Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,737,390	T→C	E37G (GAA→GGA)	ribE ←	riboflavin synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,737,390	0	T	C	100.0%	52.9 / NA	16	E37G (GAA→GGA)	ribE	riboflavin synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/8);  total (8/8)

CCCAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAA  >  NZ_CP009273/1737256‑1737486                                                                                                                                       |                                                                                                 cccAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCAg                                                                                              >  1:305032/1‑139 (MQ=255)     aTCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCg                                                                                          >  2:163965/1‑139 (MQ=255)         cccACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTCCa                                                                                      <  2:44967/139‑1 (MQ=255)                        ccAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTAACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTc                                                                                        >  1:291701/1‑122 (MQ=255)                        ccAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTAACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTc                                                                                        <  2:291701/122‑1 (MQ=255)                                       cGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTCCAGCAt                                                                                  >  1:345248/1‑113 (MQ=255)                                       cGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTCCAGCAt                                                                                  <  2:345248/113‑1 (MQ=255)                                       cGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCg                                                        >  2:270110/1‑139 (MQ=255)                                       cGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCg                                                        >  2:27925/1‑139 (MQ=255)                                                                     aCATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGaca                          >  2:197785/1‑139 (MQ=255)                                                                                gTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCg               <  1:163965/139‑1 (MQ=255)                                                                                gTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCg               <  1:235773/139‑1 (MQ=255)                                                                                gTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCg               <  1:270110/139‑1 (MQ=255)                                                                                gTTAATTTCCGCCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCg               <  1:401966/139‑1 (MQ=255)                                                                                                           aCCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACaa  <  1:48226/125‑1 (MQ=255)                                                                                                           aCCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACaa  >  2:48226/1‑125 (MQ=255)                                                                                                                                       |                                                                                                 CCCAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTGCCCTGTACAA  >  NZ_CP009273/1737256‑1737486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑