Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,701,793	A→C	P206P (CCA→CCC)	rsxC →	electron transport complex subunit RsxC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,701,793	0	A	C	100.0%	70.7 / NA	21	P206P (CCA→CCC)	rsxC	electron transport complex subunit RsxC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (12/9);  total (12/9)

GCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCACGCGAAATTCTTATCGGCATTGAAGATAACAAACCGCAGGCGATTTCCATGCTGCGCGCGGTGCTGGCGGACTCTAACGATATTTCTCTGCGGGTGATTCCAACCAAATATCCTTCTGGCGGTGCTAAACAA  >  NZ_CP009273/1701657‑1701925                                                                                                                                         |                                                                                                                                     gCGGAAAAAAGTTTGAAACGTTGATTACAAACGCGGCGGAGTGCGACCCTTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCCcg                                                                                                                                    <  2:183038/139‑1 (MQ=255)  cGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCCcgc                                                                                                                                   >  1:254865/1‑139 (MQ=255)       aTAAGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCCCGCGAAAt                                                                                                                              >  1:260141/1‑139 (MQ=255)             ttGAAACGTTGATTATCAACGCGGCTGGGTGGGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCCCGCGAAATTCTTAt                                                                                                                        <  1:24221/139‑1 (MQ=255)                                        ggggCGAGCCGTACTTTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCCCGCGAAATTCTTATCGGCATTGAAGATAACAAACCGCAGGc                                                                                             <  2:260141/136‑1 (MQ=255)                                              aGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCCCGCGAAATTCTTATCGGCATTGAAGATAACAAACCGCAGGCGATTTc                                                                                       >  1:137551/1‑139 (MQ=255)                                                    aCATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCCCGCGAAATTCTTATCGGCATTGAAGATAACAAACCGCAGGCGATTTCCAtgct                                                                                 >  2:241810/1‑139 (MQ=255)                                                             ccgATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCCCGCGAAATTCTTATCGGCATTGAAGATAACAAACCGCAGGCGATTTCCATGCTGCGCGCGGt                                                                        >  2:289549/1‑139 (MQ=255)                                                                       tttGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCCCGCGAAATTCTTATCGGCATTGAAGATAACAAACCGCAGGCGATTTCCATGCTGCGCGCGGTGCTGGCg                                                                 >  2:41286/1‑136 (MQ=255)                                                                       tttGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCCCGCGAAATTCTTATCGGCATTGAAGATAACAAACCGCAGGCGATTTCCATGCTGCGCGCGGTGCTGGCg                                                                 <  1:41286/136‑1 (MQ=255)                                                                                  aTTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCCCGCGAAATTCTTATCGGCATTGAAGATAACAAACCGCAGGCGATTTCCATGCTGCGCGCGGTGCTGGCGGACTCTAACGatat                                                   <  1:76444/139‑1 (MQ=255)                                                                                      cgcgGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCCCGCGAAATTCTTATCGGCATTGAAGATAACAAACCGCAGGCGATTTCCATGCTGCGCGCGGTGCTGGCGGACTCTAACGATATTtct                                               >  1:328020/1‑139 (MQ=255)                                                                                                          gTATTCGCATTCTTGCGCATATTCTGCAGCCCCGCGAAATTCTTATCGGCATTGAAGATAACAAACCGCAGGCGATTTCCATGCTgcgcg                                                                            >  1:421748/1‑90 (MQ=255)                                                                                                          gTATTCGCATTCTTGCGCATATTCTGCAGCCCCGCGAAATTCTTATCGGCATTGAAGATAACAAACCGCAGGCGATTTCCATGCTgcgcg                                                                            <  2:421748/90‑1 (MQ=255)                                                                                                          gTATTCGCATTCTTGCGCATATTCTGCAGCCCCGCGAAATTCTTATCGGCATTGAAGATAACAAACCGCAGGCGATTTCCATGCTGCGCGCGGTGCTGGCGGACTCTAACGATATTTCTCTGCGGGTGATTcca                                <  1:394663/134‑1 (MQ=255)                                                                                                          gTATTCGCATTCTTGCGCATATTCTGCAGCCCCGCGAAATTCTTATCGGCATTGAAGATAACAAACCGCAGGCGATTTCCATGCTGCGCGCGGTGCTGGCGGACTCTAACGATATTTCTCTGCGGGTGATTcca                                >  2:394663/1‑134 (MQ=255)                                                                                                              tCGCATTCTTGCGCATATTCTGCAGCCCCGCGAAATTCTTATCGGCATTGAAGATAACAAACCGCAGGCGATTTCCATGCTGCGCGCGGTGCTGGCGGACTCTAACGATATTTCTCTGCGGGTGATTCCAACCAAatat                       >  1:101425/1‑139 (MQ=255)                                                                                                                   ttCTTGCGCATATTCTGCAGCCCCGCGAAATTCTTATCGGCATTGAAGATAACAAACCGCAGGCGATTTCCATGCTGCGCGCGGTGCTGGCGGACTCTAACGATATTTCTCTGCGGGTGATTCCAACCAAATATCCTTc                  >  1:369065/1‑139 (MQ=255)                                                                                                                        gcgcATATTCTGCAGCCCCGCGAAATTCTTATCGGCATTGAAGATAACAAACCGCAGGCGATTTCCATGCTGCGCGCGGTGCTGGCGGACTCTAACGATATTTCTCTGCGGGTGATTCCAACCAAATATCCTTCTgg               <  2:137551/137‑1 (MQ=255)                                                                                                                        gcgcATATTCTGCAGCCCCGCGAAATTCTTATCGGCATTGAAGATAACAAACCGCAGGCGATTTCCATGCTGCGCGCGGTGCTGGCGGACTCTAACGATATTTCTCTGCGGGTGATTCCAACCAAATATCCTTCTgg               <  2:369065/137‑1 (MQ=255)                                                                                                                                   gCAGCCCCGCGAAATTCTTATCGGCATTGAAGATAACAAACCGCAGGCGATTTCCATGCTGCGCGCGGTGCTGGCGGACTCTAACGATATTTCTCTGCGGGTGATTCCAACCAAATATCCTTCTGGCGGTGCTAAACaa  >  2:189939/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                     GCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCACGCGAAATTCTTATCGGCATTGAAGATAACAAACCGCAGGCGATTTCCATGCTGCGCGCGGTGCTGGCGGACTCTAACGATATTTCTCTGCGGGTGATTCCAACCAAATATCCTTCTGGCGGTGCTAAACAA  >  NZ_CP009273/1701657‑1701925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑