Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,765,154	A→G	N95D (AAT→GAT)	ydiL →	YdiL family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,765,154	0	A	G	100.0%	72.0 / NA	21	N95D (AAT→GAT)	ydiL	YdiL family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/10);  total (11/10)

GTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCGGCTTTGTTAATTTTTCCACAGAAAGGAATTGTCGTTGTTACAACAATAATGAACGGATGCTGACACAA  >  NZ_CP009273/1765024‑1765286                                                                                                                                   |                                                                                                                                     gTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTAtt                                                                                                                              >  1:129761/1‑139 (MQ=255)  ttGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAAAAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTAtt                                                                                                                              <  1:94906/138‑1 (MQ=255)         tGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGa                                                                                                                       <  2:371170/138‑1 (MQ=255)            gTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTgg                                                                                                                        >  2:375545/1‑134 (MQ=255)               aGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCt                                                                                                                 <  1:375545/138‑1 (MQ=255)                 cAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTAt                                                                                                               >  1:268221/1‑138 (MQ=255)                        aaaaaCATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGt                                                                                                       <  2:268221/139‑1 (MQ=255)                                          tcatAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGc                                                                                     <  2:149666/139‑1 (MQ=255)                                           catAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGca                                                                                    >  1:316886/1‑139 (MQ=255)                                                            aCCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCGGCt                                                                   >  1:274500/1‑139 (MQ=255)                                                                cATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCGGCTTTGt                                                               >  2:207630/1‑139 (MQ=255)                                                                         caacaCCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTTCCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCGGCTTTGTTAATTTTTc                                                      <  2:397463/139‑1 (MQ=255)                                                                         caacaCCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCGGCTTTGTTAATTTTTc                                                      <  2:129761/139‑1 (MQ=255)                                                                           acacCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCGGCTTTGTTAATTTTTCca                                                    <  2:316886/139‑1 (MQ=255)                                                                                 tGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGt                                                                                                       <  1:339460/82‑1 (MQ=255)                                                                                 tGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGt                                                                                                       >  2:339460/1‑82 (MQ=255)                                                                                 tGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCGGCTTTGTTAATTTTTCCACAGa                                                >  2:399576/1‑137 (MQ=255)                                                                                 tGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCGGCTTTGTTAATTTTTCCACAGa                                                >  1:277977/1‑137 (MQ=255)                                                                                               gACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCGGCTTTGTTAATTTTTCCACAGAAAGGAATTGTCgttgt                                <  2:217773/139‑1 (MQ=255)                                                                                                           tttCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCGGCTTTGTTAATTTTTCCACAGAAAGGAATTGTCGTTGTTACAACaataat                    >  2:120786/1‑139 (MQ=255)                                                                                                                              aTGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCGGCTTTGTTAATTTTTCCACAGAAAGGAATTGTCGTTGTTACAACAATAATGAACGGATGCTGACacaa  >  1:132081/1‑138 (MQ=255)                                                                                                                                   |                                                                                                                                     GTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCGGCTTTGTTAATTTTTCCACAGAAAGGAATTGTCGTTGTTACAACAATAATGAACGGATGCTGACACAA  >  NZ_CP009273/1765024‑1765286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑