc       <  2:199082/91‑1 (MQ=255)                                                                   tGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACAAAGGCCTGGTATATATGACgcgcgc       <  1:807435/91‑1 (MQ=255)                                                                   tGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACAAAGGCCTGGTATATATGACgcgcgc       <  1:569380/91‑1 (MQ=255)                                                                   tGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACAAAGGCCTGGTATATATGACgcgcgc       >  2:807435/1‑91 (MQ=255)                                                                   tGGGCATGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACAAAGGCCTGGTATATATGACgcgcgc       <  1:558235/91‑1 (MQ=255)                                                                        aTGGGGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACAAAGGCCTGGTATATATGACGCGCGCCGTCt  >  1:331902/1‑91 (MQ=255)                                                                            |                                                                                       TGCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGATATCCTGGTAAATAATGCCGGCCTGGCGTTGGGCATGGAGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAACAAAGGCCTGGTATATATGACGCGCGCCGTCT  >  NZ_CP009273/1621964‑1622125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑