Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,769,436	A→G	D165G (GAC→GGC)	aroD →	type I 3‑dehydroquinate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,769,436	0	A	G	100.0%	56.3 / NA	17	D165G (GAC→GGC)	aroD	type I 3‑dehydroquinate dehydratase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/9);  total (8/9)

TGATCAGGTTAAAGAAACCGTCGCCTACGCCCACGCGCATGATGTGAAAGTAGTCATGTCCAACCATGACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGACGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCCGCGACCCTGGAGATGCAGGAGCAGTATGCCGATCGTCCAATTATCACGATGT  >  NZ_CP009273/1769305‑1769552                                                                                                                                    |                                                                                                                     tgatCAGGTTAAAGAAACCGTCGCCTACGCCCACGCGCATGATGTGAAAGTAGTCATGTCCAACCATGACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGat                                                                                                               <  2:373165/139‑1 (MQ=255)                 aCCGTCGCCTACGCCCACGCGCATGATGTGAAAGTAGTCATGTCCAACCATGACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTgcgc                                                                                               >  1:275859/1‑139 (MQ=255)                  ccGTCGCCTACGCCCACGCGCATGATGTGAAAGTAGTCATGTCCAACCATGACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCt                                                                                              >  1:205047/1‑139 (MQ=255)                    gTCGCCTACGCCCACGCGCATGATGTGAAAGTAGTCATGTCCAACCATGACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGa                                                                                            <  2:205047/139‑1 (MQ=255)                                      cATGATGTGAAAGTAGTCATGTCCAACCATGACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGc                                                                           >  1:185636/1‑138 (MQ=255)                                                                ccATGTCTTCCATAAATCGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGTTGACGTTGCTTGCCGCGAcc                                                <  1:208353/139‑1 (MQ=255)                                                                 cATGACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCCGCGAccc                                               <  2:150509/139‑1 (MQ=255)                                                                     aCTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCCGCGACCCTGga                                           <  1:140610/139‑1 (MQ=255)                                                                            tAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCCGCGACCCTGGAGATGCAg                                    <  2:7603/139‑1 (MQ=255)                                                                               aaCGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCCGCGACCCTGGAGATGCAGGAg                                 <  2:275859/139‑1 (MQ=255)                                                                                aCGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCCGCGACCCTGGAGATGCAGGAGc                                <  2:177009/139‑1 (MQ=255)                                                                                    cGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCCGCGACCCTGGAGATGCAGGAGCAGTa                            >  2:328445/1‑139 (MQ=255)                                                                                    cGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCCGCGACCCTGGAGATGCAGGAGCAGTa                            >  2:346685/1‑139 (MQ=255)                                                                                    cGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCCGCGACCCTGGAGATGCAGGAGCAGTa                            >  2:379775/1‑139 (MQ=255)                                                                                           cGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCCGCGACCCTGGAGATGCAGGAGCAGTATGCCGAt                     <  1:328445/139‑1 (MQ=255)                                                                                                  aTCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCCGCGACCCTGGAGATGCAGGAGCAGTATGCCGATCGTCCaa              >  2:180165/1‑139 (MQ=255)                                                                                                             tCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCCGCGACCCTGGAG‑TGCAGGAGCAGTATGCCGATCGTCCAATTATCACGATGt  >  2:11079/1‑139 (MQ=255)                                                                                                                                    |                                                                                                                     TGATCAGGTTAAAGAAACCGTCGCCTACGCCCACGCGCATGATGTGAAAGTAGTCATGTCCAACCATGACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGACGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCCGCGACCCTGGAGATGCAGGAGCAGTATGCCGATCGTCCAATTATCACGATGT  >  NZ_CP009273/1769305‑1769552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑