Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,708,538	A→G	intergenic (+10/‑96)	dtpA → / → gstA	dipeptide/tripeptide permease DtpA/glutathione transferase GstA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,708,538	0	A	G	100.0%	29.0 / NA	10	intergenic (+10/‑96)	dtpA/gstA	dipeptide/tripeptide permease DtpA/glutathione transferase GstA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/4);  total (6/4)

CGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTACTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCG  >  NZ_CP009273/1708402‑1708670                                                                                                                                         |                                                                                                                                     cgTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTACTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGgaa                                                                                                                                    <  1:308307/139‑1 (MQ=255)                            tGCCGTTATTGCAGTACTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCGCTAACTTTTCGt                                                                                                         >  2:395545/1‑139 (MQ=255)                                               aTGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGtt                                                                                      >  2:284531/1‑139 (MQ=255)                                                  ctgctgACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAg                                                                                   <  2:241185/139‑1 (MQ=255)                                                         ccGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCa                                                                            <  1:115959/139‑1 (MQ=255)                                                                            cATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCg                                                                                                                     <  1:274662/79‑1 (MQ=255)                                                                            cATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCg                                                                                                                     >  2:274662/1‑79 (MQ=255)                                                                            cATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACca                                                          >  2:419609/1‑138 (MQ=255)                                                                                    aGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGgaa                                                                                                                                    <  1:345508/56‑1 (MQ=255)                                                                                    aGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGgaa                                                                                                                                    >  2:345508/1‑56 (MQ=255)                                                                                       aTGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAg                                                >  2:46364/1‑137 (MQ=255)                                                                                                cAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGGAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGAt                                     >  2:388629/1‑139 (MQ=255)                                                                                                                                    cAGGGGAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCg  <  1:16566/138‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     CGTATTCTTGCAGATTGGTGTCGCTACTGCCGTTATTGCAGTACTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCG  >  NZ_CP009273/1708402‑1708670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑