| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,518,384 | 0 | A | G | 58.3% | 3.7 / 12.2 | 13 | T719A (ACC→GCC) | mrcA | peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcA |
| Reads supporting (aligned to +/- strand): ref base A (0/5); new base G (7/0); total (8/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.26e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.93e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATTCCTGATTAAGAGTGCTTTGAACACCAATATCTTTGGTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGTTCTCGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAAC > NZ_CP009273/3518269‑3518490 | aTTCCTGATTAAGAGTGCTTTGAACACCAATATCTTTGGTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAACCGGGACCACTAACAGTTCGAAAGATGCg < 1:137896/139‑1 (MQ=255) aTTAAGAGTGCTTTGAACACCAATATCTTTGGTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGTtct < 2:40525/139‑1 (MQ=255) ttAAGAGTGCTTTGAACACCAATATCTTTGGTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAAACGGGGGCACTAAAAGGTCGGAAGATGCGGGGGtctt > 2:186438/1‑138 (MQ=255) gTGCTTTGAACACCAATATCTTTGGTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAA‑CGGGGCCCCTCAAAGTTCGAAAGATGCGTGGGTCTCGGGGTAc > 2:303510/1‑139 (MQ=255) cTTTGAACACCAATATCTTTGGTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGGAAAAAGGGGACACGCACAAGTTGAAAGAAGCGGGGTTTCCGGGTTACgg > 1:317508/1‑139 (MQ=255) tGAACACCAATATCTTTGGTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGTTCTCGGGTTACGGTc < 1:400952/138‑1 (MQ=255) ggTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAAACCGGGGCACTAACAATTCGCAAGATGCGTGGTTTTCCGGGTACGGGCCGGGGGGTGTGGcccccg > 1:303389/1‑135 (MQ=255) ggCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGGAAAACGGGGCCCCTAACAAGTTGAAAGATGCGGGGGTCTCGGGGTACGGGGCGGGGGGTGGGACCTCGGGCTGGGttt > 1:52935/1‑138 (MQ=255) cAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAAACGGGGGCCCCAACAATTTCAAAAATGCGTGGGTCTCGGGGTTTGGGCCGGGCGGTGTGGGCTCGGGGTGGGTTGGGtttt > 2:393785/1‑138 (MQ=255) tACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGCGGGGAAAACCGGG‑CCACCAAAAGTTCGGAAGAAGCGGGGTTCTCGGGGTACGGTCCGGGCGGTGTGACCTCGGGCTGGGTTGGCTTtggtggt > 1:159666/1‑137 (MQ=255) cAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGTTCTCGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAAtct < 1:135386/139‑1 (MQ=255) cGTGATTTGCAGCGTCGCGATATCGGC‑GGGGAAAACGGGGCCCCCAACAGTTCGGAAGAAGCGTGGGTTTCGGGGTAAGGTCCGGGGGTTGTGACCTCGGGCTGGGTTGGGTTTTGTGGTCACCCTCCGAATTTCgggc > 1:356903/1‑137 (MQ=255) gCAGCGTCGCGATATCGGC‑GGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGTTCTCGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAAc < 1:393785/138‑1 (MQ=255) | ATTCCTGATTAAGAGTGCTTTGAACACCAATATCTTTGGTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGTTCTCGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAAC > NZ_CP009273/3518269‑3518490 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |