| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,000,104 | 0 | T | A | 58.8% | ‑2.0 / 18.2 | 17 | N243K (AAT→AAA) | ycbF | fimbrial chaperone |
| Reads supporting (aligned to +/- strand): ref base T (0/7); new base A (10/0); total (10/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.14e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAAC‑AGGTTCGGAAAAC‑GTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACT > NZ_CP009273/999969‑1000230 | aGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTGAGTGGAAAGAAAGAGCAAAAACTg > 1:295711/1‑139 (MQ=255) tAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTGAGGGGAAAGAAGGAGGAAAAAATGccccg > 1:56526/1‑137 (MQ=255) tgtgGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTGGGGGGAAAGAAAGAGGAAAAACTTCCCTGAAAC‑aag > 1:253725/1‑137 (MQ=255) gtgCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTTGGTGGCAAAAAGGAGCAAAAACTGCCCTGAAAC‑AAGGCCGGGAAAc > 1:155237/1‑139 (MQ=255) gCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTTGGGGGAAAAAAGGAGAAAAAAATGCCCCGAAAA‑AAGGTCGGAAAAC‑GATTGCGgttttttttt > 1:332594/1‑137 (MQ=255) gCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTGGGGGCAAAGAAGGAGGAAAAACTGCCCCGAAACAAGGTTGGGAAACA‑GTTGGGGTTTTTTTT‑TCGCAGGGCaaa > 2:434901/1‑138 (MQ=255) aGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTTGGGGGAAAGAAAGAGGAAAAAACGCCCCGAAAC‑AAGGTTGGAAAACAGTTTCGGTTTTTTTTTCC‑CAGGGCAAATACCttttt > 1:261122/1‑138 (MQ=255) ggCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGGAAAGAAAGAGCAAAAACTTCCCTGCAAA‑AAGTTGGGGAAAA‑ATTTGTGGTTTTTTTTTCGCAGGGGAATTCTCtttttg > 1:351472/1‑137 (MQ=255) gtgtCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAAC‑AGGTTCGGAAAAC‑GTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGca < 2:401735/139‑1 (MQ=255) ccTGGCGGGCGATAAATGATTACGGCGGGGTGGGTGCAAAGAAGGAGCAAAAACTGCCCTCAAACAAGGTTGGGAAAA‑‑ATTTTCGTTTTTTTTTTCGCAGGGCAAATTCCTTTTTTTCCGCACTCGCACataataagac > 2:416650/1‑135 (MQ=255) ccTGGCGGGCGATAAATGATTACGGCGGGGTGAGTGGAAAGAAAGAGGAAAAAATACCCTCAAAA‑AAGGTCGGGGAAC‑GATTGCGTTTTTTTTTTCGCCGGGCAATTCCCTTTTTTTTCGAACTCGCCCATAATACAcc > 2:284463/1‑139 (MQ=255) tAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAAC‑AGGTTCGGAAAAC‑GTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGcac < 2:351472/139‑1 (MQ=255) aTTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAAC‑AGGTTCGGAAAAC‑GTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCgg < 1:284463/138‑1 (MQ=255) aTTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAAC‑AGGTTCGGAAAAC‑GTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCgg < 2:332594/138‑1 (MQ=255) gTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAAC‑AGGTTCGGAAAAC‑GTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGgag < 2:56526/139‑1 (MQ=255) tGCAAAGAAGGAGCAAAATCTGCCCTGAAAC‑AGGTTCGGAAAAC‑GTTTGCGTTGTTTTGGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCa < 1:257677/139‑1 (MQ=255) gagggAGCAAAATCTGCCCTGAAAC‑AGGTTCGGAAAAC‑GTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGtact < 2:60262/136‑1 (MQ=255) | AGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAAC‑AGGTTCGGAAAAC‑GTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACT > NZ_CP009273/999969‑1000230 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |