Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,809,395	T→G	G424G (GGT→GGG)	katE →	catalase HPII

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,809,395	0	T	G	100.0%	20.8 / NA	7	G424G (GGT→GGG)	katE	catalase HPII
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (5/2);  total (5/2)

CCGGGACTGGACTTCACCAACGATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAA  >  NZ_CP009273/1809315‑1809511                                                                                 |                                                                                                                     ccGGGACTGGACTTCACCAACGATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTc                                                             <  1:156880/138‑1 (MQ=255)          gactTCACCAACGATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACg                                                   >  2:126555/1‑139 (MQ=255)                                                      taCCGATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTAcgaa       >  1:394153/1‑139 (MQ=255)                                                           atgcacaAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGcc                                                                           <  1:176595/63‑1 (MQ=255)                                                           aTACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGcc                                                                           >  2:176595/1‑66 (MQ=255)                                                           aTACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTAcgaaccgaa  >  2:424317/1‑139 (MQ=255)                                                           aTACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTAcgaaccgaa  >  2:90693/1‑139 (MQ=255)                                                                                 |                                                                                                                     CCGGGACTGGACTTCACCAACGATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAA  >  NZ_CP009273/1809315‑1809511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑