Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,732,351	(A)7→6	coding (251/1026 nt)	purR →	HTH‑type transcriptional repressor PurR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,732,345	0	A	.	100.0%	57.3 / NA	14	coding (245/1026 nt)	purR	HTH‑type transcriptional repressor PurR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (7/7);  total (7/7)

CCCCTAGCGCGGTGGCGCGTAGCCTGAAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTGAAAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGTCTGCTGGTGA  >  NZ_CP009273/1732237‑1732464                                                                                                             |                                                                                                                        ccccTAGCGCGGTGGCGCGTAGCCTGAAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCt                                                                                           >  1:286594/1‑138 (MQ=255)       gcgcGGTGGCGCGTAGCCTGAAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTg                                                                                    >  2:287886/1‑139 (MQ=255)           ggTGGCGCGTAGCCTGAAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCa                                                                                <  2:166854/139‑1 (MQ=255)                                tAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTgag                                                            >  2:52600/1‑138 (MQ=255)                                 aaCCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCa                                                                                <  2:381014/117‑1 (MQ=255)                                 aaCCACACCAAGTCTATCGGTTTGCTGGCGAACAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCa                                                                                >  1:381014/1‑117 (MQ=255)                                     acacCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAg                                                      <  2:313291/139‑1 (MQ=255)                                       acCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCg                                                    >  2:210323/1‑139 (MQ=255)                                                 tCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTAt                                             <  1:113689/136‑1 (MQ=255)                                                 tCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTAt                                             <  2:401939/136‑1 (MQ=255)                                                               aCCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGgcgc                             >  1:59868/1‑138 (MQ=255)                                                                              gCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATgg             <  1:287886/139‑1 (MQ=255)                                                                                cTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGTc           <  2:9993/139‑1 (MQ=255)                                                                                         cGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGTCTGCTGGTGa  >  1:413389/1‑139 (MQ=255)                                                                                                             |                                                                                                                        CCCCTAGCGCGGTGGCGCGTAGCCTGAAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTGAAAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGTCTGCTGGTGA  >  NZ_CP009273/1732237‑1732464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑