Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,891,886	Δ1 bp	coding (698/1365 nt)	sdaA →	L‑serine ammonia‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,891,884	0	C	.	100.0%	65.4 / NA	16	coding (696/1365 nt)	sdaA	L‑serine ammonia‑lyase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (10/6);  total (10/6)

TCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGCGATAAACTGTCTAACGATCCGATGAATGTCATTGACTGGGTAAACATGTTTGCGCTGGCAGTTAACG  >  NZ_CP009273/1891758‑1892005                                                                                                                               |                                                                                                                          tctcGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGG‑CCGCTGCGCGTGc                                                                                                              <  2:99639/139‑1 (MQ=255)      cTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGG‑CCGCTGCGCGTGCCAcgt                                                                                                         >  2:104204/1‑139 (MQ=255)      cTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGG‑CCGCTGCGCGTGCCAcgt                                                                                                         >  2:374636/1‑139 (MQ=255)         tGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGG‑CCGCTGCGCGTGCCAcgtcg                                                                                                       <  1:104204/138‑1 (MQ=255)                                  cAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGGGTACTGCAAGG‑CGGCGGCGCGGGCCACGTCGGGCGTCTGCCCTGCGCCGGATgtggg                                                                             >  2:206195/1‑135 (MQ=255)                                          agaTCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGG‑CCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGc                                                                     >  2:66004/1‑139 (MQ=255)                                            aTCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGG‑CCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGCGa                                                                   >  2:252329/1‑139 (MQ=255)                                                acgaGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGG‑CCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGCGATaaa                                                               <  1:374636/139‑1 (MQ=255)                                                            cgcATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGG‑CCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGCGATAAACTGTCTAACGAt                                                   <  1:66004/139‑1 (MQ=255)                                                             ccATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGG‑CCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGCGATAAACTGTCTAACGATc                                                  <  1:206195/138‑1 (MQ=255)                                                                             aTGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGG‑CCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGCGATAAACTGTCTAACGATCCGATGAATGTCATTGa                                  <  1:284094/139‑1 (MQ=255)                                                                                     aTGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGG‑CCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGCGATAAACTGTCTAACGATCCGATGAATGTCATTGACTGGGTa                           >  2:109296/1‑138 (MQ=255)                                                                                             atcgCGGGATGAACACCGAAGGTGTACTGCCAGG‑CCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGCGATAAACTGTCTAACGATCCGATGAATGTCATTGACTGGGTAAACATGttt                  >  2:423868/1‑139 (MQ=255)                                                                                             atcgCGGGATGAACACCGAAGGTGAACTGCCAGG‑CCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGCGATAAACTGTCTAACGATCCGATGAATGTCATTGACTGGGTAAACATGttt                  >  2:437321/1‑139 (MQ=255)                                                                                                 cgGGATGAACACCGAAGGTGTACTGCCAGG‑CCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGCGATAAACTGTCTAACGATCCGATGAATGTCATTGACTGGGTAAACATGTTTgcgc              >  2:160969/1‑139 (MQ=255)                                                                                                              gAAGGTGTACTGCCAGG‑CCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGCGATAAACTGTCTAACGATCCGATGAATGTCATTGACTGGGTAAACATGTTTGCGCTGGCAGTTAACg  >  2:239315/1‑138 (MQ=255)                                                                                                                               |                                                                                                                          TCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGCAAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCGTCTGCCCTGCGCCGGATGCTGGTTTCCAGCGATAAACTGTCTAACGATCCGATGAATGTCATTGACTGGGTAAACATGTTTGCGCTGGCAGTTAACG  >  NZ_CP009273/1891758‑1892005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑