| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,368,619 | 0 | A | C | 55.6% | 3.7 / 10.2 | 9 | V48G (GTA→GGA) | ampC | BlaEC family class C beta‑lactamase |
| Reads supporting (aligned to +/- strand): ref base A (3/1); new base C (0/5); total (3/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.76e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.17e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCC‑TGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCG > NZ_CP009273/4368487‑4368748 | tCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCC‑TGATAAATTaccgcca > 1:120994/1‑139 (MQ=255) tttttGCTCCCCAACCCAAACACAAAAACCGTTTGGTGTGGGGGGGGTTTCTTTTTGGGGATGCCCGCTTACCCCGGGAAAAAGAAAAAAGTTTTCCCC‑TAATAAATCCCCCCCGCCGCCATACCCGGGATCTTttgt < 2:406545/137‑1 (MQ=255) ttttGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGTTTTTTGGCGATGTCCGCATACCCCCAGGTAAATAAATAAGTTTTACCC‑TGATAAATTCCCGCCCCCGCCATACCCGGGATCTTttgtt < 1:366001/138‑1 (MQ=255) ccatcGTTTGCGGTGTGAGGGGCTGTTTTTTGGCGATGTCCCAATAGCCCAAGGTAAAGTAATAGGTTTTACCC‑TAAAAATTTCCCCCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCg < 2:326983/135‑1 (MQ=255) acacggTTTGCTGTGTGACGGGCTGTTTTTTGGGGATGCCCGCATACCCCCAGGAAAAGAAATAGGTTTTCCCC‑TAAAAAATCCCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCg < 2:120994/134‑1 (MQ=255) aaacgttttGGTGTGTGAGGGGTGGTTTTTTGGGGTTGCCCGCATA‑CCCCAAGAAAAATAAAAAAGTTTTCCCATAATAATTCCCCGCCCCCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCg < 2:377183/133‑1 (MQ=255) gtgtgACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCC‑TGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATc > 1:110936/1‑139 (MQ=255) tgACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCC‑TGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGtt > 2:83718/1‑139 (MQ=255) gCCCCAGGTAAAGTAATAAGGTTTACCC‑TGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGc > 2:394509/1‑137 (MQ=255) aaaaaTTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGcg < 2:110936/135‑1 (MQ=255) | TCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCC‑TGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCG > NZ_CP009273/4368487‑4368748 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |