Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,701,793	A→C	P206P (CCA→CCC)	rsxC →	electron transport complex subunit RsxC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,701,793	0	A	C	100.0%	94.3 / NA	26	P206P (CCA→CCC)	rsxC	electron transport complex subunit RsxC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (14/12);  total (14/12)

GTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCACGCGAAATTCTTATCGGCATTGAAGATAACAAACCGCAGGCGATTTCCATGCTGCGCGCGGTGCTGGCGGACTCTAACGATATTTCT  >  NZ_CP009273/1701706‑1701880                                                                                        |                                                                                        gTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCCcgc                                                                                      >  2:255603/1‑91 (MQ=255)  tACATTACCGCCGATGACCGTTTGATGAAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCCcgcg                                                                                     >  2:495808/1‑91 (MQ=255)        aCCGCCGATGACCGCTTGATGCAGGATTGCGCGGCTCAGGCCGTAGAGGGGATTCGCATTCTTGCGCATATTCTGCAGCCCCGCGAAATTc                                                                               >  2:302345/1‑91 (MQ=255)         ccgccgATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCCCGCGAAATTCt                                                                              >  1:845916/1‑91 (MQ=255)           gccgATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCCCGCGAAATTCTTa                                                                            >  1:247404/1‑91 (MQ=255)           gccgATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCCCGCGAAATTCTTa                                                                            >  2:51271/1‑91 (MQ=255)             cgATGACCGGTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCCCGCGAAATTCTTATc                                                                          <  1:117557/91‑1 (MQ=255)               aTGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCCCGCGAAATTCTTATCgg                                                                        <  1:240575/91‑1 (MQ=255)               aTGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCCCGCGAAATTCTTATCgg                                                                        <  1:638507/91‑1 (MQ=255)                  gccGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCCCGCGAAATTCTTATCGGCAt                                                                     <  2:831986/90‑1 (MQ=255)                     gTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCCCGCGAAATTCTTATCGGCATTGa                                                                  >  2:434611/1‑91 (MQ=255)                      tttGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCCCGCGAAATTCTTATCGGCATTGaa                                                                 <  2:165114/91‑1 (MQ=255)                             cAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCCCGCGAAATTCTTATCGGCATTGAAGATAACa                                                          >  2:38251/1‑91 (MQ=255)                                        ggCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCCCGCGAAATTCTTATCGGCATTGAAGATAACAAACCGCAGGCg                                               <  2:633762/91‑1 (MQ=255)                                                   tAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCCCGCGAAATTCTTATCGGCATTGAAGATAACAAACCGCAGGCGATTTCCAtgct                                    >  2:866428/1‑91 (MQ=255)                                                   tAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCCCGCGAAATTCTTATCGGCATTGAAGATAACAAACCGCAGGCGATTTCCAtgct                                    >  2:792776/1‑91 (MQ=255)                                                         gTATTTGCATTCTTGCGCATATTCTGCAGCCCCGCGAAATTCTTATCGGCATTGAAGATAACAAACCGCAGGCGATTTCCATGCTgcgcgc                              <  2:127103/91‑1 (MQ=255)                                                         gTATTCGCATTCTTGCGCATATTCTGCAGCCCCGCGAAATTCTTATCGGCATTGAAGATAACAAACCGCAGGCGATTTCCATGCTgcgcgc                              <  1:274572/91‑1 (MQ=255)                                                         gTATTCGCATTCTTGCGCATATTCTGCAGCCCCGCGAAATTCTTATCGGCATTGAAGATAACAAACCGCAGGCGATTTCCATGCTgcgcgc                              <  1:255603/91‑1 (MQ=255)                                                          tATTCGCATTCTTGCGCATATTCTGCAGCCCCGCGAAATTCTTATCGGCATTGAAGATAACAAACCGCAGGCGATTTCCATGCTGcgcgcg                             >  1:609598/1‑91 (MQ=255)                                                                    cTTGCGCATATTCTGCAGCCCCGCGAAATTCTTATCGGCATTGAAGATAACAAACCGCAGGCGATTTCCATGCTGCGCGCGGTGCTGGCgg                   <  1:373005/91‑1 (MQ=255)                                                                          cATATTCTGCAGCCCCGCGAAATTCTTATCGGCATTGAAGATAACAAACCGCAGGCGATTTCCATGCTGCGCGCGGTGCTGGCGGACTCTa             <  2:486643/91‑1 (MQ=255)                                                                          cATATTCTGCAGCCCCGCGAAATTCTTATCGGCATTGAAGATAACAAACCGCAGGCGATTTCCATGCTGCGCGCGGTGCTGGCGGACTCTa             <  2:609598/91‑1 (MQ=255)                                                                            tatTCTGCAGCCCCGCGAAATTCTTATCGGCATTGAAGATAACAAACCGCAGGCGATTTCCATGCTGCGCGCGGTGCTGGCGGACTCTAAc           >  2:525917/1‑91 (MQ=255)                                                                                cTGCAGCCCCGCGAAATTCTTATCGGCATTGAAGATAACAAACCGCAGGCGATTTCCATGCTGCGCGCGGTGCTGGCGGACTCTAACGata       >  1:185159/1‑91 (MQ=255)                                                                                    aGCCCCGCGAAATTCTTATCGGCATTGAAGATAACAAACCGCAGGCGATTTCCATGCTGCGCGCGGTGCTGGCGGACTCTAACGATATTtc   >  1:756628/1‑91 (MQ=255)                                                                                     gCCCCGCGAAATTCTTATCGGCATTGAAGATAACAAACCGCAGGCGATTTCCATGCTGCGCGCGGTGCTGGCGGACTCTAACGATATTtct  >  2:413988/1‑91 (MQ=255)                                                                                        |                                                                                        GTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCACGCGAAATTCTTATCGGCATTGAAGATAACAAACCGCAGGCGATTTCCATGCTGCGCGCGGTGCTGGCGGACTCTAACGATATTTCT  >  NZ_CP009273/1701706‑1701880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑