| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,597,092 | 0 | A | C | 55.6% | 7.7 / 10.6 | 9 | V171G (GTT→GGT) | ftsY | signal recognition particle‑docking protein FtsY |
| Reads supporting (aligned to +/- strand): ref base A (4/0); new base C (0/5); total (4/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.94e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.17e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCTGATAAATCCGGAACCGAGATTTTCTTTGGTTTTTAACAGGCTGCGTTTCAGGCGCGCGAAAAAACCTTCTTTGGTCGGTTTTTCCTGCTCCTGAGCGATTTCTTCCACCGGCTGCTCTTCTTCTGCCGGAGGAACCACCATCACCGCCTCTTCTGCCGCTTCGGCAGCCAGCGCCGTTTCCAGCTCTTCGTCGGTAATTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTCGACAATCTCTACGGTTTCCGCTTCAGCCTGCCA > NZ_CP009273/3596956‑3597222 | gCTGATAAATCCGGAACCGAGATTTTCTTTGGTTTTTAACAGGCTGCGTTTCAGGCGCGCGAAAAAACCTTCTTTGGTCGGTTTTTCCTGCTCCTGAGCGATTTCTTCCACCGGCTGCTCTTCTTCTGCCGGAGGAac > 1:205216/1‑138 (MQ=255) cgAAAAAACCTTCTTTGGTCGGTTTTTCCTGCTCCTGAGCGATTTCTTCCACCGGCTGCTCTTCTTCTGCCGGAGGAACCACCATCACCGCCTCTTCTGCCGCTTCGGCAGCCAGCGCCGTTTCCAGCTCTTCGTCgg > 1:242603/1‑138 (MQ=255) ggTTTTTCCTGCTCCTGAGCTATTTCTCCCACCGGCTGCTCTTCTTCTGCCGGAGGACCCCCCATCCCCGCCTCTTCTGCCGCTTCGGCAGCCAGCGCCGTTTCCAGCTCTTCGTCGGTAATTTCTTCTTTAGCCGct < 1:193800/138‑1 (MQ=255) gAGCGATTTCTTCCACCGGCTGCTCTTCTTCTGCCGGAGGAACCACCATCACCGCCTCTTCTGCCGCTTCGGCAGCCAGCGCCGTTTCCAGCTCTTCGTCGGTAATTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTc > 1:370113/1‑139 (MQ=255) cgttttCTTCCCCCGCCTGCTCTTCTTCGCCCGGAGCACCCCCCACCACCGCCTCTTCTGCCGCTTCGGCAGCCAGCGCCGTTTCCAGCTCTTCGTCGGTAATTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTCGAc < 2:362186/136‑1 (MQ=255) gcctcctctTTTTCTGCCGTAGGACCCCCCATCACCGCCTCTTCTGCCGCTTCGGCAGCCAGCGCCGTTTCCAGCTCTTCGTCGGTAATTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTCGACAATCTCTACGGttt < 1:423504/134‑1 (MQ=255) gctgctCTTCTTCTGCCGGAGGAACCACCATCACCGCCTCTTCTGCCGCTTCGGCAGCCAGCGCCGTTTCCAGCTCTTCGTCGGTAATTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTCGACAATCTCTACGGTTTc > 1:43205/1‑139 (MQ=255) cgggagaaCCCCCCACCCCCGCCTCTTCTGCCGCTTCGGCAGCCAGCGCCGTTTCCAGCTCTTCGTCGGTAATTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTCGACAATCTCTACGGTTTCCGCTTCAGCCTGCCa < 2:400380/137‑1 (MQ=255) ccGGAGGAACCACCATCACCGCCTCTTCTGCCGCTTCGGCAGCCAGCGCCGTTTCCAGCTCTTCGTCGGTAATTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTCGACAATCTCTACGGTTTCCGCTTCAGCCTGCCa > 2:400755/1‑139 (MQ=255) ccGGAGAACCCCCCACCCCCGCCTCTTCTGCCGCTTCGGCAGCCAGCGCCGTTTCCAGCTCTTCGTCGGTAATTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTCGACAATCTCTACGGTTTCCGCTTCAGCCTGCCa < 2:242603/139‑1 (MQ=255) | GCTGATAAATCCGGAACCGAGATTTTCTTTGGTTTTTAACAGGCTGCGTTTCAGGCGCGCGAAAAAACCTTCTTTGGTCGGTTTTTCCTGCTCCTGAGCGATTTCTTCCACCGGCTGCTCTTCTTCTGCCGGAGGAACCACCATCACCGCCTCTTCTGCCGCTTCGGCAGCCAGCGCCGTTTCCAGCTCTTCGTCGGTAATTTCTTCTTTAGCCGCTTCTTCTTCCGCCGCTTCGACAATCTCTACGGTTTCCGCTTCAGCCTGCCA > NZ_CP009273/3596956‑3597222 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |