| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,116,138 | 0 | T | G | 53.8% | 4.4 / 11.7 | 13 | T201P (ACC→CCC) | priA | primosomal protein N' |
| Reads supporting (aligned to +/- strand): ref base T (4/2); new base G (7/0); total (11/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.66e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGCTAAACTCTGGTGTTTCACTTGCTAAATCACACAGACCTTTTTTGCGTAGCGCCTGCAACGCGGCATCATTAAATTCGA > NZ_CP009273/4116029‑4116266 | aCGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGGTCGGGATTCAAATGCAACCGCTCACCAGGAACgg > 1:205716/1‑139 (MQ=255) tGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGGTCGGGATTCAATCGCAACCGCCCACCAGAAACGGGAt > 1:433932/1‑138 (MQ=255) agaTAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAgt > 1:55702/1‑139 (MQ=255) ccGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGGCTGTTCGGGATTCAAACGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGc > 2:34680/1‑137 (MQ=255) gAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCCGTTCGGGATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGCTAAAct > 2:219088/1‑139 (MQ=255) gccAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTTGGTATTCAATCGCAACCGCTCACCAGAAACGGCCTAGTTCGTTCGCCAGTCGCTAAACTCTGGTGTTTCACTTg > 2:245950/1‑139 (MQ=255) ccAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCCACCGCTCACCCGAAACGGCATAGTTCGTTCGCCCGTCGCTAAAATCTGGTGTTTCACTTGCTAAATca > 2:433716/1‑139 (MQ=255) cgcCAACGGCGGTGGGCTGTTCGGGATTTAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGCTAAAATCTGGTGTTTTACTTGCTAAATCACACAGACCTTTTTTGCGTAGCGCCTGCAACGCgg > 2:366460/1‑138 (MQ=255) aCGGCGGTGGCCTGTTTGGGATTCAATCGCAACCGCTCCCCAGAAAAGGCATAGTTCGTTCGCCCGTCGCTAAACTCTGGTGTTTCACTTGCTAAATCACACAGACCTTTTTTGCGTAGCGCCTGCAACGCGGcatcat > 2:46650/1‑139 (MQ=255) aCGGCGGTGGCCTGTTCGGGATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCCGTCGCTAAACTCTGGTGTTTCACTTGCTAAATCACACAGACCTTTTTTGCGTAGCGCCTGCAACGCGGcatcat > 2:38549/1‑139 (MQ=255) gCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGCTAAACTCTGGTGTTTCACTTGCTAAATCACACAGACCTTTTTTGCGTAGCGCCTGCAACGCGGCATCATTAAATTCGa < 2:55702/139‑1 (MQ=255) ttCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGCTAAACTCTGGTGTTTCACTTGCTAAATCACACAGACCTTTTTTGCGTAGCGCCTGCAACGCGGc < 1:14404/120‑1 (MQ=255) ttCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGCTAAACTCTGGTGTTTCACTTGCTAAATCACACAGACCTTTTTTGCGTAGCGCCTGCAACGCGGc > 2:14404/1‑120 (MQ=255) | ACGCTGAGATAAACCTCCGTTTTACCGGAACCGGTAACGCCCGCCAGCAGCCAGGCAGAAAAAGTATCTGCCGCGCTATGAATTGCGCCAACGGCGGTGGCCTGTTCGGTATTCAATCGCAACCGCTCACCAGAAACGGCATAGTTCGTTCGCCAGTCGCTAAACTCTGGTGTTTCACTTGCTAAATCACACAGACCTTTTTTGCGTAGCGCCTGCAACGCGGCATCATTAAATTCGA > NZ_CP009273/4116029‑4116266 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |