Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,776,037	T→G	V129G (GTT→GGT)	ydiS →	FAD‑dependent oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,776,037	0	T	G	100.0%	51.9 / NA	16	V129G (GTT→GGT)	ydiS	FAD‑dependent oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (11/5);  total (11/5)

TCCGCAACACGCATCTTATACCGTATTGCGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGTTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCC  >  NZ_CP009273/1775942‑1776172                                                                                                |                                                                                                                                        tCCGCAACACGCATCTTATACCGTATTGCGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACCAGGTCACTGGc                                                                                              >  2:259488/1‑139 (MQ=255)                     ccGTATTGCGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGata                                                                           <  2:245008/138‑1 (MQ=255)                        tATTGCGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCCGGCGGGCGCGCAGTTTATCCCCGGCGGACCCGTCGACGCGCTTGTTTGTGCAGGAAAAAAGGTCCACGGCGTGGCCGCTGGGGTAGATATtct                                                                       >  2:258779/1‑139 (MQ=255)                        tATTGCGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGGAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATtct                                                                       >  1:189100/1‑139 (MQ=255)                        tATTGCGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGGGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATtct                                                                       >  1:334843/1‑139 (MQ=255)                         aTTGCGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGaa                                                                                                                >  1:317685/1‑97 (MQ=255)                         aTTGCGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGaa                                                                                                                <  2:317685/97‑1 (MQ=255)                             cGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAg                                                                  >  1:103029/1‑139 (MQ=255)                             cGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAg                                                                  >  1:364914/1‑139 (MQ=255)                                aaTCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGGTGATATTCTCGAAGCg                                                                >  2:188653/1‑138 (MQ=255)                                  tCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAAt                                                             >  1:162772/1‑139 (MQ=255)                                                              gCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGGAGGAAACAAGGTCACTGGCGGGCAGGCTGGGGATGATATTCTCGGAGCGAATGTGGTGATTCTGGCTGATGGCGGTAACt                                 >  1:32727/1‑139 (MQ=255)                                                              gCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGaa                                                                   >  1:49338/1‑105 (MQ=255)                                                              gCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGaa                                                                   <  2:49338/105‑1 (MQ=255)                                                              gCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACt                                 <  2:162772/139‑1 (MQ=255)                                                                                             gCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTcc  <  2:334843/139‑1 (MQ=255)                                                                                                |                                                                                                                                        TCCGCAACACGCATCTTATACCGTATTGCGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGTTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCC  >  NZ_CP009273/1775942‑1776172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑