Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,708,538	A→G	intergenic (+10/‑96)	dtpA → / → gstA	dipeptide/tripeptide permease DtpA/glutathione transferase GstA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,708,538	0	A	G	100.0%	56.0 / NA	19	intergenic (+10/‑96)	dtpA/gstA	dipeptide/tripeptide permease DtpA/glutathione transferase GstA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/7);  total (12/7)

TGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAG  >  NZ_CP009273/1708452‑1708617                                                                                       |                                                                                tgctgACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGGGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAAct                                                                             >  2:743049/1‑91 (MQ=255) tgctgACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAAct                                                                             >  2:258655/1‑91 (MQ=255)    tgACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCtt                                                                          >  1:170974/1‑91 (MQ=255)      aCCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCtttt                                                                        >  1:494895/1‑91 (MQ=255)         gcgcCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACa                                                                     >  1:212427/1‑91 (MQ=255)                    gCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCGCTAACt                                                          >  1:140395/1‑91 (MQ=255)                      aCCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCGCTAACttt                                                        >  1:698925/1‑91 (MQ=255)                         gCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCGCTAACTTTTCg                                                     >  2:473719/1‑91 (MQ=255)                              aCGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCGCTAACTTTTCGTTAGc                                                >  2:154498/1‑91 (MQ=255)                                        aCGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCttttttt                                      >  2:740852/1‑91 (MQ=255)                                         cGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCtttttttt                                     <  2:140395/91‑1 (MQ=255)                                             gCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGtt                                 >  2:469067/1‑91 (MQ=255)                                             gCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGtt                                 >  2:406522/1‑91 (MQ=255)                                                      ggggcgAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGc                        <  1:529386/89‑1 (MQ=255)                                                      gcggcgAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGc                        <  1:792808/91‑1 (MQ=255)                                                      gaggcgAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGc                        <  1:80744/89‑1 (MQ=255)                                                        ggcaAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCAc                      <  1:611916/91‑1 (MQ=255)                                                        ggcaAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCAc                      <  1:547477/91‑1 (MQ=255)                                                                          gTAAAATTCAGGGGACCTTTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACcac    <  2:447237/91‑1 (MQ=255)                                                                          gTAAAATTCAGGGGACCTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACcac    <  2:819278/91‑1 (MQ=255)                                                                          gTAAAATTCAGGGGAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACcac    <  1:354324/91‑1 (MQ=255)                                                                            aaaaTTCAGGGGAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAg  <  1:473719/91‑1 (MQ=255)                                                                                       |                                                                                TGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAG  >  NZ_CP009273/1708452‑1708617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑